[Species of Ligularia in the northwestern China and their medicinal uses].
ABSTRACT To investigate the species of Ligularia distributed in the northwestern China and their medicinal uses in the local area.
Field investigation, specimen collection, taxonomic study and datum check were adopted.
There are 29 species and 1 varieties of Ligularia distributed in the northwestern China, and 18 species of them had been used as folk medicines with the function of resolving phlegm, relieving cough, clearing heat and toxins.
The northwestern China is abundant in medicinal resource of Ligularia.
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ABSTRACT: Gentiana dahurica Fisch is one of four important commercial Radix Gentianae stipulated by the Chinese Pharmacopoeia. We have established a rapid and effective regeneration system using zygotic embryo-derived callus of G. dahurica Fisch. Using this regeneration system, Agrobacterium tumefaciens-mediated transformation of G. dahurica Fisch has been developed. Zygotic embryo-derived callus was infected with an A. tumefaciens strain (GV3130) harboring a pBI121 vector that contained an nptII selective marker gene. In a total of 60 zygotic embryo-derived calli assayed, frequency of calli with nptII gene PCR-positive transgenic plantlets is 5%. Transient glucuronidase (GUS) expression was observed from these transgenic plantlets. Frequency of GUS-positive transgenic plantlets (4/78) was approximately consistent with that of PCR assay (3/60). Our data indicate that we have successfully established a stable G. dahurica Fisch transformation system by A. tumefaciens. In general, this transformation system we have established might be useful for modifying physiological, medicinal and horticultural traits. Keywords Gentiana dahurica Fisch-Somatic embryogenesis-Organogenesis-Glucuronidase (GUS)- npt II gene- Agrobacterium tumefaciensActa Physiologiae Plantarum 01/2010; 32(4):629-634. DOI:10.1007/s11738-009-0439-4 · 1.52 Impact Factor