Comparability of self-collected vaginal swabs and physician-collected cervical swabs for detection of human papillomavirus infections in Rakai, Uganda.
ABSTRACT The objective of this study was to compare human papillomavirus (HPV) DNA testing between self-administered vaginal swabs and physician-administered cervical swabs in women from rural Rakai District, Uganda.
Between 2002 and 2003, women from a population-based cohort participated in an HPV study. Women collected self-administered vaginal swabs and were also offered a pelvic examination, which included physician-collected cervical samples.
Hybrid-capture 2 was used to determine carcinogenic HPV status. Polymerase chain reaction was used to determine HPV genotypes. Unweighted kappa statistics were used to determine agreement.
Compliance with self-collected swabs was > or =86%; however, only 51% accepted a pelvic examination. Carcinogenic HPV prevalence was 19% in self-collected and 19% in physician-collected samples. Agreement among paired observations was 92% with a kappa of 0.75. Kappa between self- and physician-collected samples was similar in HIV strata (k = 0.71 and 0.75 for HIV-positive and HIV-negative, respectively).
In this community-based setting, detection of carcinogenic HPV was comparable among self- and physician-administered samples. Self-collection is a feasible and accurate means of obtaining HPV samples from women in resource-poor settings or persons reluctant to undergo a pelvic examination.
- SourceAvailable from: Spyros PournarasFuture Virology 04/2014; april 2014(9):385-395. · 1.00 Impact Factor
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ABSTRACT: To determine demographic and behavioural factors associated with HPV positivity in a community-based HPV self collection cervical cancer screening pilot project. HPV self collected samples were obtained from 199 women aged 30-69 years of age in the impoverished urban community of Kisenyi, Kampala during September to November 2011. Demographic and behavioural information was collected. Descriptive statistics and a logistic regression model were used to analyze factors associated with HPV positivity. There was overwhelming acceptance of HPV self collection in this community. High risk HPV prevalence was found to be 17.6%. Lower levels of formal education (AOR 0.40, 95%CI 0.08-2.03) were associated with higher prevalence of HPV as was use of oral contraception (AOR 2.01, (95%CI 0.83-4.90) and HIV status (AOR 0.43, 95%CI 0.14-1.37). Screening should be targeted and prioritized for women with lower levels of education, oral contraceptive use and HIV positive women as they have the highest HPV prevalence in this low-resource population.American journal of obstetrics and gynecology 08/2013; · 3.97 Impact Factor
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ABSTRACT: Self-sampling for Human Papillomavirus (HPV) testing may offer improved patient acceptability, decreased cost, and greater practicality than clinician collection of specimens. HPV testing among adolescents is necessary to conduct vaccine surveillance and may play a role in cervical cancer screening among some populations. A cross-sectional prevalence study was conducted to compare the results of self-collected and clinician-collected specimens for Human papillomavirus (HPV) testing among South African adolescent females. All participants provided self-sampled vaginal swabs and underwent clinician-collection of cervical swabs for HPV DNA analysis. The level of agreement between HPV DNA results from the two specimen collection methods was measured. The level of agreement between HPV DNA results from self-collected and clinician-collected specimens was high (κ=86.7; p<0.001). A high prevalence of HPV overall was found by both specimen collection methods (57%; 95% CI 0.37-0.75). Low-risk HPV (LR-HPV) types were found slightly more frequently in self-collected specimens. There is a high level of agreement between the HPV DNA results from self-collected and clinician-collected specimens. Self-collection of specimens for HPV testing is a viable alternative among adolescents.Journal of immunological techniques in infectious diseases. 09/2013; 2(3).