De Coppi, P. et al. Isolation of amniotic stem cell lines with potential for therapy. Nat. Biotech. 25, 100-106

Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157-1094, USA.
Nature Biotechnology (Impact Factor: 41.51). 02/2007; 25(1):100-6. DOI: 10.1038/nbt1274
Source: PubMed


Stem cells capable of differentiating to multiple lineages may be valuable for therapy. We report the isolation of human and rodent amniotic fluid-derived stem (AFS) cells that express embryonic and adult stem cell markers. Undifferentiated AFS cells expand extensively without feeders, double in 36 h and are not tumorigenic. Lines maintained for over 250 population doublings retained long telomeres and a normal karyotype. AFS cells are broadly multipotent. Clonal human lines verified by retroviral marking were induced to differentiate into cell types representing each embryonic germ layer, including cells of adipogenic, osteogenic, myogenic, endothelial, neuronal and hepatic lineages. Examples of differentiated cells derived from human AFS cells and displaying specialized functions include neuronal lineage cells secreting the neurotransmitter L-glutamate or expressing G-protein-gated inwardly rectifying potassium channels, hepatic lineage cells producing urea, and osteogenic lineage cells forming tissue-engineered bone.

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Available from: Mohummad Minhaj Siddiqui, Apr 17, 2014
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    • "Postnatal sources of stem cells can be reached from different kinds of tissues including bone marrow, brain, adipose tissue, umbilical cord blood and amniotic fluid [2] [3] [4] [5] [6]. The umbilical cord is an extraembryonic structure that supplies feeding for the fetus during the intrauterine life. "
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    ABSTRACT: Wharton's Jelly-derived Mesenchymal stem cells (WJ-MSCs) have been found to be an alternative source of stem cells for the regenerative medicine. This is as WJ-MSCs have potential for differentiation, self-renewal and unique immunomodulatory properties. Aim of the work: This work was conducted to assess our lab and staff capabilities in our Tissue Culture and Medical Genetics Unit to isolate, culture, identify and characterize WJ-MSCs derived from the adult human umbilical cord to be used for clinical applications in the future. Material and Methods: WJ-MSCs were isolated from the human umbilical cord collected from late-gestation pregnancy after cesarean section. Isolated WJ-MSCs cultured as P0 and recultured as P1. After confluency, WJ-MSCs were collected and characterized for surface markers expression using flow cytometry technique. Results: WJ-MSCs isolated in this study were positive for CD44, CD90, CD105 and negative for CD34. These findings suggest that WJ-MSCs appeared to be readily obtainable and highly proliferative cell lines that come from noninvasive source. Conclusion: isolated WJ-MSCs may be a good noninvasive source for stem cell therapy and regenerative medicine in the future. However, further studies should be done to evaluate its use as an allogenic cell source and expression of other markers in relation to cell immunogenicity.
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    • "the mouse subretinal space, could lead to teratomas formation (Arnhold et al. 2004). Among extra-embryonic tissues, amniotic fluid may represent a suitable source of mesenchymal stem cells (MSCs), characterized by carrying minimal somatic mutations and by showing high differentiative potential (Orciani et al. 2011; De Coppi et al. 2007). These cells are able not only to differentiate into cells of mesenchymal lineages in response to specific culture conditions in vitro but also into endoderm-derived cells, such as hepatocytes and astrocytes , olygodendrocytes and neurons (Zagoura et al. 2012; Rennie et al. 2013; Orciani et al. 2011; Salvolini et al., 2011). "
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    ABSTRACT: Dysfunction of the retinal pigmented epithelium (RPE) is one of the first effects of dry age-related macular degeneration (AMD) with consequent blindness. Hence, patients affected by this retinal disorder could benefit from a cell-based transplantation strategy for RPE. Actually, an effective protocol to approach this problem is lacking, though recently, it has been postulated the existence of a subpopulation of RPE stem cells (RPESCs) derived from adult RPE and able to reconstitute a functional RPE. On the other hand, the evidence related to the differentiative potential of human mesenchymal stem cells (MSCs) is continuously increasing. Among others, amniotic fluid-derived MSCs (AF-MSCs) may be a promising candidate, since these cells are characterized by high proliferation and differentiative potential. In this study, AF-MSCs and RPESCs were isolated, characterized to assay their stemness and induced to neuronal/retinal differentiation; specific RPE markers were then analyzed. Our results indicate that RPESCs are more suitable candidates for RPE replacement than AF-MSCs.
    Cell and Tissue Research 04/2015; 362(1). DOI:10.1007/s00441-015-2185-9 · 3.57 Impact Factor
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    • "AFSCs control the process of neurogenesis that lead to the improvement of the disorder (1). De Coppi et al. (2007) also worked on the osteogenic lineage of AFSCs. These cells were cultured in a medium that differentiated the cells in osteogenic lineage. "
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    ABSTRACT: Context: Amniotic Fluid Derived Stem Cells (AFSC) has mesenchymal origin and is multipotent. Having played their role in the detection of genetic abnormalities in the unborn children, they are gaining attention in the regenerative medicine because of their pluripotency. Evidence Acquisition: AFSCs possess great proliferating ability and have no ethical and religious issues in their use. AFSCs may also be studied for the stem cells differentiation such as production of multiple lineages of different cells like heart, liver, pancreas, etc. The potential of their use in regenerative medicine as well as their differentiation into multiple cells is possible. Results: AFSCs have the potential to be used in tissue repair and regeneration of bladder and kidney injuries, for the treatment of congenital anomalies like tracheal anomalies and spina bifida therapy etc. However, like every therapeutic potential, AFSCs also have some limitations such as low rate of differentiation of transplanted AFSCs and immune rejection. Conclusions: AFSCs have great therapeutic potential, but extensive research is warranted to overcome the limitations to use AFSC as therapy.
    02/2015; 3(1). DOI:10.5812/rijm.20135
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