Functional and quantitative proteomics using SILAC.
ABSTRACT Researchers in many biological areas now routinely characterize proteins by mass spectrometry. Among the many formats for quantitative proteomics, stable-isotope labelling by amino acids in cell culture (SILAC) has emerged as a simple and powerful one. SILAC removes false positives in protein-interaction studies, reveals large-scale kinetics of proteomes and - as a quantitative phosphoproteomics technology - directly uncovers important points in the signalling pathways that control cellular decisions.
- SourceAvailable from: Mathias Uhlen[Show abstract] [Hide abstract]
ABSTRACT: Resolving the molecular details of proteome variation in the different tissues and organs of the human body will greatly increase our knowledge of human biology and disease. Here, we present a map of the human tissue proteome based on an integrated omics approach that involves quantitative transcriptomics at the tissue and organ level, combined with tissue microarray-based immunohistochemistry, to achieve spatial localization of proteins down to the single-cell level. Our tissue-based analysis detected more than 90% of the putative protein-coding genes. We used this approach to explore the human secretome, the membrane proteome, the druggable proteome, the cancer proteome, and the metabolic functions in 32 different tissues and organs. All the data are integrated in an interactive Web-based database that allows exploration of individual proteins, as well as navigation of global expression patterns, in all major tissues and organs in the human body. Copyright © 2015, American Association for the Advancement of Science.Science (New York, N.Y.). 01/2015; 347(6220):1260419.
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ABSTRACT: Background Ubiquitination is a highly dynamic and reversible process with a central role in cell homeostasis. Deregulation of several deubiquitinating enzymes has been linked to tumor development but their specific role in prostate cancer progression remains unexplored.MethodsRNAi screening was used to investigate the role of the ovarian tumor proteases (OTU) family of deubiquitinating enzymes on the proliferation and invasion capacity of prostate cancer cells. RhoA activity was measured in relation with OTUB1 effects on prostate cancer cell invasion. Tumor xenograft mouse model with stable OTUB1 knockdown was used to investigate OTUB1 influence in tumor growth.ResultsOur RNAi screening identified OTUB1 as an important regulator of prostate cancer cell invasion through the modulation of RhoA activation. The effect of OTUB1 on RhoA activation is important for androgen-induced repression of p53 expression in prostate cancer cells. In localized prostate cancer tumors OTUB1 was found overexpressed as compared to normal prostatic epithelial cells. Prostate cancer xenografts expressing reduced levels of OTUB1 exhibit reduced tumor growth and reduced metastatic dissemination in vivo. ConclusionsOTUB1 mediates prostate cancer cell invasion through RhoA activation and promotes tumorigenesis in vivo. Our results suggest that drugs targeting the catalytic activity of OTUB1 could potentially be used as therapeutics for metastatic prostate cancer.Molecular cancer. 01/2015; 14(1):8.
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ABSTRACT: Plumbagin (PLB) has exhibited a potent anticancer effect in preclinical studies, but the molecular interactome remains elusive. This study aimed to compare the quantitative proteomic responses to PLB treatment in human prostate cancer PC-3 and DU145 cells using the approach of stable-isotope labeling by amino acids in cell culture (SILAC). The data were finally validated using Western blot assay. First, the bioinformatic analysis predicted that PLB could interact with 78 proteins that were involved in cell proliferation and apoptosis, immunity, and signal transduction. Our quantitative proteomic study using SILAC revealed that there were at least 1,225 and 267 proteins interacting with PLB and there were 341 and 107 signaling pathways and cellular functions potentially regulated by PLB in PC-3 and DU145 cells, respectively. These proteins and pathways played a critical role in the regulation of cell cycle, apoptosis, autophagy, epithelial to mesenchymal transition (EMT), and reactive oxygen species generation. The proteomic study showed substantial differences in response to PLB treatment between PC-3 and DU145 cells. PLB treatment significantly modulated the expression of critical proteins that regulate cell cycle, apoptosis, and EMT signaling pathways in PC-3 cells but not in DU145 cells. Consistently, our Western blotting analysis validated the bioinformatic and proteomic data and confirmed the modulating effects of PLB on important proteins that regulated cell cycle, apoptosis, autophagy, and EMT in PC-3 and DU145 cells. The data from the Western blot assay could not display significant differences between PC-3 and DU145 cells. These findings indicate that PLB elicits different proteomic responses in PC-3 and DU145 cells involving proteins and pathways that regulate cell cycle, apoptosis, autophagy, reactive oxygen species production, and antioxidation/oxidation homeostasis. This is the first systematic study with integrated computational, proteomic, and functional analyses revealing the networks of signaling pathways and differential proteomic responses to PLB treatment in prostate cancer cells. Quantitative proteomic analysis using SILAC represents an efficient and highly sensitive approach to identify the target networks of anticancer drugs like PLB, and the data may be used to discriminate the molecular and clinical subtypes, and to identify new therapeutic targets and biomarkers, for prostate cancer. Further studies are warranted to explore the potential of quantitative proteomic analysis in the identification of new targets and biomarkers for prostate cancer.Drug Design, Development and Therapy 01/2015; 9:349-417. · 3.03 Impact Factor
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Competing interests statement
The authors declare no competing financial interests.
