Identification and Herc5-mediated ISGylation of novel target proteins

Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.
Biochemical and Biophysical Research Communications (Impact Factor: 2.28). 10/2006; 348(2):473-7. DOI: 10.1016/j.bbrc.2006.07.076
Source: PubMed

ABSTRACT ISG15, a protein containing two ubiquitin-like domains, is an interferon-stimulated gene product that functions in antiviral response and is conjugated to various cellular proteins (ISGylation) upon interferon stimulation. ISGylation occurs via a pathway similar to the pathway for ubiquitination that requires the sequential action of E1/E2/E3: the E1 (UBE1L), E2 (UbcH8), and E3 (Efp/Herc5) enzymes for ISGylation have been hitherto identified. In this study, we identified six novel candidate target proteins for ISGylation by a proteomic approach. Four candidate target proteins were demonstrated to be ISGylated in UBE1L- and UbcH8-dependent manners, and ISGylation of the respective target proteins was stimulated by Herc5. In addition, Herc5 was capable of binding with the respective target proteins. Thus, these results suggest that Herc5 functions as a general E3 ligase for protein ISGylation.

  • Article: Rgs3
    AfCS-Nature Molecule Pages 01/2009; DOI:10.1038/mp.a002048.01
  • Source
  • [Show abstract] [Hide abstract]
    ABSTRACT: Type I interferons (IFNs) exert their effects through the induction of hundreds of IFN-stimulated genes (ISGs), many of which function by inhibiting viral replication and modulating immune responses. ISG15, a di-ubiquitin-like protein, is one of the most abundantly induced ISGs and is critical for control of certain viral and bacterial infections. Like ubiquitin, ISG15 is covalently conjugated to target proteins. In addition, free unconjugated ISG15 is present both intra- and extracellularly. Although much remains to be learned about conjugated ISG15, even less is known about the 2 free forms of ISG15. This article focuses on the role that ISG15 plays during the host response to pathogen challenge, in particular on the recent observations describing the immunomodulatory properties of free ISG15 and its potential implication in disease pathogenesis.
    Journal of interferon & cytokine research: the official journal of the International Society for Interferon and Cytokine Research 09/2013; DOI:10.1089/jir.2013.0064 · 3.90 Impact Factor

Full-text (2 Sources)

Available from
May 19, 2014