Clinical significance of RET/PTC and p53 protein expression in sporadic papillary thyroid carcinoma

Division of Endocrinology, Hospital General i Universitari Vall d'Hebron, Barcelona, Spain.
Histopathology (Impact Factor: 3.3). 02/2007; 50(2):225-31. DOI: 10.1111/j.1365-2559.2006.02555.x
Source: PubMed

ABSTRACT Rearranged during Transfection (RET)/papillary thyroid carcinoma (PTC) and p53 are two genes involved in the pathogenesis of PTC. It has been suggested that RET/PTC expression is associated with higher rates of local extension and lymph node involvement, whereas p53 mutations are more frequent in poorly differentiated and anaplastic carcinomas. In addition, experimental studies have shown that p53 activity can modify the behaviour of PTC carrying RET/PTC. The aim of this study was to investigate the expression of both RET/PTC and p53 in order to evaluate their usefulness as prognostic factors.
Resected specimens of 61 cases of PTC were studied immunohistochemically using a polyclonal antibody to RET and a monoclonal antibody to p53 protein. RET/PTC expression was associated with extrathyroid extension of PTC, at diagnosis (P < 0.05). In contrast, no relationship between p53 immunoreactivity and clinical status was found. In addition, p53 expression was more prevalent among RET/PTC+ patients, and significantly influenced the relationship observed between RET/PTC and extrathyroid extension of the disease.
Our results suggest that immunohistochemistry for both PTC/RET and p53 could be useful in the clinical evaluation of patients with PTC.

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Available from: Jordi Mesa, Feb 13, 2015
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    • "e mean number of lymph node metastases was present ( Morita et al . , 2008 ) . Horie et al . ( 2001 ) also said that overexpression of p53 protein significantly correlated with large tumor size and the presence of capsular invasion ( Horie et al . , 2001 ) . But several studies ( Park et al . , 1998 ; Kalidag et al . , 2007 ; Jung et al . , 2007 ; Zafon et al . , 2007 ; Cvejic et al . , 2008 ; Hamzany et al . , 2012 ) found no association between p53 positivity and clinicopathologic data . In this study all parameters ( age , tumor size , multiplicity , extrathyroidal extension , vascular invasion , lymph node metastasis and mean number of metastatic lymph node ) revealed no significant correlation w"
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    ABSTRACT: Background: p53 protein expression has been detected immunohistochemically in papillary thyroid carcinoma(PTC). We investigated the relations between its expression and clinicopathologic features and its significance as a diagnostic marker. Materials and Methods: We compared and evaluated 93 patients in whom thyroidectomy with lymph node dissection had been performed to treat PTC for clinicopathologic significance and 102 patients with 23 papillary thyroid overt carcinomas (POC), 57 papillary thyroid microcarcinomas(PMC), 5 follicular adenomas (FA), 5 Hashimoto's thyroiditis (HT) and 12 nodular hyperplasias (NH) for significance as a diagnostic marker. Expression of p53 protein was evaluated immunohistochemically in sections of paraffin- embedded tissue. Results: Statistical analysis showed significantly different expression of p53 in PTC versus other benign thyroid lesions (BTL).The diagnostic sensitivity and specificity were 85.0% and 72.7%, respectively. Overexpression of p53 protein was observed in 44 of the 93 PTC cases (47.3%), but no significant correlation between p53 protein overexpression and clinicopathologic features (age, size, multiplicity, lymph node metastasis, extrathyroidal extension and vascular invasion) was noted. Conclusions: p53 is valuable to distinguish PTC from other BTL, but there is no correlation between p53 protein overexpression and clinicopathologic features.
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