High-throughput functional genomic methods to analyze the effects of dietary lipids

Laboratory for Functional Genomics, Biological Research Center of the Hungarian Academy of Sciences, Temesvari krt. 62. Szeged, H-6726, Hungary.
Current pharmaceutical biotechnology (Impact Factor: 2.51). 01/2007; 7(6):525-9. DOI: 10.2174/138920106779116801
Source: PubMed

ABSTRACT The applications of 'omics' (genomics, transcriptomics, proteomics and metabolomics) technologies in nutritional studies have opened new possibilities to understand the effects and the action of different diets both in healthy and diseased states and help to define personalized diets and to develop new drugs that revert or prevent the negative dietary effects. Several single nucleotide polymorphisms have already been investigated for potential gene-diet interactions in the response to different lipid diets. It is also well-known that besides the known cellular effects of lipid nutrition, dietary lipids influence gene expression in a tissue, concentration and age-dependent manner. Protein expression and post-translational changes due to different diets have been reported as well. To understand the molecular basis of the effects and roles of dietary lipids high-throughput functional genomic methods such as DNA- or protein microarrays, high-throughput NMR and mass spectrometry are needed to assess the changes in a global way at the genome, at the transcriptome, at the proteome and at the metabolome level. The present review will focus on different high-throughput technologies from the aspects of assessing the effects of dietary fatty acids including cholesterol and polyunsaturated fatty acids. Several genes were identified that exhibited altered expression in response to fish-oil treatment of human lung cancer cells, including protein kinase C, natriuretic peptide receptor-A, PKNbeta, interleukin-1 receptor associated kinase-1 (IRAK-1) and diacylglycerol kinase genes by using high-throughput quantitative real-time PCR. Other results will also be mentioned obtained from cholesterol and polyunsaturated fatty acid fed animals by using DNA- and protein microarrays.

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Available from: László G Puskás, Sep 26, 2015
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    • "cancer and transcriptomics has used to study intestinal biology (Ross 2007; Culhane and Howlin 2007; Fleet 2007; Schäfer et al., 2007 ). Interrelationship between the food components and the 'omics techniques' (transcriptomics, proteomics, genomics, epigenomics and metabolomics) have a significant influence on the quality of life as measured by both physical and cognitive performance and modify the risk and severity of a variety of cancer diseases conditions (Milner 2007; He 2006; Puskás et al., 2006; Fan et al., 2006; Miles et al., 2006; Davis and Milner 2004). "
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