Daniel Axelrod’s homepage: http://www.physics.lsa.umich.
Geneva M. Omann’s homepage: http://www.biochem.med.
Access to this links box is available online.
Functional and quantitative
proteomics using SILAC
Abstract | Researchers in many biological areas now routinely characterize proteins
by mass spectrometry. Among the many formats for quantitative proteomics,
stable-isotope labelling by amino acids in cell culture (SILAC) has emerged as a
simple and powerful one. SILAC removes false positives in protein-interaction
studies, reveals large-scale kinetics of proteomes and — as a quantitative
phosphoproteomics technology — directly uncovers important points in the
signalling pathways that control cellular decisions.
Proteins are in direct control of almost
all cellular processes, and post-genomic
biology will not reach its potential until we
have tools to study proteins on a large scale.
Sadly, proteomics has lagged far behind
DNA-based technologies, mainly because
there are no protein-analysis methods
similar to oligonucleotide hybridization,
amplification and sequencing. This lag
is diminishing rapidly, however, due to
increasingly powerful mass spectrometry
(MS)-based technologies1. Mass spectro-
meters can sequence thousands of peptides
from complex mixtures in an automated
manner2. The importance of quantitation in
proteomics has recently become appreciated,
and technologies such as isotope-encoded
affinity tags (ICAT)3 have generated wide-
spread interest. Much of this interest is still
focused on the determination of the relative
levels of expression of, ideally, all proteins
between two cell or tissue states (expression
proteomics). Expression proteomics has also
been the elusive goal of the two-dimensional
gel electrophoresis community and has
essentially the same principle at the protein
level as microarrays have at the mRNA level
However, proteomics — unlike transcrip-
tomics — is not limited to measuring
whole cell or tissue expression levels. Here,
I argue that the most important contribu-
tions of quantitative proteomics to biological
understanding will come from its unique
capability to determine changes in function-
ally relevant ‘sub-proteomes’. Although many
of the strategies described here can be, and
sometimes have been, implemented with
other quantitative-proteomics techniques,
I also argue that stable-isotope labelling
by amino acids in cell culture (SILAC) com-
bined with sophisticated mass-spectrometric
and bioinformatic technology is particularly
well suited to reinvent or enhance bio-
chemistry-based approaches. This argument
will be made, in part, by describing success-
ful applications of SILAC to a wide range of
952 | DECEMBER 2006 | VOLUME 7
© 2006 Nature Publishing Group
First, I explain the principles of SILAC
and its advantages and limitations compared
with other quantitative-proteomics strate-
gies. SILAC has been used successfully for
the determination of cholesterol-dependent
lipid rafts and the elucidation of protein
interactions, side-stepping the longstanding
trade-off between assuring specificity of
the measured interaction and preservation
of weak interactions. I then provide some
examples of the quantitation of changes in
the phosphoproteome from the yeast phero-
mone pathway to stem-cell differentiation.
Furthermore, SILAC has become a prime
technology to add a time dimension to
proteo mics. Last, I describe the emerging use
of SILAC to directly study protein synthesis
and degradation. As we reach the conclusions
and perspectives, I hope that the reader will
appreciate how SILAC-based proteo mics
fundamentally transforms the questions
we can ask using biochemical approaches.
Indeed, the emergence of the SILAC strategy
and the extraordinary progress in mass-
spectrometric technology in just the past few
years now offers exciting new strategies for
advances in virtually all areas of biology.
