Article

Effects of carbon concentration and carbon-to-nitrogen ratio on growth and sporulation of several biocontrol fungi

Key Laboratory of Systematic Mycology and Lichenology, Institute of Microbiology, Chinese Academy of Sciences, PO Box 2714, Beijing 100080, PR China.
Mycological Research (Impact Factor: 2.81). 02/2007; 111(Pt 1):87-92. DOI: 10.1016/j.mycres.2006.07.019
Source: PubMed

ABSTRACT Effects of carbon concentration and carbon to nitrogen (C:N) ratio on six biocontrol fungal strains are reported in this paper. All fungal strains had extensive growth on the media supplemented with 6-12 gl(-1) carbon and C:N ratios from 10:1 to 80:1, and differed in nutrient requirements for sporulation. Except for the two strains of Paecilomyces lilacinus, all selected fungi attained the highest spore yields at a C:N ratio of 160:1 when the carbon concentration was 12 gl(-1) for Metarhizium anisopliae SQZ-1-21, 6 gl(-1) for M. anisopliae RS-4-1 and Trichoderma viride TV-1, and 8 gl(-1) for Lecanicillium lecanii CA-1-G. The optimal conditions for P. lilacinus sporulation were 8 gl(-1) carbon with a C:N ratio of 10:1 for M-14 and 12 gl(-1) carbon with a C:N ratio of 20:1 for IPC-P, respectively. The results indicated that the influence of carbon concentration and C:N ratio on fungal growth and sporulation is strain dependent; therefore, consideration for the complexity of nutrient requirements is essential for improving yields of fungal biocontrol agents.

Download full-text

Full-text

Available from: Yongsheng Che, Jul 23, 2015
0 Followers
 · 
288 Views
  • Source
    • "However, there are no data to substantiate this hypothesis and more detailed investigations are necessary to verify a conclusion. Many experiments investigating the influence of culture media on fungal growth have reported that the C:N ratio has a greater impact on growth than the carbon concentration alone [25]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: An investigation of marine red algae surface-associated fungal communities led to the isolation of apreviously undescribed Mucor strain. Many characteristic features of the genus Mucor, including sporangiophores, sporangium, sporangiospores and columellae, were apparent in Scanning Electron Microscope (SEM) images of the isolated strain. The sequence of the internal transcribed spacer (ITS) rDNA revealed that the strain Exhibits 97% homology to the genus Mucor. In a search for active compounds isolated by an ethyl acetate extract of the strain, tyrosol (2-(4-hydroxyphenyl) ethanol) was identified as a major secondary metabolite present in the culture broth. Remarkably, the tyrosol production level was considerably higher than that of reported tyrosol producing microorganisms. The optimal conditions for the fermentative production of tyrosol by the new strain were identified, including culture media, incubation period, temperature and pH. These findings clearly demonstrate that this novel strain has the potential for development as a natural source of tyrosol for industrial purposes.
    PROCESS BIOCHEMISTRY 09/2014; 49(9). DOI:10.1016/j.procbio.2014.06.004 · 2.52 Impact Factor
  • Source
    • "Many nutritional factors are effective in fungal sporulation, e.g., the carbon source, nitrogen source, and microelements (Timnick et al. 1951). Some fungi have specific carbon and nitrogen requirements for sporulation (Engelkes et al. 1997; Gao et al. 2007). Potato dextrose agar (PDA) is the most widely used medium in fungal isolation and culture . "
    [Show abstract] [Hide abstract]
    ABSTRACT: Spore morphologies are a major character in fungal taxonomy, although many isolates are not able to sporulate on common artificial media. This article reviews the effect of nutrition, host tissue, and light on fungal sporulation in artificial media. A trial experiment using 42 strains that failed to sporulate on potato dextrose agar (PDA) and half-strength PDA after 3 months is reported. Five strategies (1/10-strength PDA, CaCO3 water agar, pine needle medium, mulberry agar, and near-ultraviolet light irradiation) were applied to induce these strains to sporulate, with an overall success rate of 62%. Pine needle medium was the most successful method, which induced sporulation of 40% of recalcitrant strains.
    09/2012; 3(3):1-6. DOI:10.1080/21501203.2012.719042
  • Source
    • "Traditional method for sporulation using continuous cultivation with both the vegetative growth and sporulation occur on the same agar plates (Li and Hololdom 1995; Liu and Chen 2002, 2003). But, in fact, the nutritional requirements of vegetative growth and World J Microbiol Biotechnol (2009) 25:1989–1994 1991 sporulation is different (Gao et al. 2007; Sun et al. 2009). Fungi produce more spores under low nutrient conditions but need high nutritional requirements for vegetative growth (Nebane and Ekpo 1992; Elson et al. 1998 "
    [Show abstract] [Hide abstract]
    ABSTRACT: A novel method was described for promoting conidial production of nematophagous fungus, Pochonia chlamydosporia AS6.8, by hydrogen peroxide (H2O2) treatment and two-stage cultivation. The fungi were first grown on potato dextrose agar plates to encourage vegetative growth, after briefly treating with 90mM concentrations of H2O2, and then were transferred to water agar (WA) plates for sporulation. Sporulation of the P. chlamydosporia AS6.8 was significantly enhanced (as much as 15 times) using this method. Enhancement of sporulation by H2O2 (oxidative stress) and by transfer to WA (low-nutrient stress) was not synergistic. In order to make sure if the H2O2 had enhancing effect on other nematophagous fungi, we also evaluated the effect of H2O2 on sporulation of Arthrobotrys oligospora CBS 115.81 and Dactylellina cionopaga CBS 113355 besides P. chlamydosporia. And the result showed that 90mM concentrations of H2O2 had enhancing effect on sporulation of all of the three isolates.
    World Journal of Microbiology and Biotechnology 11/2009; 25(11):1989-1994. DOI:10.1007/s11274-009-0099-y · 1.35 Impact Factor
Show more