Article

Transcriptional profile of the immune response in the lungs of patients with active tuberculosis.

Institute of Neurobiology and Molecular Medicine (INMM), National Research Council, Via del Fosso del Cavaliere, 100, 00133 Rome, Italy.
Clinical Immunology (impact factor: 4.05). 11/2006; 121(1):100-7. DOI:10.1016/j.clim.2006.06.008 pp.100-7
Source: PubMed

ABSTRACT Despite advances in diagnosis and treatment, Mycobacterium tuberculosis causes active disease in about 8 million people worldwide annually. The study of the interplay between the host and the pathogen at the site of infection in human TB may contribute to elucidate the pathogenesis of the disease. In this work, using macroarray technology and real-time PCR, we analyzed the modulation of 847 genes encoding immune-inflammatory mediators in BALF samples of patients affected by active pulmonary TB (PTB) and control patients affected by non-TB diseases. The data show that the PTB milieu contains a complex network of gene activation. Different genes with adhesive properties and involved in tissue repair and fibrosis were modulated. In TB patients, we observed the up-regulation of cytokines, including IFN-gamma and IFN-gamma pathway genes, of several apoptotic genes, and of potent transcriptional activators. These findings can contribute to elucidate the mechanisms of MTB pathogenicity in humans.

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Keywords

8 million people
 
847 genes encoding immune-inflammatory mediators
 
active pulmonary TB
 
adhesive properties
 
apoptotic genes
 
complex network
 
cytokines
 
Different genes
 
gene activation
 
human TB
 
humans
 
IFN-gamma pathway genes
 
macroarray technology
 
MTB pathogenicity
 
Mycobacterium tuberculosis causes active disease
 
non-TB diseases
 
pathogenesis
 
potent transcriptional activators
 
PTB
 
PTB milieu