Endocrine Disruptor Vinclozolin Induced Epigenetic Transgenerational Adult-Onset Disease
Washington State University, پولمن، واشینگتن, Washington, United States Endocrinology
(Impact Factor: 4.5).
01/2007; 147(12):5515-23. DOI: 10.1210/en.2006-0640
The fetal basis of adult disease is poorly understood on a molecular level and cannot be solely attributed to genetic mutations or a single etiology. Embryonic exposure to environmental compounds has been shown to promote various disease states or lesions in the first generation (F1). The current study used the endocrine disruptor vinclozolin (antiandrogenic compound) in a transient embryonic exposure at the time of gonadal sex determination in rats. Adult animals from the F1 generation and all subsequent generations examined (F1-F4) developed a number of disease states or tissue abnormalities including prostate disease, kidney disease, immune system abnormalities, testis abnormalities, and tumor development (e.g. breast). In addition, a number of blood abnormalities developed including hypercholesterolemia. The incidence or prevalence of these transgenerational disease states was high and consistent across all generations (F1-F4) and, based on data from a previous study, appears to be due in part to epigenetic alterations in the male germ line. The observations demonstrate that an environmental compound, endocrine disruptor, can induce transgenerational disease states or abnormalities, and this suggests a potential epigenetic etiology and molecular basis of adult onset disease.
Available from: Carlos Guerrero-Bosagna
- "The F1 to F4 generation offspring were not themselves treated directly with DDT. The control and DDT lineages were housed in the same room and racks with lighting, food and water as previously described [1,5,7]. All experimental protocols for the procedures with rats were preapproved by the Washington State University Animal Care and Use Committee (IACUC approval no. "
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ABSTRACT: Ancestral environmental exposures to a variety of environmental factors and toxicants have been shown to promote the epigenetic transgenerational inheritance of adult onset disease. The present work examined the potential transgenerational actions of the insecticide dichlorodiphenyltrichloroethane (DDT) on obesity and associated disease.
Outbred gestating female rats were transiently exposed to a vehicle control or DDT and the F1 generation offspring bred to generate the F2 generation and F2 generation bred to generate the F3 generation. The F1 and F3 generation control and DDT lineage rats were aged and various pathologies investigated. The F3 generation male sperm were collected to investigate methylation between the control and DDT lineage male sperm.
The F1 generation offspring (directly exposed as a fetus) derived from the F0 generation exposed gestating female rats were not found to develop obesity. The F1 generation DDT lineage animals did develop kidney disease, prostate disease, ovary disease and tumor development as adults. Interestingly, the F3 generation (great grand-offspring) had over 50% of males and females develop obesity. Several transgenerational diseases previously shown to be associated with metabolic syndrome and obesity were observed in the testis, ovary and kidney. The transgenerational transmission of disease was through both female (egg) and male (sperm) germlines. F3 generation sperm epimutations, differential DNA methylation regions (DMR), induced by DDT were identified. A number of the genes associated with the DMR have previously been shown to be associated with obesity.
Observations indicate ancestral exposure to DDT can promote obesity and associated disease transgenerationally. The etiology of disease such as obesity may be in part due to environmentally induced epigenetic transgenerational inheritance.
BMC Medicine 10/2013; 11(1):228. DOI:10.1186/1741-7015-11-228 · 7.25 Impact Factor
Available from: Susan E Ozanne
- "A pattern of down-regulation at key methylation sites in the testes was also observed through to the F3 generation (Anway et al., 2008). The offspring phenotype in rat males following initial F0 vinclozolin exposure persisted to the F4 generation (Anway et al., 2006a, b), and in females to the F3 generation (Nilsson et al., 2008), although these findings have been disputed subsequently (Inawaka et al., 2009). The phenotype included excess tumours and disrupted kidney development in both the male and female rat offspring (Anway et al., 2008; Nilsson et al., 2008). "
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The concept of developmental programming suggests that the early life environment influences offspring characteristics in later life, including the propensity to develop diseases such as the metabolic syndrome. There is now growing evidence that the effects of developmental programming may also manifest in further generations without further suboptimal exposure. This review considers the evidence, primarily from rodent models, for effects persisting to subsequent generations, and evaluates the mechanisms by which developmental programming may be transmitted to further generations. In particular, we focus on the potential role of the intrauterine environment in contributing to a developmentally programmed phenotype in subsequent generations.METHODS
The literature was systematically searched at http://pubmed.org and http://scholar.google.com to identify published findings regarding transgenerational (F2 and beyond) developmental programming effects in human populations and animal models.RESULTSTransmission of programming effects is often viewed as a form of epigenetic inheritance, either via the maternal or paternal line. Evidence exists for both germline and somatic inheritance of epigenetic modifications which may be responsible for phenotypic changes in further generations. However, there is increasing evidence for the role of both extra-genomic components of the zygote and the interaction of the developing conceptus with the intrauterine environment in propagating programming effects.CONCLUSIONS
The contribution of a suboptimal reproductive tract environment or maternal adaptations to pregnancy may be critical to inheritance of programming effects via the maternal line. As the effects of age exacerbate the programmed metabolic phenotype, advancing maternal age may increase the likelihood of developmental programming effects being transmitted to further generations. We suggest that developmental programming effects could be propagated through the maternal line de novo in generations beyond F2 as a consequence of development in a suboptimally developed intrauterine tract and not necessarily though directly transmitted epigenetic mechanisms.
Human Reproduction Update 09/2013; 20(1). DOI:10.1093/humupd/dmt043 · 10.17 Impact Factor
Available from: Barbara Bilinska
- "Several studies indicate that upstream mechanisms leading to the long-term alterations observed in adult prostate may be related to the epigenetic changes, such as DNA methylation and histone modifications. It was reported that changes in methylation states of several genes induced by exposure to antiandrogen vinclozolin during the period of sex determination are correlated with disease state in numerous organs of adult rats, including prostate [5, 59]. The role of epigenetic factors was also demonstrated by Ho et al. , who found altered methylation pattern of phosphodiesterase type 4 in adult rat prostate following neonatal treatment with estradiol. "
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ABSTRACT: To date, limited knowledge exists regarding the role of the androgen signaling during specific periods of development in the regulation of androgen receptor (AR) and connexin 43 (Cx43) in adult prostate. Therefore, in this study we examined mRNA and protein expression, and tissue distribution of AR and Cx43 in adult boar prostates following fetal (GD20), neonatal (PD2), and prepubertal (PD90) exposure to an antiandrogen flutamide (50 mg/kg bw). In GD20 and PD2 males we found the reduction of the luminal compartment, inflammatory changes, decreased AR and increased Cx43 expression, and altered localization of both proteins. Moreover, enhanced apoptosis and reduced proliferation were detected in the prostates of these animals. In PD90 males the alterations were less evident, except that Cx43 expression was markedly upregulated. The results presented herein indicate that in boar androgen action during early fetal and neonatal periods plays a key role in the maintenance of normal phenotype and functions of prostatic cells at adulthood. Furthermore, we demonstrated that modulation of Cx43 expression in the prostate could serve as a sensitive marker of hormonal disruption during different developmental stages.
09/2013; 2013(2):407678. DOI:10.1155/2013/407678
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