GSTM1 and GSTT1 null genotypes as possible heritable factors of rosacea

Baskent University, Engüri, Ankara, Turkey
Photodermatology Photoimmunology and Photomedicine (Impact Factor: 1.26). 08/2006; 22(4):208-10. DOI: 10.1111/j.1600-0781.2006.00220.x
Source: PubMed


Rosacea might be related to an increased activity of reactive oxygen species (ROS) and deficient function of the antioxidant system. Glutathione S-transferases (GSTs) play a primer role in cellular defense against electrophilic chemical species and radical oxygen species. We hypothesized that increased ROS activity or decreased antioxidant potential, possibly induced by GST gene polymorphism, might have a pathogenic role in rosacea.
The study group consisted of 45 patients with rosacea and 100 control subjects. DNA samples were isolated from blood samples using high pure polymerase chain reaction (PCR) Template preparation Kit. The GSTM1, GSTT1, and P1 polymorphisms were detected using a real-time PCR and fluorescence resonance energy transfer with a Light-Cycler Instrument. Associations between specific genotypes and the development of rosacea were examined using logistic regression analyses to calculate odds ratios (OR) and 95% confidence intervals (CI).
GSTM1 and GSTT1 null genotypes were found to be statistically different from control (P=0.005, P=0.009, respectively), and associated with an increased risk of rosacea (OR [95% CI]: 2.84 [1.37-5.89]; OR [95% CI]: 2.68 [1.27-5.67], respectively). There was a statistically significant relationship between both null combination of the GSTM1 and GSTT1 genotype polymorphisms and rosacea (P=0.003, OR [95% CI]: 4.18 [1.57-11.13]). There were no statistically significant differences between patient and control groups for the GSTP1 Ile/Ile, Ile/Val, and Val/Val genotypes (P>0.05).
We demonstrated a significant association between the GSTT1 and/or GSTM1 null genotypes and rosacea. However, the potential role of GSTs as markers of susceptibility to rosacea needs further studies in larger patient groups.

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    • "Besides the immunological and pathogenic mechanism, recent data suggest that oxidative stress may be an important contributor to the pathogenesis of melanocyte death (Bickers and Athar, 2006; Dell'Anna et al. 2007). The imbalance between oxidative stress and antioxidation seems to induce insufficient antioxidation protection or excess production of reactive oxygen species (ROS) (Passi et al., 1998; Beazley et al., 1999; Jimbow et al., 2001; Boisseau-Garsaud et al., 2002; Rokos et al., 2002; Koca et al., 2004; Ines et al., 2006; Yazici et al., 2006; Arican and Kurutas, 2008). The glutathione S-transferase (GST) superfamily consists of the broadly expressed phase II isoenzymes involved in defense against oxidative stress. "
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