Article

Hairy roots induced by Agrobacterium rhizogenes and production of regenerative plants in hairy root cultures in maize.

School of Life Sciences, Northeast Normal University, Changchun 130024, China.
Science in China Series C Life Sciences (impact factor: 1.61). 09/2006; 49(4):305-10. pp.305-10
Source: PubMed

ABSTRACT Hairy roots of maize were induced by infecting 15-d calli with Agrobacterium rhizogenes. The hairy roots cultured in hormone-free media showed the vigorous growth and typical hairy root features. The regenerated plants were produced from hairy roots in MS media supplemented with 1.6 mg/L ZT and 0.4 mg/L NAA. The PCR-Southern hybridization demonstrated that T-DNA had been integrated into the chromosome of regenerated plants.

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Keywords

Agrobacterium rhizogenes
 
hairy roots
 
hairy roots cultured
 
hormone-free media
 
infecting 15-d calli
 
MS media supplemented
 
PCR-Southern hybridization
 
typical hairy
 
vigorous growth
 

Hongwei Xu