Principles of SILAC
SILAC was first described in the literature
only four years ago4,5, later than many other
schemes for quantitative proteomics (REF. 6;
see also reviews7–11 for different aspects of
the field). It is conceptually and experimen-
tally straightforward: it involves growing two
populations of cells, one in a medium that
contains a ‘light’ (normal) amino acid and
the other in a medium that contains a ‘heavy’
amino acid. The heavy amino acid can con-
tain 2H instead of H, 13C instead of 12C,
or 15N instead of 14N. Incorporation of the
heavy amino acid into a peptide leads to a
known mass shift compared with the peptide
that contains the light version of the amino
acid (for example, 6 Da in the case of
13C6-Arg), but to no other chemical changes.
In some respects, SILAC is similar to
other metabolic labelling techniques that
have been used for decades in biological
research (for advantages and limitations of
SILAC compared with other quantitative-
proteomics technologies, see BOX 1).
For example, in pulse-chase experiments,
a radioactive form of an amino acid is added
to a medium with cells for a certain length
of time and the radioactivity that is incor-
porated into the newly synthesized proteins
is measured. However, in contrast to pulse-
chase experiments, the isotopes of SILAC
amino acids are stable — no radioactivity
is involved. Furthermore, the objective in
SILAC is to distinguish two proteomes by
the molecular weight of the light or heavy
amino acid that is used during the growth
of the two cell populations. This requires
complete labelling — the SILAC amino acid
in all proteins should be replaced — and this
is achieved after five cell doublings, even for
proteins with no significant turnover.
To ensure that cells only incorporate the
added, labelled amino acid into their pro-
teome, essential amino acids are chosen and
cells are grown with dialyzed serum, when
appropriate. Some cell types require low
molecular mass growth factors, which are
not present in dialyzed serum. In these cases,
exogenous growth factors or serum dialyzed
with a low molecular mass cut-off can be
used. Alternatively, the use of a small amount
of undialyzed serum — not sufficient to
significantly influence quantitation — has
proven useful12. Early concerns that the
isotope labels would be scrambled through
metabolic cycles that involve different amino
acids have proven unfounded. An exception
is Arg, which is converted to Pro by some
cell types when present at high levels in the
media13. Peptides that contain both Arg and
Pro can be quantified separately, or Arg in
the media can be titrated down so that Pro
conversion becomes negligible and cells can
still grow normally.
In a straightforward expression-
proteo mics experiment, one cell popula-
tion is labelled with a light amino acid
(population A) and another cell population
is labelled with a heavy amino acid (popula-
tion B). Then the cells are mixed and their
prote o mes are extracted and measured by
MS. Each peptide appears as a pair in the
mass spectra — the peptide with lower mass
contains the light amino acid and originates
from population A, and the peptide with
higher mass contains the heavy amino acid
and originates from population B. If the
SILAC peptide pair appears in a one-to-one
ratio then there is no difference in the abun-
dance of this protein between the proteomes.
A higher peak intensity from the peptide that
contains the heavy amino acid indicates
that the protein was more abundant in
population B. Because the light and heavy
amino acids are chemically identical, except
for their mass difference, the ratio of peak
intensities in the mass spectrometer directly
yields the ratio of the proteins in population
A versus population B.
Accuracy of quantitation depends on the
abundance and signal-to-noise ratio of the
peptide pair, and can be as good as a few
percent14. Usually, ratios of 1.3–2.0-fold have
been used as cut-offs for both statistical and
biological significance. For example, Everly
et al. used SILAC to compare microsomal
fractions of a more metastatic versus a
less metastatic prostate-cancer cell line15,16.
A total of 1,395 proteins were quantified, and
20% of these were differentially expressed
by more than 3-fold between the 2 cell lines.
Box 1 | Advantages and limitations of SILAC
Mass spectrometry is not inherently quantitative, as different molecules have different mass
spectrometric responses. Stable-isotope analogues of the molecule to be quantified have
therefore been used for many years for accurate quantitation in small-molecule mass spectrometry.
To differentially quantify two proteomes, a stable isotope can be introduced in various ways, most
commonly by chemical modification or by metabolic labelling6. Chemical labelling can be done on
any proteome, including body fluids and biopsis material, whereas metabolic strategies require
living cells. Chemical strategies involve a derivatization step that might not be complete, that
might introduce side products, and that might limit the sensitivity of the analysis.
