Progress in Raman spectroscopy in the fields of tissue engineering, diagnostics and toxicological testing.

Department of Materials, Imperial College London, Prince Consort Road, London, SW7 2AZ, United Kingdom.
Journal of Materials Science Materials in Medicine (Impact Factor: 2.38). 12/2006; 17(11):1019-23. DOI: 10.1007/s10856-006-0438-6
Source: PubMed

ABSTRACT This review summarises progress in Raman spectroscopy and its application in diagnostics, toxicological testing and tissue engineering. Applications of Raman spectroscopy in cell biology are in the early stages of development, however, recent publications have demonstrated its utilisation as a diagnostic and development tool with the key advantage that investigations of living cells can be performed non-invasively.Some of the research highlighted here demonstrates the ability of Raman spectroscopy to accurately characterise cancer cells and distinguish between similar cell types. Many groups have used Raman spectroscopy to study tissues, but recently increased effort has gone into single cell analysis of cell lines; the advantages being that cell lines offer ease of handling and increased reproducibility over tissue studies and primary cells. The main goals of bio-Raman spectroscopy at this stage are twofold. Firstly, the aim is to further develop the diagnostic ability of Raman spectroscopy so it can be implemented in a clinical environment, producing accurate and rapid diagnoses. Secondly, the aim is to optimise the technique as a research tool for the non-invasive real time investigation of cell/material interactions in the fields of tissue engineering and toxicology testing.

  • [Show abstract] [Hide abstract]
    ABSTRACT: In this study, confocal Raman spectroscopy, atomic force microscope (AFM) and multiplex ELISA were applied to analyze the biophysical responses (biomechanics and biospectroscopy) of normal human primary small airway epithelial cells (SAECs) and human lung carcinoma epithelial A549 cells to in vitro short term DEP exposure (up to 2 h). Raman spectra revealed the specific cellular biomolecular changes in cells induced by DEP compared to unexposed control cells. Principal component analysis was successfully applied to analyze spectral differences between control and treated groups from multiple individual cells, and indicated that cell nuclei are more sensitive than other cell locations. AFM measurements indicated that 2 h of DEP exposure induced a significant decrease in cell elasticity and a dramatic change in membrane surface adhesion force. Cytokine and chemokine production measured by multiplex ELISA demonstrated DEP-induced inflammatory responses in both cell types.
    Toxicology Letters 12/2012; 215(3):181–192. · 3.36 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Differences between Raman spectra of normal, malignant and benign tissues have been recorded and analyzed as a method for the early detection of cancer. To the best of our knowledge, this is one of the most statistically reliable research (67 patients) on Raman spectroscopy-based diagnosis of breast cancers among the world women population. The paper demonstrates that Raman spectroscopy is a promising new tool for real-time diagnosis of tissue abnormalities.
    Spectroscopy 01/2008; 22. · 0.83 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The activation of lymphocytes occurs when they are exposed to viruses or other foreign antigens. The aim of this work was to verify if Raman spectroscopy can be used to screen the activation of lymphocytes during viral infection. There are distinct peaks that reveal differences between activated and intact cells. The most important marker of the lymphocyte activation process is the prominent 521 cm(-1) disulfide band which marks the immunoglobulin formation. The up shift of the S-S mode from the broad band centered at 510 cm(-1) of human normal immunoglobulin to a single band at 521 cm(-1) of human B cells indicates a selection of the optimal geometry of the disulphide bridges to bind to a foreign antigen. Polarization data is used to detect the structural alteration between domain fragments. Differences in other band intensities may be due to different protein compositions in both the investigated forms. B cell activation causes the change of the intracellular cytoplasm composition due to the secretion of immunoglobulins during the fighting of the infection.
    The Analyst 10/2013; · 3.91 Impact Factor