Use of the diffusive gradients in thin films technique to evaluate (bio)available trace metal concentrations in river water

Institute of Chemistry and Technology of Environmental Protection, Brno University of Technology, Faculty of Chemistry, Purkynova 118, 61200, Brno, Czech Republic.
Analytical and Bioanalytical Chemistry (Impact Factor: 3.44). 04/2007; 387(6):2239-44. DOI: 10.1007/s00216-006-0996-y
Source: PubMed


Concentrations of Cd, Cu, Cr, Pb, Ni and Zn were monitored in the Svitava River (the Czech Republic) during April and September 2005. Total concentrations and total dissolved concentrations were obtained through regular water sampling, and the diffusive gradients in thin films technique (DGT) were used to gain information on the kinetically labile metal concentrations. Each measured concentration was compared with the corresponding average (bio)available concentration calculated from the mass of metal accumulated by the moss species Fontinalis antipyretica. The concentrations of Cd, Pb, Cr and Zn measured using DGT corresponded well with those obtained after the deployment of Fontinalis antipyretica moss bags in the Svitava River, but the concentrations of Cu and Ni did not. The calculated (bio)available Cu concentration correlated well with the total dissolved concentration of Cu, whereas no correlation was found to exist between the concentrations of Ni.

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    • "In particular, the DGT is expected to sample preferentially free metals and easily dissociating metal inorganic complexes (i.e., the so-called 'labile' fraction) and to exclude metal–humic and fulvic acid complexes or to reduce their diffusion velocity through the gel (Zhang and Davison, 2000). DGT is thus expected to account for the complexation processes occurring in water and to sample preferentially species that are biologically relevant for aquatic organisms (Meylan, 2003; Tusseau-Vuillemin et al., 2004; Diviš et al., 2007). Nevertheless, as widely demonstrated in studies focused on BLM developments (Di Toro et al., 2001; De Schamphelaere and Janssen, 2002), metal complexation is only one of the pillars explaining bioavailability. "
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    ABSTRACT: The present report is the companion study of our previous study in which we investigated the impact of the dissolved organic matter, water cationic composition and pH on the bioavailability and the bioaccumulation of copper (Cu) in aquatic mosses (Fontinalis antipyretica). The impact had been assessed under laboratory controlled conditions and modelled using a two-compartment model calibrated under a wide range of water compositions (Ferreira et al., 2008, 2009). Herein are reported the validation stage of the abovementioned approach for contrasted geochemical field conditions. Experiments were performed with aquatic mosses that were exposed for 7d to two nominal Cu concentrations (5 and 15μgL(-1)) in a flow-through field microcosm supplied with four contrasting natural waters. At the end of the exposure period, a 6-fold difference in the bioaccumulated Cu contamination levels was found among the four deployment sites, suggesting a significant control of the water quality on the metal bioaccumulation by aquatic mosses. In parallel, the so-called 'labile' Cu concentration for the same four field conditions was determined using a DGT device (Diffusive Gradient in Thin film). By coupling these DGT measurements and a cation competition model involving Ca(2+), Mg(2+), Na(+) and H(+), the time-dependent Cu concentrations in aquatic mosses were predicted; these simulation results were compared to the actual bioaccumulation of Cu in mosses. We found that any bioaccumulation model that ignores water characteristics is not suitable to predict the Cu accumulation by aquatic mosses under various water quality conditions. Instead, we found that our approach integrating DGT measurements and cationic composition was able to reproduce the Cu bioaccumulation kinetics by aquatic mosses for a wide range of water quality conditions. In conclusion, the DGT approach was demonstrated to be a dynamic in situ measuring technique that can be used as a surrogate of bioindicators if the cationic correction is taken into account.
    Chemosphere 01/2013; 91(3). DOI:10.1016/j.chemosphere.2012.10.016 · 3.34 Impact Factor
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    • "One of the most successful bioaccumulation techniques is that based on aquatic bryophytes, native or transplanted, whose intense and widespread deployment in several European watercourses since the 1970's (Besse et al., 2012; Burton, 1990; Tyler, 1990) is very promising for both surveillance and investigative monitoring, even in aggressive environments such as effluents (Figueira and Ribeiro, 2005; Mersch and Reichard, 1998). A recent study carried out by Divis et al. (2006) proved that the concentrations of Cd, Pb, Cr and Zn measured using D.G.T. (passive samplers) correspond well with those obtained after the deployment of Fontinalis antipyretica transplanted along the same watercourse. "
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    ABSTRACT: An innovative network based on transplanted bryophytes providing a continuous monitoring of the priority substances Cd, Hg, Ni, and Pb and other trace elements (Co, Cr, Cu, Fe, Mn, Zn) was designed for the watercourses flowing across an industrial district of NE Italy where both permitted and illegal wastes cause sporadic, intermittent or chronic events of environmental alteration. During a two-year preliminary survey, over 300 biomonitoring actions ('moss bag' transplantation and recovery) were successfully carried out at 25 stations: 190 of them occurred under acceptable conditions and provided results suitable for comparisons. Five environmental priorities were assessed and characterized in space and time. For these situations local authorities drafted a protocol for data management, to plan official controls and dissuasive actions. The 'moss bag' technique allows a flexible approach for both surveillance monitoring (trend assessment) and investigations (point source detection) in compliance with the Water Framework Directive 2000/60/EC as suggested by the recent Guidance Document on chemical monitoring of sediment and biota.
    Journal of Environmental Management 11/2012; 114. DOI:10.1016/j.jenvman.2012.10.022 · 2.72 Impact Factor
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    • "Buzier et al. (2006) also observed toxic concentrations in labile copper (EC50 lab ) in the both raw and treated effluent of the same WWTP higher than those obtained in inorganic media. Other studies have also shown that, depending on the nature of DOM, DGT measurements could overestimate copper toxicity (Luider et al., 2004; Tusseau-Vuillemin et al., 2004; Divis et al., 2007). "
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    ABSTRACT: A combination of reverse osmosis (RO) concentration and DAX-8/XAD-4 resin adsorption techniques is used to isolate the various constituents of urban dissolved organic matter (DOM) from inorganic salts. Three fractions: hydrophobic (HPO), transphilic (TPI) and hydrophilic (HPI) accounting respectively for 35%, 20% and 45% of extracted carbon, are isolated from effluents of a major French wastewater treatment plant. This atypical DOC distribution, in comparison with natural water where the HPO fraction dominates, shows the significance of HPI fraction which often gets neglected because of extraction difficulties. A number of analytical techniques (elemental, spectroscopic: UV, FTIR) allow highlighting the weak aromaticity of wastewater effluent DOM (EfOM) due to fewer degradation and condensation processes and the strong presence of proteinaceous structures indicative of intense microbial activity. Copper toxicity in the presence of DOM is estimated using an acute toxicity test on Daphnia Magna (Strauss). Results reveal the similar protective role of each EfOM fraction compared to reference Suwannee river fulvic acid despite lower EfOM aromaticity (i.e. specific UV absorbance). The environmental implications of these results are discussed with respect to the development of site-specific water quality criteria.
    Chemosphere 09/2008; 73(4):593-9. DOI:10.1016/j.chemosphere.2008.05.064 · 3.34 Impact Factor
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