Article

Cysteinyl leukotriene receptors CysLT1 and CysLT2 are upregulated in acute neuronal injury after focal cerebral ischemia in mice.

Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou 310058, China.
Acta Pharmacologica Sinica (impact factor: 1.95). 01/2007; 27(12):1553-60. DOI:10.1111/j.1745-7254.2006.00458.x pp.1553-60
Source: PubMed

ABSTRACT To determine whether cysteinyl leukotriene receptors (CysLT1 and CysLT2) are upregulated in acute neuronal injury after focal cerebral ischemia in mice, and to confirm CysLT1 receptor localization.
After permanent focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO), neurological deficits and neuron loss were determined at various time points within 48 h. The mRNA expressions of CysLT1 and CysLT2 receptors in the brain were analyzed by RT-PCR. CysLT1 receptor localization was detected by double immunofluorescence.
Neurological deficits and neuron loss were found 6, 24 and 48 h after MCAO. The mRNA expressions of both CysLT1 and CysLT2 receptors were upregulated in the ischemic hemisphere 1, 24, and 48 h after MCAO with peaks at 24 h. The CysLT1 receptor was selectively localized in neurons 24 h after MCAO.
CysLT1 and CysLT2 receptors are upregulated in acute neuronal injury after focal cerebral ischemia, and the CysLT1 receptor is localized in neurons after ischemia.

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Keywords

acute neuronal injury
 
CysLT1
 
CysLT1 receptor
 
CysLT1 receptor localization
 
CysLT2 receptors
 
cysteinyl leukotriene receptors
 
double immunofluorescence
 
focal cerebral ischemia
 
ischemia
 
ischemic hemisphere 1
 
mice
 
middle cerebral artery occlusion
 
mRNA expressions
 
neurological deficits
 
neurons
 
neurons 24 h
 
permanent focal cerebral ischemia
 
various time points
 

Yan-Jun Zhang