Article

Mycobacterium kumamotonense Sp. Nov. recovered from clinical specimen and the first isolation report of Mycobacterium arupense in Japan: Novel slowly growing, nonchromogenic clinical isolates related to Mycobacterium terrae complex.

Gifu University Graduate School of Medicine, Gifu, Japan.
Microbiology and Immunology (impact factor: 1.3). 02/2006; 50(11):889-97. pp.889-97
Source: PubMed

ABSTRACT Three mycobacterium strains isolated from clinical specimens in Japan were provisionally assigned to the genus Mycobacterium based on their phenotypical characteristics. These isolates were further investigated to determine their specific taxonomic statuses. Mycolic acid analysis and 16S rRNA gene, rpoB, and hsp65 sequence data for the isolates showed that they are most similar to M. terrae complex. DNA-DNA hybridization studies indicated that the three strains were of two species and were distinguishable from M. terrae, M. nonchromogenicum, and M. hiberniae. Therefore, these strains represent two novel species within the genus Mycobacterium. However, one potential new species should have been considered as M. arupense with the 16S rRNA gene and hsp65 sequences similarities of 99.8% and 100% respectively; it was isolated from human specimens in the United States and was proposed in June 2006 as a new species. This report describes the first isolation of M. arupense in Japan, suggesting that the organism is clinically relevant. In addition, we propose the novel species designation Mycobacterium kumamotonense sp. nov. The type strain is CST 7247(T) (=GTC 2729(T), =JCM 13453(T), =CCUG 51961(T)).

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    Article: Isolation of non-tuberculous mycobacteria from pastoral ecosystems of Uganda: public health significance.
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    BMC Public Health 05/2011; 11:320. · 2.00 Impact Factor
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Keywords

clinical specimens
 
first isolation
 
genus Mycobacterium
 
hsp65 sequence data
 
human specimens
 
M. arupense
 
M. hiberniae
 
M. nonchromogenicum
 
M. terrae
 
M. terrae complex
 
mycobacterium strains
 
Mycolic acid analysis
 
new species
 
novel species
 
novel species designation Mycobacterium kumamotonense sp
 
potential new species
 
rpoB
 
specific taxonomic statuses
 
three strains
 
United States