Article

Efforts toward codon table engineering: expansion of unnatural substrates acceptable by the E. coli ribosome.

Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Nucleic Acids Symposium Series 02/2006; DOI:10.1093/nass/nrl019 pp.37-8
Source: PubMed

ABSTRACT The ribosome catalyzes oligo/polymerization of amino acids. We designed an allowable modification of amino acid backbone, based on the hypothesized mechanism of peptidyl transfer reaction. Nonsense suppression method was used to investigate the acceptability of these substrates in the prokaryotic ribosomal system. The E.coli ribosome showed a restricted tolerance to the backbone modification, especially for main-chain elongation. However, our designed homologous beta-hydroxyalkanoic acid with elongated methylene (backbone) chain-length was revealed to be a possible substrate for the E.coli ribosome.

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Keywords

allowable modification
 
amino acid backbone
 
amino acids
 
backbone modification
 
designed homologous beta-hydroxyalkanoic acid
 
elongated methylene
 
Nonsense suppression method
 
peptidyl transfer reaction
 
prokaryotic ribosomal system
 
restricted tolerance
 
substrates
 

Shinsuke Sando