Characterization of the porcine alpha interferon multigene family
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 220 Handan Road, Shanghai 200433, PR China. Gene
(Impact Factor: 2.14).
12/2006; 382:28-38. DOI: 10.1016/j.gene.2006.06.013
The availability of data on the pig genome sequence prompted us to characterize the porcine IFN-alpha (PoIFN-alpha) multigene family. Fourteen functional PoIFN-alpha genes and two PoIFN-alpha pseudogenes were detected in the porcine genome. Multiple sequence alignment revealed a C-terminal deletion of eight residues in six subtypes. A phylogenetic tree of the porcine IFN-alpha gene family defined the evolutionary relationship of the various subtypes. In addition, analysis of the evolutionary rate and the effect of positive selection suggested that the C-terminal deletion is a strategy for preservation in the genome. Eight PoIFN-alpha subtypes were isolated from the porcine liver genome and expressed in BHK-21 cells line. We detected the level of transcription by real-time quantitative RT-PCR analysis. The antiviral activities of the products were determined by WISH cells/Vesicular Stomatitis Virus (VSV) and PK 15 cells/Pseudorabies Virus (PRV) respectively. We found the antiviral activities of intact PoIFN-alpha genes are approximately 2-50 times higher than those of the subtypes with C-terminal deletions in WISH cells and 15-55 times higher in PK 15 cells. There was no obvious difference between the subtypes with and without C-terminal deletion on acid susceptibility.
Available from: Fayna Diaz-San Segundo
- "Similar to other mammals, the swine genome encodes for several IFN genes (Cheng et al., 2006; Sang et al., 2010). The antiviral effect of 18 swine IFNα genes was assessed in CSFV-infected cultures. "
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• Swine interferon alpha is able to alter CSFV pathogenesis.
• Adenovirus expressing swine interferon as a potential biotherapeutic for CSFV.
• Different swine interferon alpha genes possess differential effects on CSFV infection.
Interferon-alpha (IFNα) can effectively inhibit or abort a viral infection within the host. It has been reported that IFN induction and production is hindered during classical swine fever virus (CSFV) infection. Most of those studies have been performed in vitro, making it difficult to elucidate the actual role of IFNs during CSFV infection in swine. Here, we report the effect of IFNα treatment (delivered by a replication defective recombinant human adenovirus type 5, Ad5) in swine experimentally infected with highly virulent CSFV strain Brescia. Treatment with two different subtypes of IFNα delayed the appearance of CSF-related clinical signs and virus replication although it did not prevent lethal disease. This is the first report describing the effect of IFNα treatment during CSFV infection in swine.
Classical swine fever virus; Interferon type I; Protection
Virology 09/2015; 483:284-290. DOI:10.1016/j.virol.2015.04.024 · 3.32 Impact Factor
Available from: Mingwei Xing
- "Interferons (IFNs) represent a large family of functionally related cytokines and they can evoke antiviral (Cheng et al., 2006), antiproliferative , antitumor, and immunomodulatory cellular responses (Zhao et al., 2012). IFNs are commonly divided into three groups: type I, type II and type III. "
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ABSTRACT: Interferon-α (IFN-α) genes have been cloned from a variety of animals, but information regarding crane IFN-α has not been reported to date. In this study, we cloned a full-length Red-crowned Crane interferon-α (crIFN-α) gene sequence consisting of a 486 bp partial 5′ UTR, 741 bp complete ORF and 559 bp partial 3′ UTR. This gene encodes a protein of 246 amino acids and shares 60 to 80% identity with avian IFN-α and less than 45% identity with mammalian IFN-α. The expression of crIFN-α with an N-terminal His-tag was investigated in Escherichia coli, and the protein was purified on a nickel column. To obtain activated proteins, crIFN-α inclusion bodies were renatured by dialysis. In vitro cytopathic inhibition assays indicated that the recombinant crIFN-α could inhibit the replication of vesicular stomatitis virus in chicken fibroblasts. These antiviral activities were abrogated by rabbit anti-crIFN-α antibodies in vitro. In addition, an immunofluorescence assay indicated that crIFN-α could be expressed in chicken fibroblasts and was primarily located in the cytoplasm. Taken together, our results suggest that the crIFN-α gene may play an important role in inhibiting the replication of viruses.
Gene 07/2014; 544(1):49–55. DOI:10.1016/j.gene.2014.04.036 · 2.14 Impact Factor
Available from: Limin Yang
- "The IFN-o shows a high activity against many virus infections and are found in human, pig, cat, and rabbit (Hauptmann and Swetly 1985; Charlier and others 1993; Yang and others 2007; Zhao and others 2009). The type I IFNs have been exploited for the systemic treatment of human HIV, hepatitis B and C infection (Levin and others 1982; Manion and others 2012), but beyond that, type I IFNs play an important role in livestock farming, and IFN-a is well studied in pigs and used as a powerful adjuvant for a recombinant protein vaccine against foot-and-mouth disease virus in swine (Cheng and others 2006, 2007a, 2007b). As to type II and type III IFNs, both have one member each, IFN-g and IFNl , respectively. "
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ABSTRACT: In this study, we provide the first comprehensive annotation of canine interferon-λ (CaIFN-λ, type III IFN). Phylogenetic analysis based on genomic sequences indicated that CaIFN-λ is located in the same branch with Swine IFN-λ1 (SwIFN-λ), Bat IFN-λ1 (BaIFN-λ), and human IFN-λ1 (HuIFN-λ1). CaIFN-λ was cloned, expressed in Escherichia coli, and purified to further investigate the biological activity in vitro. The recombinant CaIFN-λ (rCaIFN-λ) displayed potent antiviral activity on both homologous and heterologous animal cells in terms of inhibiting the replication of the New Jersey serotype of vesicular stomatitis virus (VSV), canine parvovirus, and influenza virus A/WSN/33 (H1N1), respectively. In addition, we also found that rCaIFN-λ exhibits a significant antiproliferative response against A72 canine tumor cells and MDCK cells in a dose-dependent manner. Furthermore, CaIFN-λ activated the JAK-STAT signaling pathway. To evaluate the expression of CaIFN-λ induced by virus and the expression of IFN-stimulated genes (ISGs) induced by rCaIFN-λ in the MDCK cells, we measured the relative mRNA level of CaIFN-λ and ISGs (ISG15, Mx1, and 2'5'-OAS) by quantitative real-time PCR and found that the mRNA level of CaIFN-λ and the ISGs significantly increased after treating the MDCK cells with viruses and rCaIFN-λ protein, respectively. Finally, to evaluate the binding activity of rCaIFN-λ to its receptor, we expressed the extracellular domain of the canine IFN-λ receptor 1 (CaIFN-λR1-EC) and determined the binding activity via ELISA. Our results demonstrated that rCaIFN-λ bound tightly to recombinant CaIFN-λR1-EC (rCaIFN-λR1-EC).
Journal of Interferon & Cytokine Research 06/2014; 34(11). DOI:10.1089/jir.2014.0009 · 2.00 Impact Factor
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