Heavy Metal-Induced Differential Gene Expression of Metallothionein in Javanese Medaka, Oryzias javanicus

Southern Coastal Environment Research Division, Korea Ocean Research and Development Institute, Geoje, 656-830, Korea.
Marine Biotechnology (Impact Factor: 3.27). 12/2006; 8(6):654-62. DOI: 10.1007/s10126-006-6046-0
Source: PubMed


A metallothionein (MT) gene was isolated for the first time from Javanese medaka, Oryzias javanicus, which shows high adaptability from freshwater to seawater. The full-length cDNA of MT from O. javanicus (OjaMT) comprises 349 bp, excluding the poly(A)+ stretch, and codes for a total of 60 amino acids. The positions of cysteine residues are highly conserved. The pattern of OjaMT expression induced by six heavy metals was analyzed via real-time quantitative polymerase chain reaction (PCR). The level of hepatic OjaMT mRNA was increased in a dose-dependent manner by Ag, Cd, Cu, and Zn after 24 h of exposure. However, after Cr and Ni exposure, a significant decrease in OjaMT levels was observed. Cadmium-induced OjaMT expression was detectable in fishes as young as 3 months. After Cd exposure, OjaMT induction was prominent in intestine and liver and moderate in muscle and gill. OjaMT mRNA levels could represent a good biomarker for monitoring heavy metals in seawater.

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Available from: Taek-Kyun Lee, Jul 07, 2014
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    • "Java medaka is widely distributed in the coastal areas of Peninsular Malaysia and they are available all year round. It has been utilized in several ecotoxicological studies (Imai et al., 2005, 2007; Ismail and Yusof, 2011; Koyama et al., 2006; Woo et al., 2006; Yu et al., 2006). In this study, we used the early life stages of the fish to investigate their responses after exposure to glyphosate-based herbicide. "
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    ABSTRACT: Glyphosate is globally a widely used herbicide, yet there is little information on their toxicity to marine fishes. Java medaka, a small tropical fish native to coastal areas in several Southeast Asian countries, is viewed as a suitable candidate for toxicity test and thus was used for this study. Java medaka adults were cultured in the laboratory and the fertilized eggs of the F2 generation were exposed to different concentrations of glyphosate-based herbicide (100, 200, 300, 400 and 500ppm) until they hatched. The survival and hatching rates of the embryos, changes in the heart rate and morphological impairments were recorded. Generally, survival and hatching percentage decreased as glyphosate concentration increased. Absence of pectoral fin(s) and cornea, permanently bent tail, irregular shaped abdomen, and cell disruption in the fin, head and abdomen are among the common teratogenic effects observed. Furthermore, risk factor also increased with the increased in glyphosate concentrations.
    Marine Pollution Bulletin 04/2014; 85(2). DOI:10.1016/j.marpolbul.2014.03.022 · 2.99 Impact Factor
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    • "Letters above the bars show the level of statistical difference between the bars radical damage. These data support other data using medaka fish which reveal complex interactions between metals (Tilton et al. 2003; Woo et al. 2006; Barjhoux et al. 2012). Little et al. (2007) looked at cobalt toxicity in combination with UV and found that if the fish were UV adapted, the cobalt–UV combined exposure was relatively less toxic than cobalt alone but if the two exposures were given without prior UV adaptation, then the combination was more toxic than the additive effect should be. "
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    ABSTRACT: Very little is known about the combined effects of low doses of heavy metals and radiation. However, such "multiple stressor" exposure is the reality in the environment. In the work reported in this paper, fish were exposed to cobalt 60 gamma irradiation with or without copper or aluminum in the water. Doses of radiation ranged from 4 to 75 mGy delivered over 48 or 6 h. Copper doses ranged from 10 to 80 μg/L for the same time period. The aluminum dose was 250 μg/L. Gills and skin were removed from the fish after exposure and explanted in tissue culture flasks for investigation of bystander effects of the exposures using a stress signal reporter assay, which has been demonstrated to be a sensitive indicator of homeostatic perturbations in cells. The results show complex synergistic interactions of radiation and copper. Gills on the whole produce more toxic bystander signals than skin, but the additivity scores show highly variable results which depend on dose and time of exposure. The impacts of low doses of copper and low doses of radiation are greater than additive, medium levels of copper alone have a similar level of effect of bystander signal toxicity to the low dose. The addition of radiation stress, however, produces clear protective effects in the reporters treated with skin-derived medium. Gill-derived medium from the same fish did not show protective effects. Radiation exposure in the presence of 80 μg/L led to highly variable results, which due to animal variation were not significantly different from the effect of copper alone. The results are stressor type, stressor concentration and time dependent. Clearly co-exposure to radiation and heavy metals does not always lead to simple additive effects.
    Biophysik 12/2013; DOI:10.1007/s00411-013-0505-6 · 1.53 Impact Factor
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    • "Metallothioneins (MTs) are considered to be essential biomarkers in metal-induced toxicity [38] as facilitating metal detoxification and protection from free radicals [39]. A report on heavy metal toxicity in Javanese medaka showed that MT upregulation occurs in silver mediated toxicity [40]. Hemeoxygenase-1 (HO-1) is an ROS sensor and a cryoprotective agent possessing antioxidant and anti-inflammatory properties. "
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    ABSTRACT: Since silver-nanoparticles (NPs) possess an antibacterial activity, they were commonly used in medical products and devices, food storage materials, cosmetics, various health care products, and industrial products. Various silver-NP based medical devices are available for clinical uses, such as silver-NP based dressing and silver-NP based hydrogel (silver-NP-hydrogel) for medical applications. Although the previous data have suggested silver-NPs induced toxicity in vivo and in vitro, there is lack information about the mechanisms of biological response and potential toxicity of silver-NP-hydrogel. In this study, the genotoxicity of silver-NP-hydrogel was assayed using cytokinesis-block micronucleus (CBMN). The molecular response was studied using DNA microarray and GO pathway analysis. The results of global gene expression analysis in HeLa cells showed that thousands of genes were up- or down-regulated at 48 h of silver-NP-hydrogel exposure. Further GO pathway analysis suggested that fourteen theoretical activating signaling pathways were attributed to up-regulated genes; and three signal pathways were attributed to down-regulated genes. It was discussed that the cells protect themselves against silver NP-mediated toxicity through up-regulating metallothionein genes and anti-oxidative stress genes. The changes in DNA damage, apoptosis and mitosis pathway were closely related to silver-NP-induced cytotoxicity and chromosome damage. The down-regulation of CDC14A via mitosis pathway might play a role in potential genotoxicity induced by silver-NPs. The silver-NP-hydrogel induced micronuclei formation in cellular level and broad spectrum molecular responses in gene expression level. The results of signal pathway analysis suggested that the balances between anti-ROS response and DNA damage, chromosome instability, mitosis inhibition might play important roles in silver-NP induced toxicity. The inflammatory factors were likely involved in silver-NP-hydrogel complex-induced toxic effects via JAK-STAT signal transduction pathway and immune response pathway. These biological responses eventually decide the future of the cells, survival or apoptosis.
    Journal of Nanobiotechnology 05/2012; 10(1):16. DOI:10.1186/1477-3155-10-16 · 4.12 Impact Factor
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