Characterization of the porcine alpha interferon multigene family

State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 220 Handan Road, Shanghai 200433, PR China.
Gene (Impact Factor: 2.14). 12/2006; 382:28-38. DOI: 10.1016/j.gene.2006.06.013
Source: PubMed


The availability of data on the pig genome sequence prompted us to characterize the porcine IFN-alpha (PoIFN-alpha) multigene family. Fourteen functional PoIFN-alpha genes and two PoIFN-alpha pseudogenes were detected in the porcine genome. Multiple sequence alignment revealed a C-terminal deletion of eight residues in six subtypes. A phylogenetic tree of the porcine IFN-alpha gene family defined the evolutionary relationship of the various subtypes. In addition, analysis of the evolutionary rate and the effect of positive selection suggested that the C-terminal deletion is a strategy for preservation in the genome. Eight PoIFN-alpha subtypes were isolated from the porcine liver genome and expressed in BHK-21 cells line. We detected the level of transcription by real-time quantitative RT-PCR analysis. The antiviral activities of the products were determined by WISH cells/Vesicular Stomatitis Virus (VSV) and PK 15 cells/Pseudorabies Virus (PRV) respectively. We found the antiviral activities of intact PoIFN-alpha genes are approximately 2-50 times higher than those of the subtypes with C-terminal deletions in WISH cells and 15-55 times higher in PK 15 cells. There was no obvious difference between the subtypes with and without C-terminal deletion on acid susceptibility.

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    • "The IFN-o shows a high activity against many virus infections and are found in human, pig, cat, and rabbit (Hauptmann and Swetly 1985; Charlier and others 1993; Yang and others 2007; Zhao and others 2009). The type I IFNs have been exploited for the systemic treatment of human HIV, hepatitis B and C infection (Levin and others 1982; Manion and others 2012), but beyond that, type I IFNs play an important role in livestock farming, and IFN-a is well studied in pigs and used as a powerful adjuvant for a recombinant protein vaccine against foot-and-mouth disease virus in swine (Cheng and others 2006, 2007a, 2007b). As to type II and type III IFNs, both have one member each, IFN-g and IFNl , respectively. "
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