Lipid lowering effects of Momordica charantia (Bitter Melon) in HIV-1-protease inhibitor-treated human hepatoma cells, HepG2

Laboratory of Metabolic Disorders and Alternative Medicine, Department of Molecular Biosciences and Bioengineering, College of Tropical Agriculture and Human Resources, Room 415H, East-West Road, Honolulu, HI 96822, USA.
British Journal of Pharmacology (Impact Factor: 4.99). 09/2006; 148(8):1156-64. DOI: 10.1038/sj.bjp.0706821
Source: PubMed

ABSTRACT 1. Hyperlipidemic effects of HIV-1-protease inhibitors (PI) are associated with increased hepatic production of triglyceride (TG)-rich lipoproteins, rather than lipoprotein clearance. PI are known to increase apolipoprotein B (apoB) secretion, apoC-III mRNA expression and decrease apoA-1 secretion. Nutritional therapy remains an important strategy to manage PI-associated hyperlipidemia. 2. This study investigated the in vitro efficacy of Asian vegetable, Momordica charantia or bitter melon (BM) to ameliorate PI-associated apoB and lipid abnormalities in HepG2 cells. 3. Our study demonstrates that bitter melon juice (BMJ) significantly reduced apoB secretion and apoC-III mRNA expression and normalized apoA-I expression in PI-treated HepG2 cells. BMJ also significantly reduced cellular TG and microsomal TG transfer protein, suggesting that lipid bioavailability and lipidation of apoB assembly may play a role in decreased apoB secretion. 4. Identifying molecular targets of BM may offer alternative dietary strategies to decrease PI-associated hyperlipidemia and improve quality of life among HIV-1-infected patients.

1 Follower
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Momordica charantia Linn. which has been used mainly for edible purposes at different countries of the world including South East Asia and has also been extensively used in traditional medicines for the cure of various ailments. M. charantia belongs to the cucurbitaceae family. Extensive research has been carried out on the fruit, leaves, and seeds of the plant. Most importantly, all these research works have shown its efficacy on various cancer cell lines like lymphoid leukemia, lymphoma, choriocarcinoma, melanoma, breast cancer, skin tumor, prostatic cancer, squamous carcinoma of tongue and larynx, human bladder carcinomas and on Hodgkin's disease. Clinical reports of some research on the use of M. charantia in diabetes and cancer patients showed promising results. The main active constituents of M. charantia are cucurbitane type triterpenoids which have some potent biological and pharmacological activities including antidiabetic, anti-obesity, anticancer, anti-HIV, anti-feedant and anti-oviposition activities. Since in the early 1960's the constituents of M. charantia have been investigated and several classes of secondary metabolites including cucurbitane-type triterpenoids, glycosides and phenolic compounds have been isolated and their structures were determined. This review summarizes the previous and current information regarding phytochemical constituents of M. charantia and their pharmacological effects that provide the scope for future research in this aspect.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This study aimed to investigate the changes in the proteome of bitter gourd prior to and after subjecting to boiling and microwaving. A comparative analysis of the proteome profiles of raw and thermally treated bitter gourds was performed using 2D-DIGE. The protein content and number of protein spots in raw sample was higher when compared to the cooked samples. Qualitative analysis revealed that 103 (boiled sample) and 110 (microwaved sample) protein spots were up regulated whereas 120 (boiled sample) and 107 (microwaved sample) protein spots were down regulated. Ten protein spots with the highest significant fold change in the cooked samples were involved in carbohydrate/energy metabolisms and stress responses. Small heat shock proteins, superoxide dismutase, quinone oxidoreductase, UDP-glucose pyrophosphorylase and phosphoglycerate kinase play a role in heat-stress-mediated protection of bitter gourd. This study suggests that appropriate heat treatment (cooking methods) can lead to induction of selected proteins in bitter gourd.
    Food Chemistry 04/2014; 148C:155-161. DOI:10.1016/j.foodchem.2013.10.025 · 3.26 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The use of food and food products as medicine has been in practice over centuries in many civilizations. The extracts from the family Cucurbitaceae have been used in the preparation of medicines for a variety of diseases in Ayurveda and ancient Chinese medicine. The article focuses on medicinal properties of extracts derived from different vegetative parts of three Cucurbitaceae species viz. ash gourd (Benincasa hispida), bottle gourd (Lagenaria siceraria) and bitter gourd (Momordica charantia). The competency of extracts derived from these plants using different extraction solutions and techniques were tested against various diseases. These plants were found to possess antioxidant activity, analgesic and anti-inflammatory activity, central nervous system activity, antihyperglycemic and antidiabetics, anti-hyperlipidemic activity, antimicrobial activity and anthelmintic activity, cytotoxic and anticancer activity, immunomodulatory effect, cardioprotective effects, hepatoprotective activity, bronchospasm protective activity, antidiarrheal activity, and diuretic activity.
    01/2013; DOI:10.1016/j.bionut.2013.03.004


1 Download
Available from