Article

Characterization of superlighting polymer-DNA aggregates: a fluorescence and light scattering study.

Department of Chemistry, Centre d'optique, photonique et laser (COPL), and Centre de recherches sur la science et l'ingénierie des macromolécules (CERSIM), Université Laval, Quebec City, QC, Canada.
Langmuir (impact factor: 4.19). 02/2007; 23(1):258-64. DOI:10.1021/la061699a pp.258-64
Source: PubMed

ABSTRACT The massive amplification of fluorescence signal observed upon hybridization of as few as five DNA molecules into self-assembled structures formed between a cationic polymer and DNA oligonucleotides is investigated. These superlighting polymer-DNA aggregates were studied by fluorescence spectroscopy, static and dynamic light scattering, and zeta potential measurements in order to characterize the aggregation behavior and to understand the processes involved during DNA detection. Multi-angle laser light scattering was also used to obtain the weight-average aggregate mass (AM), the aggregation number (Nagg), the radius of gyration (Rg), and the dissymmetry ratio (z). These results have been used, together with TEM imaging, to propose a suitable physical model for the aggregates.

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Keywords

aggregates
 
aggregation number
 
cationic polymer
 
DNA detection
 
DNA oligonucleotides
 
five DNA molecules
 
fluorescence signal
 
fluorescence spectroscopy
 
gyration
 
massive amplification
 
Multi-angle laser light scattering
 
processes
 
static
 
suitable physical model
 
superlighting polymer-DNA aggregates
 
TEM imaging
 
weight-average aggregate mass
 
zeta potential measurements
 

Kim Doré