Article

Growth factors and cytokines in autologous platelet concentrate and their correlation to periodontal regeneration outcomes. J Clin Periodontol

Department of Operative Dentistry and Periodontology, University of Regensburg, Regensburg, Germany.
Journal Of Clinical Periodontology (Impact Factor: 3.61). 11/2006; 33(11):837-45. DOI: 10.1111/j.1600-051X.2006.00991.x
Source: PubMed

ABSTRACT To determine the concentration of naturally available biologic mediators in autologous platelet concentrates and their correlation with periodontal regeneration outcomes.
In 25 patients with two intra-bony defects each, an autologous platelet concentrate (APC) was prepared by a laboratory thrombocyte apheresis technique pre-operatively. Both defects were treated using a bioresorbable guided tissue regeneration-membrane in combination with tricalciumphosphate (TCP). In the test defect, APC was additionally applied. In the APC, platelets were counted and the levels of growth factors and cytokines were determined by ELISA. Correlations between the platelet counts or the growth factor/cytokine levels and the potential clinical and radiographic regeneration outcomes due to APC were calculated after 3, 6, and 12 months.
The APC contained 2.2 x 10(6) platelets/mul, which was 7.9 times more than in the venous blood. Transforming growth factor-beta1 (TGF-beta1), insulin-like growth factor-I (IGF-I), platelet-derived growth factor-AB (PDGF-AB), PDGF-BB, vascular endothelial growth factor (VEGF), and epidermal growth factor (EGF) were found in the APC, whereas interleukin-1beta (IL-1beta), IL-6, tumor necrosis factor alpha (TNFalpha), IL-4, and IL-10 were not detectable. The regression analysis showed a weak correlation between the platelet counts or the growth factor levels and the clinical and radiographic regeneration outcomes (r2<or=0.4).
Autologous platelet concentrate contains relatively high concentrations of PDGF-AB, PDGF-BB, TGF-beta1, and IGF-I, but their potential influence on periodontal regeneration remains unclear.

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    • "Se mantuvo la constante de que las mayores concentraciones de PDGFBB se presentaron en el PRP tanto antes como después del tratamiento, además los niveles promedio de PDGFBB antes del tratamiento fueron similares a los reportados por otros investigadores cuyos valores oscilaron entre 2,3 y 37 ng/ml. Entre dichos estudios, vale la pena mencionar el realizado por Christgau M y col (2006), quienes evaluaron los niveles de FC y citocinas en concentrados plaquetarios de donantes de sangre estableciendo su correlación con la regeneración periodontal. En este estudio los niveles de PDGFBB se situaron en 15,8 ± 7,9 ng/ml, considerándose dichos niveles elevados (30, 31, 35, 43, 44). "
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    • "The following GF were evaluated: platelet derived growth factor-AB and -BB (PDGF-AB, PDGF-BB), transforming growth factor-b1 and -b2 (TGF-b1, TGF-b2), epidermal growth factor (EGF), insulin-like growth factor-I (IGF-I), basic-fibroblast growth factor (b-FGF) and vascular endothelial growth factor (VEGF) [20] [21]. The contents of TGF-b1 and TGF-b2 were evaluated after acidic activation and neutralization of the samples, to change their latent forms into immune-reactive forms. "
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