Determination of dopamine and its relativity of baicalin in rat nuclei after intravenous administration of flavonoids fromScutellariae radix

Laboratory of Pharmaceutical Sciences, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, People's Republic of China.
Biomedical Chromatography (Impact Factor: 1.72). 01/2007; 21(1):84-8. DOI: 10.1002/bmc.722
Source: PubMed


Baicalin is an active component of Scutellariae radix extracts. It can cross the blood-brain barrier and distribute in cerebral nuclei. However, its mechanism and the effects on the nuclei where it accumulates remain unclear. We used an HPLC-electrochemical detection method to determine the dopamine in cerebral nuclei after intravenous administration of flavonoids from Scutellariae radix and analyzed the relativity between baicalin and DA in cerebral nuclei. We found that the dopamine system is another target system of brain function which baicalin acts on.

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    • "Baicalin, the major component of the flavones, was confirmed with the neuroprotection of the damage by ischemia and reperfusion and the action on central nerve system [7] [8] [9] [10]. "
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    ABSTRACT: This work is to study the baicalin and its three analogs, baicalin, wogonoside, and wogonin, on the protective effect of neuron from oxygen-glucose deprivation (OGD) and toll-like receptor 2 (TLR2) expression in OGD damage. The results showed that baicalin and its three analogs did protect neurons from OGD damage and downregulated protein level of TLR2. D-Glucopyranosiduronic acid on site 7 in the structure played a core of cytotoxicity of these flavonoid analogs. The methoxyl group on carbon 8 of the structure had the relation with TLR2 protein expression, as well as the anti-inflammation. In addition, we detected caspase3 and antioxidation capability, to investigate the effect of four analogs on cell apoptosis and total antioxidation competence in OGD model.
    BioMed Research International 03/2012; 2012:267890. DOI:10.1155/2012/267890 · 2.71 Impact Factor
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    • "Previous studies have suggested that baicalin can rapidly pass the blood brain barrier with drug concentrations higher in brain nuclei after intravenous (i.v.) administration of total flavonoids of SR [6-8] . Also, a significant correlation was found between the positive increase of the baicalin and dopamine concentrations [9] , suggesting baicalin may directly affect the brain functions . The current experiments test the anti-depressive effect of baicalin administered by the intraperitoneal (i.p.) route to mice, using the classical tail suspension Received: 2009-06-11; revised: 2010-06-02 * Supported by the National Natural Science Foundation of China (Nos. "
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    ABSTRACT: A series of animal models are used to investigate the anti-depression mechanism of flavonoids in scutellariae radix (SR) in vivo. Depression-like behavior in mice was studied after intraperitoneal administration of SR. The results showed that SR administered to mice by the intraperitoneal route obviously shortened the duration in the tail suspension test and the forced swimming test, aggravated the symptoms of eyelid ptosis, akinesia, and mortality caused by reserpine, prolonged climbing times, affected the conditioned place preference, and increased sugar consumption in mice. However the SR did not affect the head twitches induced by 5-HTP, locomotor activity in mice, the toxicity of yohimbine, and the body temperature decrease caused by high dosage of apomorphine. The tests show that SR has some anti-depression effect related to the dopamine system. Furthermore another anti-depression mechanism was possible that could affect the mechanism of brain reward, bring positive reinforcement, and increase the sensitivity to euphoria in mice.
    Tsinghua Science & Technology 08/2010; 15(4-15):460-466. DOI:10.1016/S1007-0214(10)70088-2
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    ABSTRACT: The amphetamine (AMPH)-induced alteration in rat brain dopamine levels modified by N-acetylcysteine (NAC) administration has been examined using isocratic ion-pair reversed-phase high-performance liquid chromatography with electrochemical detection. The aim of the development of a novel validated evaluation scheme implying a double AMPH challenge was to enhance the efficiency of AMPH-triggered dopamine release measurements in rat brain striatal slices by improving the reproducibility of the results. The proposed experimental protocol was tested in vivo and proved to be capable of fast and reliable drug screening for tracing the effect of NAC as a model compound in AMPH-mediated dopaminergic response. The subcellular localization of the dopamine mobilizing effect of NAC has been established indirectly by the use of an irreversible dopamine vesicular depletor, reserpine. The antioxidant NAC at 10 mM plays an important role in the complete suppression of acute AMPH-elicited dopamine release. The possible role of this quenching effect is discussed.
    Biomedical Chromatography 06/2009; 23(6):658-64. DOI:10.1002/bmc.1171 · 1.72 Impact Factor
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