Extract of Pelargonium sidoides (EPs® 7630) improves phagocytosis, oxidative burst, and intracellular killing of human peripheral blood phagocytes in vitro

Institute of Environmental Medicine and Hospital Epidemiology, University Medical Center Freiburg, Freiburg, Germany.
Phytomedicine (Impact Factor: 3.13). 03/2007; 14 Suppl 6(Suppl. 6):46-51. DOI: 10.1016/j.phymed.2006.11.016
Source: PubMed


Clinical data show that EPs® 7630, an aqueous ethanolic extract from the roots of Pelargonium sidoides, can be used for the treatment of upper respiratory tract infections (URTI). The biological effects of the preparation have not been fully investigated. The objective of this study was to examine the impact of EPs® 7630 on the activity of human peripheral blood phagocytes (PBP). A whole blood-based, flow cytometric assay was used to simultaneously assess phagocytosis and oxidative burst. EPs® 7630 was applied in therapeutically relevant concentrations between 0 and 30 ?g/ml. Compared with controls EPs® 7630 increased the number of phagocytosing PBP in a concentration-dependent manner, with a maximum enhancement of 56% at 2 min. The application of EPs® 7630 also led to a significant increase in the number of burst-active PBP for all time points observed beyond 2 min. Using a microbiological assay, intracellular killing was also enhanced by EPs® 7630. In conclusion, the positive effects of EPs® 7630 on phagocytosis, oxidative burst, and intracellular killing of yeast cells as test organisms are important components of the compound's biological activity. Our findings constitute a valuable contribution to understanding the clinical effects of EPs® 7630.

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    • "Pelargonium sidoides is a traditional medicinal plant of South Africa and Lesotho that has been widely evaluated for its use in the treatment of upper respiratory tract infections (Matthys et al., 2003, 2007; Chuchalin et al., 2005; Lizogub et al., 2007). The beneficial effects of P. sidoides can be attributed to its antiviral, antibacterial and immunomodulatory activities (Kayser and Kolodziej, 1997; Kayser et al., 2001; Kolodziej et al., 2003; Koch et al., 2006; Lewu et al., 2006; Mativandlela et al., 2006; Conrad et al., 2007; Noldner and Schotz, 2007; Schnitzler et al., 2008: Michaelis et al., 2011). Extracts are prepared from the mature red roots of this plant. "
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    ABSTRACT: Extracts prepared from the roots of Pelargonium sidoides (DC) are commercially available for the treatment of respiratory related conditions. Recently, a commercial radix mother tincture of this plant was shown to have both antioxidant and anticancer effects especially related to the G 0/G 1 block in the Jurkat E6.1 cell line (unpublished results). Fractions were prepared by semi-preparative HPLC, and their antioxidant and anticancer activities were determined. The more hydrophilic fractions isolated namely F6-F12 were all found to have strong reducing capacities and were able to scavenge peroxyl radicals. In the human lung cell line, NCI-H460, significant cellular antioxidant effects were observed. Anticancer activity was evaluated in the NCI-pre-screen panel (NCI-H460, MCF-7 and SF-268) and the Jurkat E6.1 cell line. Fractions F7, F9, and F12 were found to inhibit the cell growth of these four cell lines (p < 0.05), especially the Jurkat E6.1 cell line with the sulforhodamine B assay. Mass spectrometry analysis revealed that these active fractions contained several polyphenolic compounds such as gallic acid, trihydroxycoumarin, dihydroxycoumarin sulfates, proanthocyanidins, and phenolic glycosides. A phenolic acid glycoside sulfate not previously shown in P. sidoides extracts was also isolated. In conclusion, the antioxidant and/or anticancer activity of the P. sidoides tincture may be attributed to the presence of these polyphenolics.
    Medicinal Chemistry Research 11/2015; 24(11). DOI:10.1007/s00044-015-1425-6 · 1.40 Impact Factor
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    • "No serious adverse events were reported. EPs 7630 has a positive effect on phagocytosis, oxidative burst, and intracellular killing of cells [120–125]. P. sidoides extract modulates the production of secretory immunoglobulin A in saliva, both interleukin-15 and interleukin-6 in serum, and interleukin-15 in the nasal mucosa [19, 126]. "
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    ABSTRACT: The use of medicinal plants as a fundamental component of the African traditional healthcare system is perhaps the oldest and the most assorted of all therapeutic systems. In many parts of rural Africa, traditional healers prescribing medicinal plants are the most easily accessible and affordable health resource available to the local community and at times the only therapy that subsists. Nonetheless, there is still a paucity of updated comprehensive compilation of promising medicinal plants from the African continent. The major focus of the present review is to provide an updated overview of 10 promising medicinal plants from the African biodiversity which have short- as well as long-term potential to be developed as future phytopharmaceuticals to treat and/or manage panoply of infectious and chronic conditions. In this endeavour, key scientific databases have been probed to investigate trends in the rapidly increasing number of scientific publications on African traditional medicinal plants. Within the framework of enhancing the significance of traditional African medicinal plants, aspects such as traditional use, phytochemical profile, in vitro, in vivo, and clinical studies and also future challenges pertaining to the use of these plants have been explored.
    Evidence-based Complementary and Alternative Medicine 12/2013; 2013(2):617459. DOI:10.1155/2013/617459 · 1.88 Impact Factor
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    • "All rights reserved. doi:10.1016/j.phymed.2010.08.003 on the respiratory tract mucosa (Bachert et al. 2009; Conrad et al. 2007). "
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    ABSTRACT: The evidence that exhaustive exercise may compromise the immune response is mainly confirmed by upper respiratory tract infections which are probably related to the decrease in secretory immunoglobulin A in the upper airway mucosa and/or profile changes of systemic cytokines as well as local cytokines of the upper respiratory tract. An extract from Pelargonium sidoides roots is currently used to treat infections in the upper airways. The aim of the present study was to evaluate the action of this herbal medicine on the immune response of athletes submitted to an intense running session by analyzing the production of immunoglobulin A in their saliva and of cytokines both locally and systemically, using a placebo as control. The results show that Pelargonium sidoides extract modulates the production of secretory immunoglobulin A in saliva, both interleukin-15 and interleukin-6 in serum, and interleukin-15 in the nasal mucosa. Secretory immunoglobulin A levels were increased, while levels of IL-15 and IL-6 were decreased. Based on this evidence, we suggest that this herbal medicine can exert a strong modulating influence on the immune response associated with the upper airway mucosa in athletes submitted to intense physical activity.
    Phytomedicine: international journal of phytotherapy and phytopharmacology 02/2011; 18(4):303-8. DOI:10.1016/j.phymed.2010.08.003 · 3.13 Impact Factor
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