Neural crest-derived cells with stem cell features can be traced back to multiple lineages in the adult skin. J Cell Biol

Department of Biology, Institute of Cell Biology, Swiss Federal Institute of Technology ETH Zurich, CH-8093 Zurich, Switzerland.
The Journal of Cell Biology (Impact Factor: 9.69). 01/2007; 175(6):1005-15. DOI: 10.1083/jcb.200606062
Source: PubMed

ABSTRACT Given their accessibility, multipotent skin-derived cells might be useful for future cell replacement therapies. We describe the isolation of multipotent stem cell-like cells from the adult trunk skin of mice and humans that express the neural crest stem cell markers p75 and Sox10 and display extensive self-renewal capacity in sphere cultures. To determine the origin of these cells, we genetically mapped the fate of neural crest cells in face and trunk skin of mouse. In whisker follicles of the face, many mesenchymal structures are neural crest derived and appear to contain cells with sphere-forming potential. In the trunk skin, however, sphere-forming neural crest-derived cells are restricted to the glial and melanocyte lineages. Thus, self-renewing cells in the adult skin can be obtained from several neural crest derivatives, and these are of distinct nature in face and trunk skin. These findings are relevant for the design of therapeutic strategies because the potential of stem and progenitor cells in vivo likely depends on their nature and origin.

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Available from: Thomas Pietri, Aug 26, 2015
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    • "Compared with the in vivo stripped Descemet's membrane, the resultant cell shape remained hexagonal during the entire knockdown period (5 wk of weekly treatment after the first week of culture followed by withdrawal for 1 wk) for HCEC monolayers treated with scRNA or p120-Kaiso siRNAs (Fig. 6 A). The resultant cell density was also maintained at the in vivo level by p120-Kaiso siRNAs in MESCM, but was decreased, however, by scRNA after 6 wk of culturing (Fig. 6 B). 1 wk after withdrawal, the resultant HCEC monolayer retained the centric expression of acetylate--catenin (a marker of the basal body and the primary cilium; Blitzer et al., 2011), uniform cytoplasmic expression of -tubulin and p75NTR (of which the latter is considered a marker of neural crest cells; Wong et al., 2006), and junctional expression of p120, N-cadherin, -catenin, -catenin, Zona occludens protein 1 (ZO-1), Na-K-ATPase, and F-actin (all are markers of HCECs), without expression of LEF1 and S100A4 (both are markers of EMT; Zhu et al., 2012; Fig. 6 C). Such expression of HCEC markers was consistent with the in vivo expression pattern previously reported by us (Zhu et al., 2012, 2014). "
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    • "Whisker follicles are known to serve as a local reservoir of mast cell precursors as it was shown by others that the middle part of dissected whisker follicles is able to produce mast cells in culture when stimulated with the appropriate growth and differentiation factors (Kumamoto et al., 2003). In addition, whisker follicle mesenchymal stem cells, like the cranial neural crest cells from which they are derived, can differentiate into adipocytes in vitro (Wong et al., 2006; Billon et al., 2008). "
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    • "The mammalian hair follicle contains several compartments of stem cells that are responsible for regeneration of these structures during the anagen phase of the hair follicle as well as after injury (for review, see Fuchs and Horsley, 2008; Tiede et al., 2007). A cell population of neural crest origin has been identified in the hair follicle bulge region by the expression of b-galactosidase expression under the Wnt1 promoter in transgenic mouse models (Nagoshi et al., 2008; Sieber-Blum et al., 2004; Wong et al., 2006). These HFNCSCs express neural crest markers Sox10 and neural crest/neural stem cell marker nestin and can be differentiated toward neural crest lineages such as neurons, smooth muscle cells, and melanocytes in vitro (Fernandes et al., 2004; "
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