Another important difference between metabolic and chemical labelling is the labelling stage. In
chemical labelling, the two proteomes to be compared have to be purified and fractionated
separately and in precisely the same way to allow relative quantitation of the same fractions. By
contrast, metabolic labelling allows mixing of labelled and unlabelled cells and therefore
subsequent fractionation and purification steps will not introduce any errors in quantitation.
Metabolic labelling for quantitation was first introduced to proteomics by the Langen group in
1998 by feeding microbes a 15N-substituted food source (described in REF. 55) and it was also used
shortly afterwards for quantitation by the Chait group56. The stable-isotope labelling by amino
acids in cell culture (SILAC) strategy has a number of advantages with respect to 15N labelling. For
instance, in SILAC, the expected mass differences are known before peptide identification,
simplifying quantitation. Furthermore, mammalian cell lines are easily labelled by providing the
SILAC amino acid instead of having to eliminate any unlabelled nitrogen source from the media
and — because only one or two amino acids in a peptide are substituted and the degree of
labelling is very high — quantitation is straightforward. SILAC does require adapting one’s cell line
to dialyzed serum, and further steps might have to be taken if cells don’t adapt well. However, this
step can be checked without labelled amino acids. The SILAC approach is not necessarily
expensive, especially if amino acids are bought directly and in gram amounts from a supplier.
NATURE REVIEWS | MOLECULAR CELL BIOLOGY
VOLUME 7 | DECEMBER 2006 | 953
© 2006 Nature Publishing Group
(counts per s)
(counts per s)
(counts per s)
(counts per s)
(counts per s × 10–2)
Elution time (min)
(counts per s × 10–2)
Elution time (min)
Ratio > 10Ratio = 1.73
Grown with normal Leu
filipin or nystatin
Lyse cells in 1% Triton X-100
Density gradient centrifugation to isolate
low-density fraction and enrich ra?s
Grown with LeuD3
Gene-ontology analysis revealed that 10%
of the most highly downregulated proteins
in the more metastatic cell line are involved
in cell adhesion. Similarly, Gronborg et al.
used SILAC to compare secreted proteins
from pancreatic-cancer-derived cells with
non-neoplastic pancreatic cells and found a
number of differentially expressed proteins17.
In contrast to expression proteomics,
which aims to quantify the complete prote-
ome, in a ‘functional’ SILAC experiment
one cell population functions as a control,
whereas the other one is perturbed in
some way and a sub-proteome of interest is
isolated (BOX 2). The SILAC approach itself
is straightforward, but identifying the most
elegant perturbation and control might
require considerable ingenuity.
Eliminating false-positive interactions
One of the most spectacular applications
of proteomics has been the elucidation of
protein-interaction networks. Specifically,
two large-scale studies in yeast used
immuno precipitation followed by MS,
and these were influential in promoting
the view that most proteins exist as parts
of multiprotein complexes rather than as
solitary factors18,19. Unfortunately, these
studies are extremely difficult to control for
false-positive inter actions20,21. The problem
is that MS-based proteomics is now so
sensitive that any pull-down assay, regard-
less of bait, will result in a large number
of identified background proteins. More
specific and stringent purification, such as
Box 2 | Principle of a functional SILAC experiment, exemplified by the lipid-raft proteome
Lipid rafts are areas of the plasma membrane
with specific lipid and protein composition
that are thought to organize signalling
events57; however, the concept of lipid rafts is
controversial58, partly because rafts cannot
be directly visualized or purified completely.
To investigate the existence of lipid rafts
(panel a), we used a deuterated-Leu-labelled
(LeuD3) cell population as a control and a
normal Leu-labelled population that was
treated with cholesterol-disrupting agents59.
We then combined both cell populations and
enriched for detergent-resistant lipid rafts in
the standard way. Lipid rafts are destroyed
in the treated, but not the untreated, stable-
isotope labelling by amino acids in cell
culture (SILAC)-labelled cell populations, and
cells are combined.
The actual mass spectrometry (MS) data for
peptides from two different proteins is
shown in panel b. The SILAC peptide pair at
mass-to-charge ratio (m/z) 709.89 and
712.903 had higher intensity in the heavy-
amino-acid form. Peptide sequencing
(MS/MS; middle) identified the peptide as
originating from flotillin-1, and the extracted
ion current (XIC; peptide ion signal as a
function of elution time; bottom) indicates
the total signal per peptide (see REF. 60 for
an introduction to peptide sequencing).
