Sterilization method of platelet storage containers affects in vitro parameters

Sanquin Blood Bank North-West Region, Amsterdam, The Netherlands.
Vox Sanguinis (Impact Factor: 3.3). 01/2007; 92(1):32-6. DOI: 10.1111/j.1423-0410.2006.00855.x
Source: PubMed

ABSTRACT Four butyryl trihexyl phthalate plasticized polyvinyl chloride (BTHC-PVC) containers were compared for storage of leukoreduced platelet concentrates (LR-PC): three ethylene oxide (EtO) sterilized (Gambro, Haemonetics, Fresenius), and one steam-sterilized (Fresenius).
LR-PCs were made from 5 buffy coats and 300 ml Composol additive solution, and leukoreduced by filtration. Four LR-PCs were pooled and subsequently divided over the 4 BTHC-PVC bags to prevent donor-dependent differences, and sampled for in vitro analysis on day 1, 2, 5, 7 and 9.
The pH values on day 9 were (mean +/- SD, n = 10): 7.12 +/- 0.03 (Gambro), 7.12 +/- 0.04 (Haemonetics), and 7.07 +/- 0.09 (Fresenius, EtO-sterilized) (not significantly different), vs. 6.91 +/- 0.12 (Fresenius, steam-sterilized; P < 0.001 vs. all EtO-sterilized bags). LR-PCs stored in the steam-sterilized bag exhibited significantly higher glucose consumption and lactate production (P < 0.001 vs. all EtO-sterilized bags).
All BTHC-PVC containers allow storage of LR-PCs for up to 9 days with good in vitro parameters. However, the method of sterilization affects the storage conditions of the LR-PCs in these bags.

  • [Show abstract] [Hide abstract]
    ABSTRACT: To evaluate the post-storage viability of apheresis platelets stored for up to 18 days in 80% PAS/20% plasma, 117 healthy subjects donated platelets using the Haemonetics MCS+, COBE Spectra (Spectra), or Trima Accel (Trima) systems. Control platelets from the same subjects were compared to their stored test PAS platelets by radiolabeling their stored and control platelets with either (51)Chromium or (111)Indium. Trima platelets met FDA post-storage platelet viability criteria for only 7 days versus almost 13 days for Haemonetics platelets; i.e., platelet recoveries after these storage times averaged 44 ± 3% versus 49 ± 3% and survivals 5.4 ± 0.3 days versus 4.6 ± 0.3 days, respectively. The differences in storage duration are likely related to both the collection system as well as the storage bag. The Spectra and Trima platelets were hyperconcentrated during collection and PAS was added, while the Haemonetics platelets were elutriated with PAS which may have resulted in less collection injury. When Spectra and Trima platelets were stored in Haemonetics' bags, post-storage viability was significantly improved. Platelet viability is better maintained in vitro than in vivo allowing substantial increases in platelet storage times. However, implementation will require resolution of potential bacterial overgrowth during storage.
    Blood 11/2013; 123(2). DOI:10.1182/blood-2013-05-501247 · 9.78 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Apheresis platelet (PLT) units are not routinely agitated during transit. Our study compared the in vitro properties of apheresis PLT units that were stored with continuous agitation (CA) and without continuous agitation (WCA) during two separate periods, immediately after collection and between Day 2 and Day 3 of storage. Two identical apheresis PLTs units were prepared from collections with Amicus (n = 11, Fenwal, Inc.) and Trima (n = 10, CaridianBCT) cell separators. One apheresis PLT unit was continuously agitated, starting routinely within 30 minutes of collection, and an identical apheresis PLT unit was held without agitation initially for 7 to 8 hours and subsequently for 24 hours between Day 2 and Day 3 of storage. The apheresis PLT units were maintained WCA at 20 to 24 °C in a shipping box. In vitro PLT properties were evaluated on Day 1 (day after collection), after 5 and 7 days of storage. With both Amicus and Trima apheresis PLT units, the mean PLT content and concentration of CA and WCA were comparable and essentially constant throughout storage. Mean pH levels (± 1 SD) after 5 days for Amicus apheresis PLT units were 6.97 ± 0.20 (WCA) and 7.13 ± 0.16 (p < 0.001, CA) and for Trima apheresis PLT units 6.97 ± 0.21 (WCA) and 7.22 ± 0.17 (p < 0.001, CA). In vitro variables, including percentage of disc PLTs, extent of shape change, and hypotonic stress levels, after 5 days of storage, showed mean differences between WCA and CA that were less than 15%. The in vitro results show that apheresis PLT units can be stored without agitation for 7 to 8 hours immediately after collection and also subsequently during storage for 24 hours with minimal influence on in vitro PLT properties compared to continuously agitated PLTs.
    Transfusion 03/2011; 51(3):636-42. DOI:10.1111/j.1537-2995.2010.02869.x · 3.57 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The novel TACSI system is designed for automated preparation of platelets (PLTs) from pooled buffy coats (BCs). One TACSI device will handle 6 units at the same time. The aim of our in vitro study is to investigate the effects of using this automated equipment with subsequent storage in two different plastic containers and to compare these results with PLTs prepared by the OrbiSac system. Buffy-coat-derived PLTs (n=8) were prepared by using the TACSI system, including storage in polyvinyl chloride (PVC)-based plastic containers with di, n-decyl phthalate (DnDP) (TACSI R) and BTHC (TACSI T)-based plasticizers. As a reference, the OrbiSac System was used to prepare PLTs (n=8) with subsequent storage in a PVC plastic container with a citrate-based plasticizer (BTHC). In total, 16 TACSI and eight reference units, supplied by approximately 30% plasma and 70% SSP+, were analysed for various in vitro variables during the 7-day storage period. No significant difference in PLT counts, LDH, mean platelet volume (MPV) and adenosine triphosphate between the groups was detected. Glucose was lower (P<0·05) and lactate was higher (P<0·05) in TACSI R vs. OrbiSac. With exception of day 7 (P<0·05 TACSI R vs. OrbiSac), HSR reactivity were not different between groups. Extent of shape change was lower and CD62P higher in TACSI T when compared with TACSI R and OrbiSac units (P<0·05). pH was maintained at >6·8 (day 7) and swirling remained at the highest level (score=2) for all units throughout storage. Platelets prepared by the TACSI system with subsequent storage in two different PVC-based plastic containers were equivalent to reference PLTs with regard to in vitro characteristics during 7 days of storage.
    Vox Sanguinis 11/2010; 99(4):341-7. DOI:10.1111/j.1423-0410.2010.01364.x · 3.30 Impact Factor

Full-text (2 Sources)

Available from
Mar 17, 2015