Survey of the year 2005 commercial optical biosensor literature.

Center for Biomolecular Interaction Analysis, University of Utah, Salt Lake City, UT 84132, USA.
Journal of Molecular Recognition (Impact Factor: 2.34). 11/2006; 19(6):478-534. DOI: 10.1002/jmr.808
Source: PubMed

ABSTRACT We identified 1113 articles (103 reviews, 1010 primary research articles) published in 2005 that describe experiments performed using commercially available optical biosensors. While this number of publications is impressive, we find that the quality of the biosensor work in these articles is often pretty poor. It is a little disappointing that there appears to be only a small set of researchers who know how to properly perform, analyze, and present biosensor data. To help focus the field, we spotlight work published by 10 research groups that exemplify the quality of data one should expect to see from a biosensor experiment. Also, in an effort to raise awareness of the common problems in the biosensor field, we provide side-by-side examples of good and bad data sets from the 2005 literature.

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    ABSTRACT: Microbubble science is expanding beyond ultrasound imaging applications to biological targeting and drug/gene delivery. The characteristics of molecular targeting should be tested by a measurement system that can assess targeting efficacy and strength. Atomic force microscopy (AFM) is capable of piconewton force resolution, and is reported to measure the strength of single hydrogen bonds. An in-house targeted microbubble modified using the biotin-avidin chemistry and the CD31 antibody was used to probe cultures of Sk-Hep1 hepatic endothelial cells. We report that the targeted microbubbles provide a single distribution of adhesion forces with a median of 93pN. This interaction is assigned to the CD31 antibody-antigen unbinding event. Information on the distances between the interaction forces was obtained and could be important for future microbubble fabrication. In conclusion, the capability of single microbubbles to target cell lines was shown to be feasible with AFM.
    Colloids and surfaces B: Biointerfaces 10/2010; 80(1):12-7. DOI:10.1016/j.colsurfb.2010.05.022 · 4.29 Impact Factor
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    ABSTRACT: In the past, surface plasmons have been used to improve the surface sensitivity of several spectroscopic measurements. Surface plasmon resonance reflectivity measurements can be used to detect biomolecules and their interactions by changes in the local index of refraction upon adsorption of the target molecule to the metal surface. Using this principle several SPR-based biosensors have been generated in the past with wide range of applications in the field of biology, biomedicine and biochemistry. Among several techniques available for biomolecular interactions, SPR-based Biacore is one of the widely used real-time monitoring systems and proved to be a convenient system for wide range of molecular sizes from small ligands to whole cells. Biacore system can be employed for biomolecular interactions, selection procedures, epitope mapping, molecular discriminations, kinetic analyses, screening processes and other applications. There were several successes reported using the versatility of Biacore system. In this report, several biosensing applications of this system were overviewed.
    Sensors and Actuators B Chemical 10/2010; DOI:10.1016/j.snb.2010.08.014 · 3.84 Impact Factor
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    ABSTRACT: A transmission-based surface plasmon resonance (SPR) sensor for label-free detection of protein-carbohydrate and protein-protein binding proximate to a perforated gold surface is demonstrated. An SPR instrument makes real-time measurements of the resonant wavelength and/or the resonant angle of incidence of transmitted light; both are influenced by the presence of proteins at the gold surface-liquid interface. Ethylene glycol solutions with known refractive indexes were used to calibrate the instrument. A paired polarization-sensitive detector achieved an overall detection resolution of ~ 6.6 times 10<sup>-5</sup> refractive index units (RIU). Proof of principle experiments was performed with concanavalin A (Con A) binding to gold-adsorbed ovomucoid and anti-bovine serum albumin (BSA) binding to gold-adsorbed BSA.
    IEEE Sensors Journal 01/2009; DOI:10.1109/JSEN.2008.2007663 · 1.85 Impact Factor


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