Article

Detection of cytomegalovirus in human placental cells by polymerase chain reaction.

Center for Medical Biology, Polish Academy of Sciences, Laboratory of Molecular Virology and Biological Chemistry, Lódź, Poland.
Apmis (Impact Factor: 2.07). 12/2006; 114(11):764-71. DOI: 10.1111/j.1600-0463.2006.apm_31.x
Source: PubMed

ABSTRACT Human cytomegalovirus (HCMV) is the most common cause of viral intrauterine infection. Progress in rapid, specific, and dependable detection of HCMV has recently been achieved by the use of DNA hybridization techniques and other molecular methods. We examined 21 placentas after delivery for the presence of HCMV DNA by polymerase chain reaction (PCR). To test the reliability of the PCR for the detection of HCMV DNA in clinical specimens, two simple PCR assays and a real-time quantitative PCR were used. PCR analysis of villous and decidual cells showed that HCMV DNA was present in 16 placentas (76.2%). Transmission of HCMV infection to chorionic villi was confirmed in 11 organs (52.4%), and congenital infections in newborns were detected in 9 cases (42.8%). These results suggest that HCMV genome detection in placentas at later gestational ages is common. Our results demonstrated that detection of HCMV DNA in placental tissues by DNA amplification provides a specific and sensitive method for diagnosis of intrauterine HCMV infection.

0 Bookmarks
 · 
61 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: TLRs play crucial role in non-specific immunity against various infections. The most common intrauterine infection is caused by HCMV, which result in perinatal morbidity and mortality of primary infected fetuses. The induction of immune response by TLRs was observed in HCMV infections in murine models and cell lines cultured in vitro. Studies reported immunological response in pregnant women primary infected with HCMV and TLR2 activity in collecting HCMV particles in placental ST in vivo and cultured ST and in stimulation of TNF-α expression and damage of villous trophoblast. Expression levels of TLRs associate with cell types, stage of pregnancy and response to microorganisms. In the article we showed the effect of HCMV infection on pregnancy development as well as TLR SNPs on the occurrence and course of infectious diseases, immune response and pregnancy diseases. We reported the impact of TLRs on the function of miRNAs and the altered expression levels of these molecules, observed in HCMV infections. We suggested that methylation status of TLR gene promoter regions as epigenetic modifications might be significant in immune response to HCMV infections. We concluded that it is important to study in details molecular mechanisms of TLRs function in immune response to HCMV infections in pregnancy. This article is protected by copyright. All rights reserved.
    Pathogens and disease. 08/2013;
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Objectives The association among specific single-nucleotide polymorphisms (SNPs) in TLR2 (Arg677Trp, Arg753Gln) and TLR4 (Asp299Gln) and human cytomegalovirus (CMV) infection was studied in infants and adults. Methods The TLR2 and TLR4 polymorphisms were genotyped in 151 patients with CMV infections and in 78 unrelated healthy individuals. Genotyping was performed by restriction fragment length polymorphism (RFLP) analysis of PCR-amplified fragments. The viral load was measured by quantitative real-time PCR. Results Almost all of the patients with CMV infections were wild-type homozygotes without TLR2 and TLR4 polymorphisms. No significant differences in TLR2 and TLR4 polymorphisms were observed between infants with or without CMV infection. Compared with adults with CMV infections, heterozygosity for the TLR2 Arg677Trp and TLR4 Asp299Gly SNPs was detected more frequently in healthy individuals (p < 0.05). Logistic regression analysis showed that the wild-type TLR2 genotype was associated with an increased risk of CMV infection and that heterozygosity for TLR2 and TLR4 SNPs diminished the risk of CMV infection in adult patients. An association between CMV load and the TLR4 SNP was found. Conclusion Our results suggest that the wild-type TLR2 genotype may be a risk factor for CMV replication in adult patients.
    International Journal of Infectious Diseases. 01/2014;
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The association among specific single-nucleotide polymorphisms (SNPs) in TLR2 (Arg677Trp, Arg753Gln) and TLR4 (Asp299Gln) and human cytomegalovirus (CMV) infection was studied in infants and adults. The TLR2 and TLR4 polymorphisms were genotyped in 151 patients with CMV infections and in 78 unrelated healthy individuals. Genotyping was performed by restriction fragment length polymorphism (RFLP) analysis of PCR-amplified fragments. The viral load was measured by quantitative real-time PCR. Almost all of the patients with CMV infections were wild-type homozygotes without TLR2 and TLR4 polymorphisms. No significant differences in TLR2 and TLR4 polymorphisms were observed between infants with or without CMV infection. Compared with adults with CMV infections, heterozygosity for the TLR2 Arg677Trp and TLR4 Asp299Gly SNPs was detected more frequently in healthy individuals (p<0.05). Logistic regression analysis showed that the wild-type TLR2 genotype was associated with an increased risk of CMV infection and that heterozygosity for TLR2 and TLR4 SNPs diminished the risk of CMV infection in adult patients. An association between CMV load and the TLR4 SNP was found. Our results suggest that the wild-type TLR2 genotype may be a risk factor for CMV replication in adult patients.
    International journal of infectious diseases: IJID: official publication of the International Society for Infectious Diseases 05/2014; · 2.17 Impact Factor

Full-text

View
4 Downloads
Available from
Jun 30, 2014