Törjék O, Witucka-Wall H, Meyer RC, Korff M, Kusterer B, Rautengarten C et al.. Segregation distortion in Arabidopsis C24/Col-0 and Col-0/C24 recombinant inbred line populations is due to reduced fertility caused by epistatic interaction of two loci. Theor Appl Genet 113: 1551-1561

Department of Genetics, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476, Potsdam-Golm, Germany.
Theoretical and Applied Genetics (Impact Factor: 3.79). 12/2006; 113(8):1551-61. DOI: 10.1007/s00122-006-0402-3
Source: PubMed


A new large set of reciprocal recombinant inbred lines (RILs) was created between the Arabidopsis accessions Col-0 and C24 for quantitative trait mapping approaches, consisting of 209 Col-0 x C24 and 214 C24 x Col-0 F(7 )RI lines. Genotyping was performed using 110 evenly distributed framework single nucleotide polymorphism markers, yielding a genetic map of 425.70 cM, with an average interval of 3.87 cM. Segregation distortion (SD) was observed in several genomic regions during the construction of the genetic map. Linkage disequilibrium analysis revealed an association between a distorted region at the bottom of chromosome V and a non-distorted region on chromosome IV. A detailed analysis of the RILs for these two regions showed that an SD occurred when homozygous Col-0 alleles on chromosome IV coincided with homozygous C24 alleles at the bottom of chromosome V. Using nearly isogenic lines segregating for the distorted region we confirmed that this genotypic composition leads to reduced fertility and fitness.

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Available from: Carsten Rautengarten, Jun 05, 2015
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    • "Segregation distortion is a common fact in segregated populations generated from crosses between diverse genotypes [64,65]. In plants, segregation distortion was first reported in maize [66], and subsequently in many species including rice [67], wheat [68], Arabidopsis [69], and cabbage [14]. Segregation distortion can have important implications for the construction of a genetic map and QTL mapping, but if addressed properly, distorted markers can also be helpful for QTL mapping [70]. "
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    • "and do not indicate substantial two - locus linkage disequilibrium as has been previously described in the progeny from some A . thaliana crosses ( Törjék et al . , 2006 ; Simon et al . , 2008 ) . QTL detection was performed using the R / qtl package ( Broman et al . , 2003"
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