Total and functional parasite specific IgE responses in Plasmodium falciparum-infected patients exhibiting different clinical status

Instituto Gulbenkian de Ciencia, LEA CNRS-IGC, Oeiras, Portugal. <>
Malaria Journal (Impact Factor: 3.11). 02/2007; 6(1):1. DOI: 10.1186/1475-2875-6-1
Source: PubMed


There is an increase of serum levels of IgE during Plasmodium falciparum infections in individuals living in endemic areas. These IgEs either protect against malaria or increase malaria pathogenesis. To get an insight into the exact role played by IgE in the outcome of P. falciparum infection, total IgE levels and functional anti-parasite IgE response were studied in children and adults, from two different endemic areas Gabon and India, exhibiting either uncomplicated malaria, severe non cerebral malaria or cerebral malaria, in comparison with control individuals.
Blood samples were collected from controls and P. falciparum-infected patients before treatment on the day of hospitalization (day 0) in India and, in addition, on days 7 and 30 after treatment in Gabon. Total IgE levels were determined by ELISA and functional P. falciparum-specific IgE were estimated using a mast cell line RBL-2H3 transfected with a human Fcepsilon RI alpha-chain that triggers degranulation upon human IgE cross-linking. Mann Whitney and Kruskall Wallis tests were used to compare groups and the Spearman test was used for correlations. Total IgE levels were confirmed to increase upon infection and differ with level of transmission and age but were not directly related to the disease phenotype. All studied groups exhibited functional parasite-specific IgEs able to induce mast cell degranulation in vitro in the presence of P. falciparum antigens. Plasma IgE levels correlated with those of IL-10 in uncomplicated malaria patients from Gabon. In Indian patients, plasma IFN-gamma , TNF and IL-10 levels were significantly correlated with IgE concentrations in all groups.
Circulating levels of total IgE do not appear to correlate with protection or pathology, or with anti-inflammatory cytokine pattern bias during malaria. On the contrary, the P. falciparum-specific IgE response seems to contribute to the control of parasites, since functional activity was higher in asymptomatic and uncomplicated malaria patients than in severe or cerebral malaria groups.

Download full-text


Available from: Joana Nunes Duarte,
  • Source
    • "The main function of IgEs is providing immune defense against parasites such as helminthes and protozoa ( Erb , 2007 ; Duarte et al . , 2007 ) . It was highly unexpected that infection by S . Enteritidis may result in the surge of systemic IgE , which production is primarily implemented via the Th2 signaling pathway ( Shakib et al . , 2008 ) . On the contrary , it is generally accepted that bacteria such as S . Enteritidis induce mainly Th1 - cell - dependent cellular and hu"

  • Source
    • "In contrast, the observation that circulating levels of IgE often correlate with severe rather than uncomplicated malaria suggests a pathogenic role of IgE [15,16]. A recent study from malaria endemic areas of Gabon and India showed that circulating levels of total IgE do not appear to correlate with protection or pathology of malaria [17]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Background Both helminth and malaria infections result in a highly polarized immune response characterized by IgE production. This study aimed to investigate the total serum IgE profile in vivo as a measure of Th2 immune response in malaria patients with and without helminth co-infection. Methods A cross sectional observational study composed of microscopically confirmed malaria positive (N = 197) and malaria negative (N = 216) apparently healthy controls with and without helminth infection was conducted at Wondo Genet Health Center, Southern Ethiopia. A pre-designed structured format was utilized to collect socio-demographic and clinical data of the subjects. Detection and quantification of helminths, malaria parasites and determination of serum IgE levels were carried out following standard procedures. Results Irrespective of helminth infection, individuals infected by malaria showed significantly high levels of serum IgE compared with malaria free apparently healthy controls (with and without helminth infections). Moreover, malaria patients co-infected with intestinal helminths showed high level of serum IgE compared with those malaria patients without intestinal helminths (2198 IU/ml versus 1668 IU/ml). A strong statistically significant association was observed between malaria parasite density and elevated serum IgE levels (2047 IU/ml versus 1778 IU/ml; P = 0.001) with high and low parasitaemia (parasite density >50,000 parasite/μl of blood), respectively. Likewise, helminth egg loads were significantly associated with elevated serum IgE levels (P = 0.003). Conclusions The elevated serum IgE response in malaria patients irrespective of helminth infection and its correlation with malaria parasite density and helminth egg intensity support that malaria infection is also a strong driver of IgE production as compared to helminths.
    Parasites & Vectors 05/2014; 7(1):240. DOI:10.1186/1756-3305-7-240 · 3.43 Impact Factor
  • Source
    • "For instance, many people suffer from different allergic diseases, e.g., allergic rhinitis, which may cause extremely uncomfortable feelings and poor quality of life. Clinically, the concentration of immunoglobulin E (IgE) protein is one of the important indicators to reveal the allergic level in human serum [4–8]. Many conventional commercial allergy measurement techniques are available to analyze IgE concentration, e.g., enzyme-linked immunosorbent assay (ELISA) [9], surface plasmon resonance (SPR) [10], quartz crystal microbalance (QCM) [11] sensing techniques, chemiluminescence immunoassay (CLIA) [12], and Abbott AxSYM fluorescence polarization immunoassay (FPIA) [13], etc. "
    [Show abstract] [Hide abstract]
    ABSTRACT: A protein concentration measurement system with two-port flexural plate-wave (FPW) biosensors using a frequency-shift readout technique is presented in this paper. The proposed frequency-shift readout method employs a peak detecting scheme to measure the amount of resonant frequency shift. The proposed system is composed of a linear frequency generator, a pair of peak detectors, two registers, and a subtractor. The frequency sweep range of the linear frequency generator is limited to 2 MHz to 10 MHz according to the characteristics of the FPW biosensors. The proposed frequency-shift readout circuit is carried out on silicon using a standard 0.18 μm CMOS technology. The sensitivity of the peak detectors is measured to be 10 mV. The power consumption of the proposed protein concentration measurement system is 48 mW given a 0.1 MHz system clock.
    Sensors 12/2012; 13(1):86-105. DOI:10.3390/s130100086 · 2.25 Impact Factor
Show more