Article

Efficient automatic noise reduction of electron tomographic reconstructions based on iterative median filtering

The Burnham Institute of Medical Research, La Jolla, CA 92037, USA.
Journal of Structural Biology (Impact Factor: 3.23). 06/2007; 158(2):196-204. DOI: 10.1016/j.jsb.2006.10.030
Source: PubMed

ABSTRACT A simple, fast and efficient noise-reduction protocol for three-dimensional electron tomographic reconstructions of biological material is presented. The approach is based on iterative application of median filtering and shows promise for automatic noise reduction as a pre-processor for automated data analysis tools which aim at segmentation, feature extraction and pattern recognition. The application of this algorithm produces encouraging results for a wide variety of experimental and synthetic electron tomographic reconstructions.

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