Article

mig-5/Dsh controls cell fate determination and cell migration in C. elegans

Laboratory of Genetics, University of Wisconsin-Madison, 1117 W. Johnson St., Madison, WI 53706, USA.
Developmental Biology (Impact Factor: 3.64). 11/2006; 298(2):485-97. DOI: 10.1016/j.ydbio.2006.06.053
Source: PubMed

ABSTRACT Cell fate determination and cell migration are two essential events in the development of an organism. We identify mig-5, a Dishevelled family member, as a gene that regulates several cell fate decisions and cell migrations that are important during C. elegans embryonic and larval development. In offspring from mig-5 mutants, cell migrations are defective during hypodermal morphogenesis, QL neuroblast migration, and the gonad arm migration led by the distal tip cells (DTCs). In addition to abnormal migration, DTC fate is affected, resulting in either an absent or an extra DTC. The cell fates of the anchor cell in hermaphrodites and the linker cells in the male gonad are also defective, often resulting in the cells adopting the fates of their sister lineage. Moreover, 2 degrees vulval precursor cells occasionally adopt the 3 degrees vulval cell fate, resulting in a deformed vulva, and the P12 hypodermal precursor often differentiates into a second P11 cell. These defects demonstrate that MIG-5 is essential in determining proper cell fate and cell migration throughout C. elegans development.

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    • "similar to that published for mig-5 alleles (Walston et al., 2006). Simultaneous removal of the function of both dsh-2 and mig-5 by RNAi results in lethality during mid-gastrulation (Walston et al., 2004; our unpublished observations), precluding analysis of later requirements . "
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    • "In the first larval stage (L1), the left Q neuroblast (QL) and its descendents migrate posteriorly, while the right Q neuroblast (QR) and its descendents migrate anteriorly (Figure 1A; Sulston and Horvitz, 1977). Loss of the Wnt EGL-20, the Frizzled receptors MIG-1 or LIN-17, the Dishevelled MIG-5, the b-catenin BAR-1, or the TCF transcription factor POP-1 cause the QL descendents to migrate anteriorly (Harris et al., 1996; Herman, 2001; Maloof et al., 1999; Walston et al., 2006). "
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    • "A functional mig-5::gfp fusion protein was expressed in the B cell and its daughters (Wu and Herman, 2006; Walston et al., 2006). Prior to the B cell division, MIG-5::GFP accumulated asymmetrically as puncta at the anterior cortex or membrane of the B cell in nine out of 13 males (Fig. 4A). "
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