Article

Rev-erbalpha upregulates NF-kappaB-responsive genes in vascular smooth muscle cells.

Cardiovascular Research, Drug Discovery Institute, Nihon Schering K.K., 1900-1, Togo Mobara, Chiba 297-0017, Japan.
FEBS Letters (impact factor: 3.54). 04/2004; 561(1-3):69-74. DOI:10.1016/S0014-5793(04)00118-8 pp.69-74
Source: PubMed

ABSTRACT Rev-erbalpha and retinoic acid receptor-related orphan receptor-alpha (RORalpha) are orphan nuclear receptors but their effects on transcription are opposed. Here, we show that Rev-erbalpha was expressed predominantly in vascular smooth muscle cells (VSMCs) rather than endothelial cells. Overexpression of Rev-erbalpha upregulated the expression of interleukin-6 and cyclooxygenase-2, and increased transactivation by NF-kappaB and translocation of p65 to the nucleus in A7r5 VSMCs. Furthermore, the expression of Rev-erbalpha was upregulated by RORalpha1 but that upregulation was attenuated by Rev-erbalpha itself in A7r5 VSMCs. These results suggest a regulatory link between Rev-erbalpha and the NF-kappaB pathway.

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Keywords

A7r5 VSMCs
 
cyclooxygenase-2
 
interleukin-6
 
NF-kappaB
 
NF-kappaB pathway
 
nucleus
 
Overexpression
 
regulatory link
 
retinoic acid receptor-related orphan receptor-alpha
 
Rev-erbalpha
 
Rev-erbalpha upregulated
 
RORalpha1
 
transactivation
 
transcription
 
vascular smooth muscle cells
 
VSMCs
 

Hideyuki Migita