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Activities of four frog skin-derived antimicrobial peptides (temporin-1DRa, temporin-1Va and the melittin-related peptides AR-23 and RV-23) against anaerobic bacteria

Institute of Medical Microbiology and Immunology, University of Pécs, Fuenfkirchen, Baranya, Hungary
International Journal of Antimicrobial Agents (Impact Factor: 4.26). 04/2007; 29(3):317-21. DOI: 10.1016/j.ijantimicag.2006.09.007
Source: PubMed

ABSTRACT The activities of two antimicrobial peptides belonging to the temporin family (temporin-1DRa from Rana draytonii and temporin-1Va from Rana virgatipes) and two peptides with structural similarity to the bee venom peptide melittin (AR-23 from Rana tagoi and RV-23 from R. draytonii) were evaluated against a range of reference strains and clinical isolates of anaerobic bacteria. These peptides were selected because they show broad-spectrum growth inhibitory activity against reference strains of several medically important aerobic microorganisms and against clinical isolates of methicillin-resistant Staphylococcus aureus. All peptides showed relatively high potency (minimum inhibitory concentration (MIC) </=25 microM) against the Gram-positive bacilli Propionibacterium acnes and Clostridium tertium and the Gram-positive cocci Peptostreptococcus anaerobius. Activity was lower and more variable against Clostridium septicum, Clostridium perfringens and Peptostreptococcus asaccharolyticus. Growth of the Gram-negative bacilli Bacteroides fragilis and Fusobacterium spp. was poorly inhibited, but all the peptides were active (MIC</=25 microM) against Prevotella melaninogenica. The clinical utility of the melittin-related peptides is limited by their toxicities, but temporin-1DRa and temporin-1Va have relatively low haemolytic activity against human erythrocytes and so represent candidates for drug development, particularly for topical therapy of infected surface lesions.

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    • "In vitro growth inhibition assays have been widely used to determine the effectiveness of skin secretions and isolated peptides against a range of different microorganisms (Rollins- Smith et al., 2002a; Urban et al., 2007; Mangoni et al., 2008; Sheafor et al., 2008). The skin secretions of Leiopelmatid species contain a variety of novel peptides and compounds with anti-chytrid properties, which are in the process of being identified (S. "
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