Wounding stimulates the accumulation of glycerolipids containing oxophytodienoic acid and dinor-oxophytodienoic acid in Arabidopsis leaves. Plant Physiol

Division of Biology, Kansas State University, Manhattan, 66506, USA.
Plant physiology (Impact Factor: 7.39). 10/2006; 142(1):28-39. DOI: 10.1104/pp.106.082115
Source: PubMed

ABSTRACT Although oxylipins can be synthesized from free fatty acids, recent evidence suggests that oxylipins are components of plastid-localized polar complex lipids in Arabidopsis (Arabidopsis thaliana). Using a combination of electrospray ionization (ESI) collisionally induced dissociation time-of-flight mass spectrometry (MS) to identify acyl chains, ESI triple-quadrupole (Q) MS in the precursor mode to identify the nominal masses of complex polar lipids containing each acyl chain, and ESI Q-time-of-flight MS to confirm the identifications of the complex polar lipid species, 17 species of oxylipin-containing phosphatidylglycerols, monogalactosyldiacylglycerols (MGDG), and digalactosyldiacylglycerols (DGDG) were identified. The oxylipins of these polar complex lipid species include oxophytodienoic acid (OPDA), dinor-OPDA (dnOPDA), 18-carbon ketol acids, and 16-carbon ketol acids. Using ESI triple-Q MS in the precursor mode, the accumulation of five OPDA- and/or dnOPDA-containing MGDG and two OPDA-containing DGDG species were monitored as a function of time in mechanically wounded leaves. In unwounded leaves, the levels of these oxylipin-containing complex lipid species were low, between 0.001 and 0.023 nmol/mg dry weight. However, within the first 15 min after wounding, the levels of OPDA-dnOPDA MGDG, OPDA-OPDA MGDG, and OPDA-OPDA DGDG, each containing two oxylipin chains, increased 200- to 1,000-fold. In contrast, levels of OPDA-hexadecatrienoic acid MGDG, linolenic acid (18:3)-dnOPDA MGDG, OPDA-18:3 MGDG, and OPDA-18:3 DGDG, each containing a single oxylipin chain, rose 2- to 9-fold. The rapid accumulation of high levels of galactolipid species containing OPDA-OPDA and OPDA-dnOPDA in wounded leaves is consistent with these lipids being the primary products of plastidic oxylipin biosynthesis.

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Available from: Ruth Welti, Aug 29, 2015
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    • "escribed as free oxylipins ( Bohlmann et al . , 1982 , 1983 ; Ohashi et al . , 2005 ; Tamayo - Castillo et al . , 1989 ; Zdero et al . , 1991 ) , as oxylipins esterified with a number of other mole - cules in the cell , like glycerolipids ( glycolipids , phospholipids , and neutral lipids ) ( Andersson et al . , 2006 ; Bottcher and Weiler , 2007 ; Buseman et al . , 2006 ; Göbel and Feussner , 2009 ; Stelmach et al . , 2001 ) , and oxylipins conjugated to amino acids and other metabolites , such as sulfate , glutathione , ethanolamine , and carbo - hydrates ( Mosblech et al . , 2009 ) . The compounds present in the exudate material of S . adenophora were free oxylipins . Compound 5 was isolated as an am"
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    Phytochemistry 11/2014; 110. DOI:10.1016/j.phytochem.2014.10.033 · 3.35 Impact Factor
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    • "Scanning for NL 439.3 (18:3-containing Gal) in samples from wheat 45 min after wounding produced a massive peak at m/z 1052.8, whose signal accounted for 72% of the total acMGDG signal (Table S2). Accurate-mass product ion analysis of this species (acetate adduct, [M + C 2 H 3 O 2 ] − , m/z 1093.8) by Q-TOF MS in the negative mode (Buseman et al. 2006, Vu et al. 2012) showed that this largest acMGDG component of wheat contained only 18:3 acyl chains, consistent with a structure of 1,2- di18:3,3-(18:3-galactosyl) glycerol (Fig. S1). Table S3 shows the acyl composition (three chains) of the major acMGDG molecular species detected in Arabidopsis Col-0, tomato and wheat and the supporting accuratemass product ion analysis. "
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