A selective fluorescent sensor for detecting lead in living cells.

Qiwen He, Evan W Miller, Audrey P Wong, Christopher J Chang

Department of Chemistry, University of California, Berkeley, California 94720, USA.

Journal Article: Journal of the American Chemical Society (impact factor: 8.58). 08/2006; 128(29):9316-7. DOI: 10.1021/ja063029x

Abstract

We present the synthesis, properties, and biological applications of Leadfluor-1 (LF1), a new water-soluble, turn-on fluorescent sensor that is capable of selectively imaging Pb2+ in aqueous solution and in living cells. LF1 combines a fluorescein chromophore and a pseudocrown receptor to provide good selectivity for Pb2+ over a range of biologically and environmentally relevant metal ions in aqueous solution, with sensitivity to parts per billion EPA limits for allowable lead in drinking water. In addition to these attributes, imaging experiments further show that LF1 is the first small-molecule reagent that can be delivered into living cells and report changes in intracellular Pb2+ levels.

Source: PubMed

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Keywords

allowable lead
 
aqueous solution
 
billion EPA limits
 
biological applications
 
biologically
 
drinking water
 
environmentally relevant metal ions
 
first small-molecule reagent
 
imaging experiments
 
pseudocrown receptor
 
report changes
 
selectively imaging Pb2+