Functions of Vitamin D, Retinoic Acid, and Dexamethasone in Mouse Adipose-Derived Mesenchymal Cells

Children's Surgical Research Program, Department of Surgery, Stanford University School of Medicine, Stanford, California 94305, USA.
Tissue Engineering (Impact Factor: 4.25). 08/2006; 12(7):2031-40. DOI: 10.1089/ten.2006.12.2031
Source: PubMed

ABSTRACT Adipose-derived mesenchymal cells (AMCs) offer great promise for tissue engineering of bone. Previously, 1,25-dihydroxyvitamin D3, retinoic acid (RA), and dexamethasone had been shown to promote osteogenesis in bone marrow-derived mesenchymal cells (BMSCs). To study the osteogenic characteristics of mouse AMCs, we applied these 3 hormones alone and in combination to the AMCs and examined markers of osteogenic differentiation. Interestingly, vitamin D and RA demonstrated a consistent, dose-dependent enhancement of osteogenesis and upregulated osteoblast specific markers including osteopontin and osteocalcin. However, in AMCs, dexamethasone clearly inhibited osteogenic differentiation in a dose dependent fashion and greatly increased the adipogenic marker peroxisome proliferator activated receptor gamma (PPAgamma). In summary, we show in vitro that vitamin D and RA are potential candidates to serve as enhancers of osteogenesis of AMCs and may be incorporated into future cell-based strategies for bone tissue engineering.

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