De Coppi, P. et al. Isolation of amniotic stem cell lines with potential for therapy. Nat. Biotech. 25, 100-106

Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157-1094, USA.
Nature Biotechnology (Impact Factor: 41.51). 02/2007; 25(1):100-6. DOI: 10.1038/nbt1274
Source: PubMed

ABSTRACT Stem cells capable of differentiating to multiple lineages may be valuable for therapy. We report the isolation of human and rodent amniotic fluid-derived stem (AFS) cells that express embryonic and adult stem cell markers. Undifferentiated AFS cells expand extensively without feeders, double in 36 h and are not tumorigenic. Lines maintained for over 250 population doublings retained long telomeres and a normal karyotype. AFS cells are broadly multipotent. Clonal human lines verified by retroviral marking were induced to differentiate into cell types representing each embryonic germ layer, including cells of adipogenic, osteogenic, myogenic, endothelial, neuronal and hepatic lineages. Examples of differentiated cells derived from human AFS cells and displaying specialized functions include neuronal lineage cells secreting the neurotransmitter L-glutamate or expressing G-protein-gated inwardly rectifying potassium channels, hepatic lineage cells producing urea, and osteogenic lineage cells forming tissue-engineered bone.

Download full-text


Available from: Mohummad Minhaj Siddiqui, Apr 17, 2014
1 Follower
52 Reads
  • Source
    • "the mouse subretinal space, could lead to teratomas formation (Arnhold et al. 2004). Among extra-embryonic tissues, amniotic fluid may represent a suitable source of mesenchymal stem cells (MSCs), characterized by carrying minimal somatic mutations and by showing high differentiative potential (Orciani et al. 2011; De Coppi et al. 2007). These cells are able not only to differentiate into cells of mesenchymal lineages in response to specific culture conditions in vitro but also into endoderm-derived cells, such as hepatocytes and astrocytes , olygodendrocytes and neurons (Zagoura et al. 2012; Rennie et al. 2013; Orciani et al. 2011; Salvolini et al., 2011). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Dysfunction of the retinal pigmented epithelium (RPE) is one of the first effects of dry age-related macular degeneration (AMD) with consequent blindness. Hence, patients affected by this retinal disorder could benefit from a cell-based transplantation strategy for RPE. Actually, an effective protocol to approach this problem is lacking, though recently, it has been postulated the existence of a subpopulation of RPE stem cells (RPESCs) derived from adult RPE and able to reconstitute a functional RPE. On the other hand, the evidence related to the differentiative potential of human mesenchymal stem cells (MSCs) is continuously increasing. Among others, amniotic fluid-derived MSCs (AF-MSCs) may be a promising candidate, since these cells are characterized by high proliferation and differentiative potential. In this study, AF-MSCs and RPESCs were isolated, characterized to assay their stemness and induced to neuronal/retinal differentiation; specific RPE markers were then analyzed. Our results indicate that RPESCs are more suitable candidates for RPE replacement than AF-MSCs.
    Cell and Tissue Research 04/2015; 362(1). DOI:10.1007/s00441-015-2185-9 · 3.57 Impact Factor
  • Source
    • "AFSCs control the process of neurogenesis that lead to the improvement of the disorder (1). De Coppi et al. (2007) also worked on the osteogenic lineage of AFSCs. These cells were cultured in a medium that differentiated the cells in osteogenic lineage. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Context: Amniotic Fluid Derived Stem Cells (AFSC) has mesenchymal origin and is multipotent. Having played their role in the detection of genetic abnormalities in the unborn children, they are gaining attention in the regenerative medicine because of their pluripotency. Evidence Acquisition: AFSCs possess great proliferating ability and have no ethical and religious issues in their use. AFSCs may also be studied for the stem cells differentiation such as production of multiple lineages of different cells like heart, liver, pancreas, etc. The potential of their use in regenerative medicine as well as their differentiation into multiple cells is possible. Results: AFSCs have the potential to be used in tissue repair and regeneration of bladder and kidney injuries, for the treatment of congenital anomalies like tracheal anomalies and spina bifida therapy etc. However, like every therapeutic potential, AFSCs also have some limitations such as low rate of differentiation of transplanted AFSCs and immune rejection. Conclusions: AFSCs have great therapeutic potential, but extensive research is warranted to overcome the limitations to use AFSC as therapy.
    02/2015; 3(1). DOI:10.5812/rijm.20135
  • Source
    • "Despite the high proliferative rate of AFMSCs, they are still able to retain a normal karyotype with no evidence of tumorigenicity (Roubelakis et al., 2007). Human AFS cells express both markers of mesenchymal and pluripotent stem cells origin, such as stage-specific embryonic antigen (SSEA)-4 and Oct-4 (De Coppi et al., 2007). Once cultured in adherence however, they do not express markers of hematopoietic lineage such as CD45, CD34, and CD133 and express CD29, CD44, CD73, CD90, and CD105 (De Coppi, 2013). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Congenital diseases are responsible for over a third of all pediatric hospital admissions. Advances in prenatal screening and molecular diagnosis have allowed the detection of many life-threatening genetic diseases early in gestation. In utero transplantation (IUT) with stem cells could cure affected fetuses but so far in humans, successful IUT using allogeneic hematopoietic stem cells (HSCs), has been limited to fetuses with severe immunologic defects and more recently IUT with allogeneic mesenchymal stem cell transplantation, has improved phenotype in osteogenesis imperfecta. The options of preemptive treatment of congenital diseases in utero by stem cell or gene therapy changes the perspective of congenital diseases since it may avoid the need for postnatal treatment and reduce future costs. Amniotic fluid stem (AFS) cells have been isolated and characterized in human, mice, rodents, rabbit, and sheep and are a potential source of cells for therapeutic applications in disorders for treatment prenatally or postnatally. Gene transfer to the cells with long-term transgenic protein expression is feasible. Recently, pre-clinical autologous transplantation of transduced cells has been achieved in fetal sheep using minimally invasive ultrasound guided injection techniques. Clinically relevant levels of transgenic protein were expressed in the blood of transplanted lambs for at least 6 months. The cells have also demonstrated the potential of repair in a range of pre-clinical disease models such as neurological disorders, tracheal repair, bladder injury, and diaphragmatic hernia repair in neonates or adults. These results have been encouraging, and bring personalized tissue engineering for prenatal treatment of genetic disorders closer to the clinic.
    Frontiers in Pharmacology 12/2014; 5. DOI:10.3389/fphar.2014.00270 · 3.80 Impact Factor
Show more