Stir bar sorptive extraction and liquid chromatography with UV detection for determination of antidepressants in plasma samples
ABSTRACT A sensitive and reproducible stir bar sorptive extraction and liquid chromatography (SBSE/LC-UV) method is described for the determination of sertraline, mirtazapine, fluoxetine, citalopram, paroxetine, imipramine, nortriptyline, amitriptyne, and desipramine in plasma samples. Important factors in the optimization of SBSE efficiency are discussed, such as extraction time, pH, ionic strength, influence of plasma proteins, and desorption conditions: solvents, modes (magnetic stir, ultrasonic), time, and number of desorption steps. The SBSE/LC-UV method showed to be linear in a concentration ranging from the limit of quantification (LOQ) to 1000.0 ng mL(-1). The LOQ values ranged from 10.0 ng mL(-1) to 40.0 ng mL(-1). The inter-day precision of the SBSE/LC-UV method presented coefficient of the variation lower than 15%. Based on figures of the merit results, the SBSE/LC-UV methodology showed to be adequate to the antidepressants analyses from therapeutic to toxic therapeutic levels. In order to evaluate the proposed method for clinical use, the SBSE/LC-UV method was applied to the analysis of plasma samples from elderly depressed patients.
- SourceAvailable from: Zarrin Es'haghi
[Show abstract] [Hide abstract]
- "www.sciencedirect.com consuming and require large amounts of organic solvents . "
ABSTRACT: This paper describes a new approach for the determination of amitriptyline in wastewater by ionic liquid based immersed droplet microextraction (IL-IDME) prior to high-performance liquid chromatography with ultraviolet detection. 1-Hexyl-3-methylimidazolium hexafluorophosphate ([C6MIM][PF6]) was used as an ionic liquid. Various factors that affect extraction, such as volume of ionic liquid, stirring rate, extraction time, pH of the aqueous solution and salting effect, were optimized. The optimal conditions were as follows: microextraction time, 10 min; stirring rate, 720 rpm; pH, 11; ionic drop volume, 100 μL; and no sodium chloride addition. In quantitative experiments the method showed linearity in a range from 0.01 to 10 μg/mL, a limit of detection of 0.004 μg/mL and an excellent pre-concentration factor (PF) of 1100. Finally, the method was successfully applied to the determination of amitriptyline in the hospital wastewater samples.10/2012; 2(5):361–365. DOI:10.1016/j.jpha.2012.07.007
[Show abstract] [Hide abstract]
- "Stationary phase development continues to be an issue, and Collins's group is currently developing immobilized and monolithic stationary phases (Faria et al. 2007; Faria et al. 2006) and Cass's group is developing Restricted Access Medium (RAM) columns (Cassiano et al. 2006; Lima et al. 2006). Advances in hardware for hyphenated techniques encompassing HPLC and supercritical fluid chromatography have been undertaken by Lanças et al. more recently focusing on Solid Phase Extraction (SPE) techniques (Chaves et al. 2007; Fernandes et al. 2007). He also started the successful COLACRO Latin-American Chromatography Congresses. "
ABSTRACT: This review highlights some relevant contributions proposed by Brazilian analytical research groups mainly after 2004. This year was chosen due to an earlier publication of an overview with similar scope by Andrade et al. (2004). The selected papers are representative, but the authors have not tried to present a comprehensive overview of the aforementioned period. The chosen areas for discussion were automation in analytical chemistry, chemometrics, chromatography, capillary electrophoresis and mass spectrometry, electroanalytical chemistry, environmental analytical chemistry, preconcentration and separation procedures, and spectrochemical analysis. A glimpse of the Brazilian contributions shows the stage of development of analytical chemistry in Brazil, with emphasis on major advancements and critical needs.Analytical Letters 07/2008; 41(9):1494-1546. DOI:10.1080/00032710802136289 · 0.98 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: Stir bar sorptive extraction (SBSE) is an extraction technique for enrichment of volatile and semi-volatile organic compounds from aqueous and gaseous media. After exposure to a sample, the stir bar, which is covered in a layer of a polysiloxane is subsequently removed and the sorbed compounds are then either thermally desorbed, and analysed by GC-MS or desorbed by means of a liquid, for improved selectivity or for interfacing to an LC system.The technique has been applied successfully to trace analysis in environmental, biomedical and food applications. Applications of SBSE to environmental, foodstuffs and pharmaceutical and biomedical samples are given.Chromatographia 05/2008; 69:79-94. DOI:10.1365/s10337-008-0687-2 · 1.37 Impact Factor