Clearly the integrated signal of the
light form is much less than that of the heavy
form, indicating that the cholesterol-
disrupting drug removed flotillin-1 from the
preparation. By contrast, a peptide from
β-tubulin (SILAC peaks at m/z 520.302 and
521.815) is present even when rafts are
destroyed (ratio 1.73, which is not
statistically significant in this experiment)
and this protein is therefore not a genuine
member of lipid rafts.
MS analysis identified 703 proteins, but
only 241 of these had statistically significant
fold changes, indicating that they were
depleted by removal of cholesterol.
By definition, these 241 proteins are
members of cholesterol-dependent lipid
rafts. Figure redrawn with permission from
REF. 59 © (2003) National Academy of
954 | DECEMBER 2006 | VOLUME 7
© 2006 Nature Publishing Group
Logs of SILAC ratios
with the tandem affinity purification (TAP)
tag22, is an improvement but not a panacea,
as low-affinity interactions tend to be lost.
Both the Aebersold group, using ICAT,
and my group, using SILAC, showed that
quantitative proteomics can completely
overcome the problem of nonspecifically
interacting proteins5,23,24. The basic strategy is
explained in FIG. 1 using a phosphodependent
protein–protein interaction that involves
a bacterial protein, which is injected into a
human host cell in the course of bacterial
infection. Following the synthesis of both
non-phosphorylated and phosphorylated
forms of the peptide that contains the phos-
phorylation site of the bacterial protein, the
non-phosphorylated and phosphorylated
peptides were incubated with light- and
heavy-amino-acid-containing cell lysates,
respectively. After gentle washing to preserve
weak interactions, bound proteins were eluted
and mixed. The vast majority of the proteins
that were identified in the mixed eluates were
present in a one-to-one ratio, which indicates
that they were binding nonspecifically to the
beads or to the peptide. Proteins that bind
specifically as a result of phosphorylation are
easily distinguishable because of their statisti-
cally significant ratios. As a further control,
the phosphorylated peptide can be incubated
with the heavy-amino-acid-containing lysate
and the non-phosphorylated peptide with the
light-amino-acid-containing lysate. In this
‘cross-over’ experiment, all SILAC-peptide
ratios should be inverted.
We scaled up this technology to identify
the interactome of all potentially Tyr-
phosphorylated residues of the ERBB-
receptor family. Our findings recapitulated
many of the interactions that were identified
over the past 15 years. Furthermore, some
new interactions, which implicate epidermal
growth factor receptor (EGFR) and ERBB4
in more diverse signalling roles than ERBB2
and ERBB3, were also identified25. All
interactors with a statistically significant
SILAC ratio had phosphotyrosine-binding
domains. This finding, compared with data
already available in the literature, indicated
that the screen did not result in any
Identifying binding partners of PP1. The
principle outlined above can be used with
any protein interactions as long as bait and
control are available and specific interacting
proteins are expressed in sufficient amounts
for detection by MS. Trinkle-Mulcahy et al.
tagged isoforms of protein phosphatase-1
(PP1) with green fluorescent protein (GFP)
and used SILAC to identify and distinguish
their binding partners26. Three cell states
were labelled for pull-down with control–
GFP, PP1α–GFP and PP1γ–GFP. Out of the
hundreds of the proteins that were identified
with anti-GFP antibody immunoprecipita-
tion, the SILAC ratios showed that some
proteins bind to all PP1 isoforms, whereas
others bind specifically to PP1α or PP1γ.
This study also identified a new protein,
Repo-Man, which binds to PP1γ, targets PP1
to chromatin and seems to have important
functions in the cell cycle27.
SILAC is currently used in a similar man-
ner to probe protein interactions of immo-
bilized drugs. In our laboratory we have also
used SILAC to identify transcription factors
and cofactors by incubating double-stranded
synthetic DNA with nuclear extract. Any
protein that preferentially binds to the wild-
type DNA compared to a point-mutated
sequence is a candidate sequence-specific
DNA-binding protein. Also, SILAC can be
combined with small interfering (si)RNA
knockdown of the antigen of interest in an
immunoprecipitation. By comparison of
eluates of the antibody from cells with and
without siRNA treatment, crossreactivity of
the antibody is eliminated and true interac-
tors of the endogenous protein are obtained28.
