Growth factors and cytokines in autologous platelet concentrate and their correlation to periodontal regeneration outcomes. J Clin Periodontol

Department of Operative Dentistry and Periodontology, University of Regensburg, Regensburg, Germany.
Journal Of Clinical Periodontology (Impact Factor: 4.01). 11/2006; 33(11):837-45. DOI: 10.1111/j.1600-051X.2006.00991.x
Source: PubMed


To determine the concentration of naturally available biologic mediators in autologous platelet concentrates and their correlation with periodontal regeneration outcomes.
In 25 patients with two intra-bony defects each, an autologous platelet concentrate (APC) was prepared by a laboratory thrombocyte apheresis technique pre-operatively. Both defects were treated using a bioresorbable guided tissue regeneration-membrane in combination with tricalciumphosphate (TCP). In the test defect, APC was additionally applied. In the APC, platelets were counted and the levels of growth factors and cytokines were determined by ELISA. Correlations between the platelet counts or the growth factor/cytokine levels and the potential clinical and radiographic regeneration outcomes due to APC were calculated after 3, 6, and 12 months.
The APC contained 2.2 x 10(6) platelets/mul, which was 7.9 times more than in the venous blood. Transforming growth factor-beta1 (TGF-beta1), insulin-like growth factor-I (IGF-I), platelet-derived growth factor-AB (PDGF-AB), PDGF-BB, vascular endothelial growth factor (VEGF), and epidermal growth factor (EGF) were found in the APC, whereas interleukin-1beta (IL-1beta), IL-6, tumor necrosis factor alpha (TNFalpha), IL-4, and IL-10 were not detectable. The regression analysis showed a weak correlation between the platelet counts or the growth factor levels and the clinical and radiographic regeneration outcomes (r2<or=0.4).
Autologous platelet concentrate contains relatively high concentrations of PDGF-AB, PDGF-BB, TGF-beta1, and IGF-I, but their potential influence on periodontal regeneration remains unclear.

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    • "Determination of the optimal dose of PRCR leads to definition of the appropriate levels of the main growth factors that are responsible for cells proliferation and maturation. For example, TGF-β1, a growth factor detected at high concentrations in PRCR, has been identified as an important inducer of the myofibroblastic phenotype 24-26. Human skin fibroblasts generally show significant expression of myofibroblast marker (α-SMA) after the treatment with PRCR. "
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    ABSTRACT: Platelet rich plasma clot- releasate (PRCR) shows significant influence on tissue regeneration in clinical trials. Although, the mechanism of PRCR effect on fibroblast differentiation has been studied on 2D culture system, a detailed investigation is needed to establish the role of PRCR in cell seeded in 3D scaffolds. Therefore, a study was conducted to evaluate the influence of PRCR in fibroblasts (DFB) differentiation and extracellular matrix formation on both 3D and 2D culture systems. Cell viability was measured using MTT assay and DFB differentiation was evaluated by determining the expression levels of nucleostamin and alpha smooth muscle actin (α-SMA), using indirect immunostaining and Western blotting. The expression levels of extracellular matrix genes (collagen-I, collagen-III, fibronectin and laminin) and focal adhesion formation gene (integrin beta-1) were measured using Real-time PCR. The PRCR at 10% showed significant effect on cells viability compared with 5% and 20% in both culture environments. The decrease in the expression levels of nucleostamin and the increase in α-SMA signify the DFB differentiation to myofibroblast-like cells that was prominently greater in 3D compared to 2D culture. In 3D culture systems, the total collage production, expression levels of the extracellular matrix gene and the focal adhesion gene were increased significantly compared to 2D culture. In conclusion, 3D culture environments enhances the proliferative and differentiation effects of PRCR on DFB, thereby potentially increases the efficacy of DFB for future tissue engineering clinical application.
    International journal of medical sciences 06/2014; 11(10). DOI:10.7150/ijms.8895 · 2.00 Impact Factor
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    • "Se mantuvo la constante de que las mayores concentraciones de PDGFBB se presentaron en el PRP tanto antes como después del tratamiento, además los niveles promedio de PDGFBB antes del tratamiento fueron similares a los reportados por otros investigadores cuyos valores oscilaron entre 2,3 y 37 ng/ml. Entre dichos estudios, vale la pena mencionar el realizado por Christgau M y col (2006), quienes evaluaron los niveles de FC y citocinas en concentrados plaquetarios de donantes de sangre estableciendo su correlación con la regeneración periodontal. En este estudio los niveles de PDGFBB se situaron en 15,8 ± 7,9 ng/ml, considerándose dichos niveles elevados (30, 31, 35, 43, 44). "
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    ABSTRACT: Niveles del factor de crecimiento derivado de plaquetas en el plasma rico en plaquetas antes y despues de antiagregantes plaquetarios (PDGF levels in platelet-rich plasma before and after anti platelets drugs)
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    • "Normally, bFGF is produced primarily by periodontal ligament fibroblasts and endothelial cells in the periodontal tissue. In the healing stage of periodontitis, the basic cellular mechanisms of periodontal regeneration are similar to wound healing and involve proliferation and migration of fibroblasts in the periodontal ligament, the differentiation of osteoblasts and cementoblasts from undifferentiated precursor cells, and ultimately, the synthesis of extracellular matrix [43, 44]. bFGF has been shown to be effective in promoting periodontal regeneration in a series of studies [10, 11, 16, 17], whereas the role bFGF plays in the development and recovery stage of periodontitis healing still keeps unknown. "
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