Article

Dog echinococcosis in northern Spain: comparison of coproantigen and serum antibody assays with coprological exam.

Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country, P.O. Box 450, 01080 Vitoria, Spain.
Veterinary Parasitology (Impact Factor: 2.38). 12/2006; 142(1-2):102-11. DOI: 10.1016/j.vetpar.2006.06.011
Source: PubMed

ABSTRACT A large sheep-dog population from the province of Alava (northern Spain) has been investigated in order to determine the prevalence of the cestode parasite Echinococcus granulosus. Worms were detected in 14.0% of 721 dog faecal supernatants by coproantigen ELISA, and in 9.1% of 754 dog serum samples by serum antibody ELISA. A weak but statistically significant correlation (Spearman's rho=0.103, 95% CI: 0.023-0.178) between the two immunoassay results was found. In addition, eggs of the family Taeniidae were detected in 10.3% of 726 faecal samples examined by coproparasitological (flotation and sedimentation) tests. The overall E. granulosus infection rate, based on a Bayesian latent class model that accounts for the imperfect sensitivities and specificities of all diagnostic tests used, was estimated to be 8.0% (95% credible interval: 5.4-11.4%), corroborating that sheep-dog is the dog class most vulnerable to acquiring the infection. Dog sex did not influence the prevalence of E. granulosus, independently of the diagnostic test used or the dog region of origin. No significant linear correlation was found between the coproantigen ELISA OD values and the dog age (Spearman's rho=-0.049, 95% CI: -0.234 to 0.135), suggesting that there were no differences in prevalence of E. granulosus between old and young dogs. The obtained results highlight the importance of initiating a control program based on regular treatment of the sheep-dogs with praziquantel in the province of Alava.

0 Bookmarks
 · 
86 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: Coproantigen test kits for Echinococcus spp. worms in dogs, designed for commercial use, were obtained from three different Chinese producers, and were compared with a laboratory kit using reagents from New Zealand. None of the three producers would provide details of their test validation. From a known set of dog faeces obtained at necropsy from infected and uninfected dogs, and from faeces collected from dogs necropsied in the field, results differed between the kits. For field material, the Tiankang kit showed the best specificity but lacked sensitivity. The Combined kit showed best sensitivity but lacked specificity. Results for the Haitai kit were intermediate. With samples from experimentally infected dogs, both the Haitai and Combined kits lacked sensitivity. Kits will need to be validated by the user before they can be relied on to predict progress in Echinococcus spp. control in China or in other countries.
    Journal of Helminthology 01/2013; · 1.16 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: La �Sección de Caninos, Felinos y Batracios� del Bioterio la Facultad de Ciencias Veterinarias de la Universidad Central de Venezuela, cumple un papel fundamental en la docencia y la investigación en esta institución. Las gastroenteritis parasitarias, son uno de los problemas sanitarios en los caninos, cuyo control requiere de la aplicación de medidas integrales, con conocimiento epidemiológico de estas infecciones. Con el objetivo de obtener información acerca de la prevalencia y géneros parasitarios presentes, se llevó a cabo un estudio de la población de este bioterio conformada por 64 caninos mestizos, mayores de un año de edad y de ambos sexos. Mensualmente durante cinco meses, se recolectaron a primera hora de la mañana las heces que estaban presentes en cada jaula, para un total de 141 muestras, que se procesaron mediante las técnicas de Mc Master Modificada y Baerman. Se obtuvo un valor de prevalencia promedio general de infecciones con parásitos gastrointestinales durante todo el estudio de 58,10%, con prevalencias promedio mensuales entre 38,88 y 65,62%. Los valores promedio de prevalencia y rangos correspondientes de acuerdo al tipo de parásitos fueron: nematodes: Ancylostoma spp= 56,59% (33,33 a 65,62%),Strongyloides stercolaris=11,80% (0-25%) y Toxocara spp=3,80% (0-11%); se observaron además ooquistes de protozoarios del género Cystoisospora (Isospora)=19,82% (2-17%). Sólo hubo diferencias en la prevalencia de S. stercolaris por mes (p<0,05), debido probablemente a la mayor humedad ambiental durante los meses noviembre y diciembre por la presencia de lluvias; no se encontraron diferencias en la prevalencia parasitaria por sexo de hospedador (p>0,05).
    Revista de la Facultad de Ciencias Veterinarias, ISSN 0258-6576, Vol. 49, Nº. 2, 2009, pags. 91-98. 01/2008;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Canine echinococcosis is a potential zoonotic infection caused by the adult form of several cestode species belonging to the genus Echinococcus, of which E. granulosus sensu lato and E. multilocularis are the most epidemiologically relevant. Dogs infected with E. granulosus and E. multilocularis are widely regarded as the main source of infection for human cystic and alveolar echinococcosis, diseases that cause substantial morbidity and socio-economic burden in several regions of the world. Following our previous review on the global situation of cystic echinococcosis in livestock species (Cardona and Carmena Vet. Parasitol. 2013; 192, 10-32), we summarize here current knowledge on the global epidemiology, geographical distribution and molecular diversity of Echinococcus spp. infection in dogs. We address relevant topics including the implications of the increasing urbanization of wildlife species such as foxes, coyotes, and dingoes in the establishment of urban cycles of Echinococcus spp, or the rising concerns regarding the role of unsupervised translocation of infected dogs in spreading the infection to Echinococcus-free areas. The involvement of wildlife species as natural reservoirs of disease to domestic animals and humans and the epidemiological significance of the sympatric occurrence of different Echinococcus species in the same geographical region are also debated. Data presented are expected to be useful for policy makers, educational and health authorities responsible for designing and implementing effective measures for disease control and prevention.
    Acta tropica 08/2013; · 2.79 Impact Factor

Full-text (2 Sources)

View
42 Downloads
Available from
Jun 5, 2014