Comparative study of monoclonal antibody B72.3 and gross cystic disease fluid protein-15 as markers of apocrine carcinoma of the breast

Research Team for Geriatric Diseases, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.
Apmis (Impact Factor: 2.04). 11/2006; 114(10):712-9. DOI: 10.1111/j.1600-0463.2006.apm_434.x
Source: PubMed


Gross cystic disease fluid protein-15 (GCDFP-15) is a commonly used apocrine marker; however, its expression was recently found to decrease in infiltrating, larger, or metastasizing apocrine carcinomas of the breast. In the breast, monoclonal antibody (MAb) B72.3 has been reported to be useful as an apocrine marker although it is used for that purpose much less frequently than GCDFP-15. In the search for a more consistent apocrine marker, immunoreactivity for MAb B72.3 was examined in apocrine carcinomas at different stages and compared with GCDFP-15. 47 of 51 apocrine carcinomas (92%) and 9 of 62 ordinary carcinomas (15%) were MAb B72.3 positive, while 39 of 51 apocrine carcinomas (76%) and 13 of 62 ordinary carcinomas (21%) were GCDFP-15 positive. Thus, both sensitivity and specificity were higher for MAb B72.3. Furthermore, unlike GCDFP-15, MAb B72.3 exhibited positivity irrespective of infiltrating status, tumor size, or metastatic status. There was no correlation between MAb B72.3-immunoreactivity and GCDFP-15-expression. The combined usage of MAb B72.3 with GCDFP-15 was useful to confirm the diagnosis of apocrine carcinoma, especially for advanced tumors, with only two cases being negative for both MAb B72.3 and GCDFP-15. Whether these two cases should be differentiated from ordinary apocrine carcinomas remains to be investigated.

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    • "Its expression has been observed in approximately 60% of apocrine carcinomas and correlated with adverse parameters, including angiolympathic invasion, higher histologic grade, and poor clinical outcome (Kasashima et al., 2012). Other markers that have been proposed to be specific for apocrine differentiation include gama-glutaminyl transferase 1 (GGT-1) (Banneau et al., 2010; Choi et al., 2012; Kim et al., 2012; Cha et al., 2012) and tumorassociated glycoprotein-72, a high molecular weight glycoprotein that appears to be a more sensitive marker of apocrine differentiation than GCDFP-15 (Honma et al., 2006). "
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    • "The absence of positive oestrogen and progesterone receptors in patient 1's gastric biopsies led to the initial assumption that this was a primary gastric cancer. Positive monoclonal staining with GCDFP-15 (gross cystic disease fluid protein-15) has been found to be a sensitive (55–76%) and specific (95–100%) marker to correctly identify a malignant lesion as metastatic breast carcinoma [5,16-21]. This marker is a monoclonal antibody of gross cystic disease fluid protein-15 (GCDFP-15) which is detected in macroscopic breast cyst fluid and in the plasma of patients with breast cancer [22,23]. "
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