Detection and phylogenic analysis of one anthrax virulence plasmid pXO1 conservative open reading frame ubiquitous presented within Bacillus cereus group strains.

Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, China.
Biochemical and Biophysical Research Communications (Impact Factor: 2.3). 12/2006; 349(4):1214-9. DOI: 10.1016/j.bbrc.2006.08.125
Source: PubMed

ABSTRACT The presence of one of the anthrax virulence plasmid pXO1 conserved fragments was analyzed in 24 Bacillus cereus and B. thuringiensis strains, including 6 B. thuringiensis subspecies, by polymerase chain reactions. Twelve out of 24 strains showed PCR-positive for an ORF101 homologous sequence. Two pXO1-ORF101-like fragments from a B. cereus B-4ac and a commercial B. thuringiensis kurstaki HD1 were cloned, sequenced and expressed in Escherichia coli. Toxicity assays revealed that the product encoded by the pXO1-ORF101-like fragment had no impact on either Vero cells or Chinese Hamster Ovary cells, suggesting that this fragment probably not contribute to enterotoxic activity. Sequence alignment of the pXO1-ORF101 from three Bacillus anthracis and ORF101-like fragments from other 12 B. cereus group isolates indicated high identity (more than 90%) and the presence of subgroup- and strain-specific SNPs among these fragments.

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Available from: Zhi-ming Yuan, Jun 15, 2015
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    • "An assay that accurately distinguishes between Bacillus anthracis and other members of the Bacillus spp. would prove very useful (Helgason et al., 2000; Hu et al., 2006; Turnbull, 1999). Differentiation of B. anthracis from its close relatives has traditionally relied upon phenotypic characterisation (Dixon et al., 1999), and unambiguous identification requires multiple biochemical tests (Brown et al., 1958; Makino et al., 1993; Muller et al., 2004; Turnbull, 1999), usually taking 1–2 days and requiring referral to more specialised laboratories. "
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