Construction of recombinant Bacillus subtilis for production of polyhydroxyalkanoates.
ABSTRACT Polyhydroxyalkanoates (PHAs) are polyesters of hydroxyalkanoates synthesized by numerous bacteria as intracellular carbon and energy storage compounds and accumulated as granules in the cytoplasm of cells. In this work, we constructed two recombinant plasmids, pBE2C1 and pBE2C1AB, containing one or two PHA synthse genes, respectively. The two plasmids were inserted into Bacillus subtilis DB104 to generate modified strains, B. subtilis/pBE2C1 and B. subtilis/pBE2C1AB. The two recombinants strains were subjected to fermentation and showed PHA accumulation, the first reported example of mcl-PHA production in B. subtilis. Gas Chromatography analysis identified the compound produced by B. subtilis/pBE2C1 to be a hydroxydecanoate-co-hydroxydodecanoate (HD-co-HDD) polymer whereas that produced by B. subtilis/pBE2C1AB was a hydroxybutyrate-co-hydroxydecanoate-co-hydroxydodecanoate (HB-HD-HDD) polymer.
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Thesis: BIOSYNTHESIS OF POLY(3-HYDROXYBUTYRATE) USING MARINE BACILLUS MEGATERIUM UMTKB-1 By AMIRUL ARIF BIN ABDUL SHUKOR A PITA report submitted in partial fulfilment of the requirements for the award of the degree of Bachelor of Applied Science of Conservation and Management of Biodiversity SCHOOL OF MARINE SCIENCE AND ENVIRONMENT06/2014, Degree: Bachelor of Applied Science of Conservation and Management of Biodiversity, Supervisor: Dr. Kesaven Bhubalan
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ABSTRACT: The kinetics of inactivation of Gram-positive strain, Bacillus subtilis in aquatic systems was investigated as function ozone aeration duration under varied conditions. Oxygen flow was in situ enriched with ozone using ozoniser, with [O(3)] ranging from (0.3 - 9.8) x 10(-5) moles per liter of oxygen. The inactivation kinetics of B. subtilis followed pseudo-first-order kinetics with respect to microbe, under excess [O(3)] conditions. The disinfection kinetics had first order dependence on ozone concentration and the overall second-order rate constant was (7.54 +/- 1.37) x 10(3) M(-1) min(-1). The effect initial temperature and pH of the system on the ozone initiated inactivation of microbe was also explored. Relative to hydroxyl radicals, molecular ozone was found more effective in microbial inactivation. Appropriate mechanism for ozone initiated inactivation is proposed. Ozone aeration significantly decreased the BOD levels of natural and B. subtilis spiked waters.Journal of Environmental Science and Health Part A Toxic/Hazardous Substances & Environmental Engineering 01/2010; 45(2):224-32. DOI:10.1080/10934520903429931
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ABSTRACT: Bacillus, generally regarded as safe, has emerged as a robust organism that can withstand adverse environmental conditions and grow easily to very high densities. Bacillus has been recognized for its biotechnological applications on an industrial scale. Recent efforts have shown the potential of Bacillus to generate biofuels (hydrogen), biopolymers (polyhydroxyalkanoates), and bioactive molecules (acyl-homoserine lactonases). Bacillus can be considered the dark horse in the race to generate sustainable energy, ecofriendly non-fossil fuel-based polymers, and bioactive molecules for use as therapeutics.Biotechnology advances 08/2013; 31(8). DOI:10.1016/j.biotechadv.2013.08.007