Article

Cloning and characterization of a cDNA encoding a cowpea seed defensin and analysis of its expression.

Laboratório de Fisiologia e Bioquímica de Microrganismos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes - RJ, Brazil.
Protein and Peptide Letters (impact factor: 1.94). 02/2006; 13(10):1029-36. pp.1029-36
Source: PubMed

ABSTRACT We have previously isolated a cowpea seed defensin with potent antifungal activity. Here we report the cloning of its cDNA obtained from RT-PCR. The sequence of the deduced protein VUDEF exhibited homology to plant defensins. Northern analysis revealed that VUDEF mRNA is accumulated during cowpea seed development and its level increases in seedling tissues after exposure to fungal pathogen and cold.

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Keywords

cowpea seed defensin
 
cowpea seed development
 
deduced protein VUDEF exhibited homology
 
level increases
 
Northern analysis
 
potent antifungal activity
 
RT-PCR
 
seedling tissues