SILAC for quantitative phosphoproteomics
MS can directly measure and sequence
endogenous phosphopeptides without
the need to generate special reagents, so it
has therefore become a method of choice
for determining phosphorylation sites
(reviewed in REFS 29–31). Improvements in
phosphoproteomics technology now allow
sequencing of hundreds or even thousands
of phosphorylation sites in a single experi-
ment32. However, it has rapidly become clear
that a functional filter is needed to extract
biological insights from such experiments.
Mapping yeast pheromone responses. SILAC
is ideally suited to distinguish the phos-
phorylation events that are associated with
a particular stimulus from a vast excess of
basal phosphorylation sites. As all peptides
need to be quantifiable, both Arg and Lys
are usually labelled33, and trypsin is used as a
protease. Gruhler et al. labelled a yeast strain
by SILAC and exposed one population to
mating factor and enriched phosphopeptides
from total cell lysate34. Sequencing and quan-
titation of more than 700 phosphopeptides
showed a 2-fold change in 139 of these pep-
tides upon pheromone stimulation. These
phosphorylation sites mapped to proteins in
the entire mating-signalling pathway from
receptors to transcription factors.
Examining stem-cell differentiation.
Quantitative phosphoproteomics can
also directly identify factors that control
cellular decisions. We were interested in
understanding how growth factors influence
stem-cell differentiation, particularly the
differentiation of adult mesenchymal stem
cells to bone-forming cells35. We found that
two related growth factors, EGF and platelet-
derived growth factor (PDGF), induced
Tyr phosphorylation; however, only EGF
strongly enhanced differentiation to
To determine the cause of this difference,
we SILAC-labelled three cell populations
and exposed them to no stimulus, EGF stim-
ulation or PDGF stimulation (FIG. 2). We then
enriched the Tyr phosphoproteome with
anti-phosphotyrosine antibody. As expected,
most of the Tyr phosphoproteome was regu-
lated by both EGF and PDGF, albeit often
to different degrees. However, there were
also a few proteins that were only activated
by one of these growth factors. In particular,
phosphatidylinositol 3-kinase (PI3K)
Figure 1 | Interaction of a bacterial phospho-
protein with a human host protein. An immobi-
lized synthetic phosphopeptide that corresponds
to the phosphorylation site of the bacterial
protein CagA was incubated with heavy stable-
isotope labelling by amino acids in cell culture
(SILAC)-labelled mammalian cell extracts. As a
control, the peptide that lacks the phosphogroup
was incubated with the light-SILAC-labelled cell
extract. Eluates from phosphopeptide and non-
phosphopeptide baits were mixed and analysed
by liquid chromatography–tandem mass spec-
trometry. The proteins that were identified are
aligned according to the logs of their SILAC
ratios. More than 150 proteins were identified
with ratios close to one to one, which shows that
these proteins bind equally well to the peptide,
regardless of the presence of the phosphogroup.
Only SHP2 (Src-homology-2 domain-containing
protein tyrosine phosphatase-2) has a statistically
significant ratio, which indicates that SHP2 binds
specifically to the phosphopeptide. These data
confirm a recently described interaction61.
Figure courtesy of M. Selbach, Max-Planck
Institute for Biochemistry, Martinsried, Germany.
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© 2006 Nature Publishing Group
423 425 427 577 429
ERK2 - FRHENIIGINDIIR
PI3K, p85-β - ILLNSER
8 1676 4 9
Proteins modulated by EGF and/or PDGF
Figure 2 | Systems biology of stem-cell differentiation. a | Mesenchymal adult stem cells differenti-
ated to bone cells upon epidermal growth factor (EGF), but not platelet-derived growth factor (PDGF),
treatment. To determine the proteins that control the differentiation process, stable-isotope labelling
by amino acids in cell culture (SILAC; using Arg0, Arg6 and Arg10) was used to analyse three cell
populations; EGF-treated cells, PDGF-treated cells and control cells that were untreated. Subsequently,
cells were mixed and the phosphotyrosine proteome was enriched using an anti-phosphotyrosine
antibody. b | The heavy-amino-acid SILAC forms of a peptide from mitogen-activated protein kinase-2
(MAPK2, also known as ERK2) from the EGF- and PDGF-stimulated populations are more intense than
the control, which indicates that ERK2 is stimulated by both growth factors. However, a peptide from
the p85β subunit of the phosphatidylinositol 3-kinase (PI3K) is activated over the control (present in
the Tyr phosphoproteome) only in the population that is stimulated by PDGF. This finding indicates
that PI3K might be a possible control point for the differentiation to bone cells. c | Evaluation of the
entire data set showed that 113 proteins are modulated by EGF and/or PDGF (entire oval); 76 proteins
are activated similarly by EGF and PDGF; 16 are more activated by EGF and 4 are more activated by
PDGF (up to threefold); 8 proteins are only activated by EGF, and 9 are only activated by PDGF. LC–MS,
liquid chromatography–mass spectrometry; m/z, mass-to-charge ratio. Adapted with permission from
REF. 35 © (2005) American Association for the Advancement of Science.
