Article

Clonal analysis of mouse development reveals a polyclonal origin for yolk sac blood islands.

Institute of Stem Cell Biology and Regenerative Medicine and Department of Pathology and Developmental Biology, Stanford University School of Medicine, Stanford, California 94305, USA.
Developmental Cell (Impact Factor: 10.37). 11/2006; 11(4):519-33. DOI: 10.1016/j.devcel.2006.08.001
Source: PubMed

ABSTRACT Direct clonal analysis of tissue and organ maturation in vivo is a critical step in the interpretation of in vitro cell precursor-progeny relationships. We have developed a method to analyze clonal progenitor contributions in vivo using ES cells stably expressing separate fluorescent proteins and placed into normal blastocysts to form tetrachimeras. Here we applied this method to the analysis of embryonic yolk sac blood islands. In most vertebrates, yolk sac blood islands are the initial sites of appearance of hematopoietic and endothelial cells. It has been proposed that these lineages arise from a common clonal progenitor, the hemangioblast, but this hypothesis has not been tested directly in physiological development in vivo. Our analysis shows that each island has contributions from multiple progenitors. Moreover, contribution by individual hemangioblast progenitors to both endothelial and hematopoietic lineages within an island, if it happens at all, is an infrequent event.

0 Followers
 · 
69 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Tissue Engineering and regenerative is aiming at generating tissues to replace damaged and deteriorated organs. Recently, tissue engineering was applied to generate artificial skin for burn patients, tissue engineered trachea, cartilage for knee-replacement procedures, urinary bladder, urethra substitutes and offered cellular therapies for the treatment of urinary incontinence. The major advantage of tissue engineering approach over traditional organ transplantation is to circumvent the problem of organ shortage. Tissues reconstructed from readily available patents' stem cells induced no immunogenicity when reimplanted in the patient. However, pluripotent stem cells are major limited factors in regenerating new tissues. To overcome these problems, we developed a new technology called as “invivo interspecies tissue engineering” (INVITE) and used the potential of induced pluripotent stem cells (iPSCs) to regenerate new tissues in a host organism. As a model, we used the mouse pluripotent stem cells to assess the potential of these cells to regenerate mouse tissues in rat. Three chimeric rats have been generated by mouse induced pluripotent stem cells assessed by monitoring of green fluorescence protein (GFP) and polymerase chain reaction (PCR) assays. This study shows clearly that mouse induced pluripotent stem cells are able to engraft in rat embryos and are capable to differentiate to multiple tissues. Beside the enormous important application of in vivo tissue engineering in human to develop new therapeutic approaches through regenerating new tissues, this model offers an accessible system for study of organ development and a valuable tool for personalized drug screening and a novel approach for personalized stem cell-based tissue regeneration.
    09/2014; DOI:10.1002/cbi3.10018
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: How are skeletal tissues derived from skeletal stem cells? Here, we map bone, cartilage, and stromal development from a population of highly pure, postnatal skeletal stem cells (mouse skeletal stem cells, mSSCs) to their downstream progenitors of bone, cartilage, and stromal tissue. We then investigated the transcriptome of the stem/progenitor cells for unique gene-expression patterns that would indicate potential regulators of mSSC lineage commitment. We demonstrate that mSSC niche factors can be potent inducers of osteogenesis, and several specific combinations of recombinant mSSC niche factors can activate mSSC genetic programs in situ, even in nonskeletal tissues, resulting in de novo formation of cartilage or bone and bone marrow stroma. Inducing mSSC formation with soluble factors and subsequently regulating the mSSC niche to specify its differentiation toward bone, cartilage, or stromal cells could represent a paradigm shift in the therapeutic regeneration of skeletal tissues. Copyright © 2015 Elsevier Inc. All rights reserved.
  • Source

Full-text (2 Sources)

Download
36 Downloads
Available from
Jun 5, 2014