Involvement of TNFα –308A Promoter Polymorphism in the Development of Asthma in Children Infected With Chlamydophila pneumoniae

Department of Genetics, Cell and Immunobiology, Semmelweis University, H-1089, Budapest, Hungary.
Pediatric Research (Impact Factor: 2.31). 12/2006; 60(5):543-8. DOI: 10.1203/01.pdr.0000242298.24089.52
Source: PubMed


Several data indicate a connection between Chlamydophila pneumoniae infection and asthma. Although C. pneumoniae is a common cause of infection, not all infected patients develop asthma. This suggests that certain individuals may be genetically predisposed to the chronic effects of C. pneumoniae infection on airway functions. We investigated the possible modifying effect of different polymorphisms on C. pneumoniae infection and on the susceptibility to asthma in 318 children, among those 144 had asthma and 174 had no asthmatic symptoms. C. pneumoniae-specific antibodies were measured by ELISA. Tumor necrosis factor-alpha (TNFalpha), monocyte chemoattractant protein-1 (MCP-1), and RANTES (regulated on activation normal T cell expressed and secreted) genotypes were determined by PCR-restriction fragment length polymorphism (RFLP). There were no significant differences in the percentage of children positive for C. pneumoniae-specific antibodies between cases and controls. None of the genotypes was associated with altered susceptibility to C. pneumoniae infection. Among asthmatic children carrying the TNFalpha -308A allele, there were significantly more patients positive for C. pneumoniae-specific IgG, than among control children carrying the same allele (20.1% versus 9.2% of asthmatic versus control children, respectively; p = 0.002; odds ratio = 3.52 (1.52-7.53); p = 0.005). This study indicates the possible roles of polymorphisms in the immune system in the susceptibility to asthma in children infected with C. pneumoniae.

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    • "Several reports found associations between the –308A allele and asthma. An example for the gene–environmental interaction in asthma is, that children positive for Chlamydophila pneumoniae-specific IgG carrying the TNF À308A allele have considerably higher risk of developing asthma than children with similar infection status carrying wild-type genotypes (Tölgyesi et al., 2006). Nicolae et al. (2005) conducted a genome-wide screen of families who participated in the Collaborative Study on the Genetics of Asthma. "
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