Mix lysates 1:1:1
Affinity purification with
Digestion with trypsin
Protein indentification and
quantitation by LC–MS
was only activated by PDGF and was there-
fore a candidate for the differential effects.
Indeed, the PI3K inhibitor wortmannin
caused PDGF stimulation to be as potent in
bone-cell differentiation as EGF in vitro and
in vivo. This finding, which might also be
of clinical interest, shows how quantitative
phosphoproteomics can help to pinpoint sites
of cellular decision making.
The Tyr phosphoproteome has also been
studied in cancer cells. ERBB2 is overex-
pressed in a subset of breast cancers and
phosphotyrosine signalling downstream of
this receptor has been studied by two groups.
Zhang et al. used ligand stimulated versus
unstimulated cells in the SILAC experiment36.
Bose et al. quantified the Tyr phospho-
proteome of untreated and phosphatase-
inhibitor treated ERBB2-overexpressing cells
and used network modelling to account for
the changes in the phosphoproteome37.
Adding the time dimension to proteomics
So far, most proteome experiments char-
acterize a static time point or an on–off
situ ation. To incorporate kinetics into
organellar proteomics, three different cell
populations were labelled, treated with an
inhibitor of transcription and harvested
at three different time points38. Cells were
subsequently mixed and their nucleoli
were isolated. In response to inhibition
of transcription, some proteins leave the
nucleoli, some remain unchanged and some
are recruited to the nucleoli. Previously,
such changes had to be visualized in a
candidate-based approach using GFP-fusion
proteins. By contrast, SILAC directly meas-
ures changes in endogenous proteins in the
entire nucleolar proteome. A peptide from
a protein recruited to the nucleolus would
show a triplet with increasing intensities,
because more of the protein is present in
the nucleolar preparations from later time
points (FIG. 3). By repeating the experiment
several times with a common time point,
up to nine point kinetics were obtained.
Interacting proteins left the nucleolus with
similar time profiles. These time profiles
also helped to group the nucleolar proteome
into interacting modules by machine learn-
ing, which is an area of artificial intelligence
that allows the computer to uncover
patterns and classify data accordingly39.
Once the experiment is set up, SILAC
time courses of a proteome can be obtained
rapidly. Use of different inhibitors of
transcription showed trafficking of dif-
ferent protein populations from or to the
nucleolus. For example, inhibition of the
proteasome resulted in largely opposite
kinetics compared to a specific inhibitor of
polymerase II transcription38. Such findings
can be followed up by further experiments
in conjunction with single-cell fluorescence
microscopy. The combination of modern
microscopy techniques with SILAC is espe-
cially powerful because SILAC is unbiased,
comprehensive and measures endogenous
proteins, whereas biochemical approaches
average over large cell populations. On the
other hand, microscopy supplies detailed
spatial, temporal and interaction information
at the single-cell level, ideally complementing
Another area in which the time dimen-
sion is of great interest is in signalling
cascades. Such kinetics are difficult to obtain
in a global and unbiased way. Using SILAC
and a time-course experiment similar to
the one described for the nucleolus experi-
ment, Blagoev et al. mapped changes in
the phosphotyrosine proteome as a result
of EGF stimulation. Activation profiles
were obtained covering all classes of Tyr
phosphorylated effectors40. Recently, we
have extended this approach to quantita-
tively measure thousands of Ser/Thr/Tyr
phosphoryl ation sites, which yielded a first
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