Article

Supplemental testing is still required in australia for samples positive for Neisseria gonorrhoeae by nucleic acid detection tests.

Journal of Clinical Microbiology (impact factor: 4.15). 12/2006; 44(11):4292-4; author reply 4293-4. DOI:10.1128/JCM.01577-06 pp.4292-4; author reply 4293-4
Source: PubMed
0 0
 · 
0 Bookmarks
 · 
13 Views
  • Source
    Article: DNA sequencing validation of Chlamydia trachomatis and Neisseria gonorrhoeae nucleic acid tests.
    Sin Hang Lee, Veronica S Vigliotti, Suri Pappu
    [show abstract] [hide abstract]
    ABSTRACT: DNA sequencing was used to confirm Chlamydia trachomatis and Neisseria gonorrhoeae nucleic acids in endocervical swab samples. DNA in residues of the samples with positive results by 2 commercial kits was subjected to nested polymerase chain reaction (PCR) amplification. The nested PCR amplicons were used as templates for direct automated DNA sequencing. A 40-base signature sequence was sufficient to achieve unequivocal validation of C trachomatis cryptic plasmid and gonococcal opa gene DNA. DNA with a signature sequence specific for C trachomatis was identified in all 14 samples and for N gonorrhoeae in all 13 samples with positive results by the commercial kits for these 2 microbes. In a low-prevalence population, PCR retesting of 289 samples with initial negative results by a non-nucleic acid amplification assay revealed 3 samples positive for C trachomatis and 2 samples positive for N gonorrhoeae that were missed by the commercial kit. DNA sequencing is a useful tool in validating molecular diagnostics.
    American Journal of Clinical Pathology 07/2008; 129(6):852-9. · 2.60 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.