Visualization of corticofugal projections during early cortical development in a τ-GFP-transgenic mouse

UCLA Semel Institute for Neuroscience, 635 Charles E Young Drive South, Los Angeles, CA 90095-7332, USA.
European Journal of Neuroscience (Impact Factor: 3.67). 02/2007; 25(1):17-30. DOI: 10.1111/j.1460-9568.2006.05258.x
Source: PubMed

ABSTRACT The first postmitotic neurons in the developing neocortex establish the preplate layer. These early-born neurons have a significant influence on the circuitry of the developing cortex. However, the exact timing and trajectory of their projections, between cortical hemispheres and intra- and extra-cortical regions, remain unresolved. Here, we describe the creation of a transgenic mouse using a 1.3 kb golli promoter element of the myelin basic protein gene to target expression of a tau-green fluorescent protein (GFP) fusion protein in the cell bodies and processes of pioneer cortical neurons. During embryonic and early neonatal development, the timing and patterning of process extension from these neurons was examined. Analysis of tau-GFP fluorescent fibers revealed that progression of early labeled projections was interrupted unexpectedly by transient pauses at the corticostriatal and telencephalic-diencephalic boundaries before invading the thalamus just prior to birth. After birth the pioneering projections differentially invaded the thalamus, excluding some nuclei, e.g. medial and lateral geniculate, until postnatal days 10-14. Early labeled projections were also found to cross to the contralateral hemisphere as well as to the superior colliculus. These results indicate that early corticothalamic projections appear to pause before invading specific subcortical regions during development, that there is developmental regulation of innervation of individual thalamic nuclei, and that these early-generated neurons also establish early projections to commissural and subcortical targets.

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    • "d Rakic , 1990 ; Allendoerfer and Shatz , 1994 ; Kanold and Luhmann , 2010 ) . After the second waiting period at the RTN , CTA invade the thalamus , a process that takes several days with most thalamic nuclei being innervated postnatally in rats , mice , and hamsters ( Miller et al . , 1993 ; Molna´r et al . , 1998a ; Molna´r and Cordery , 1999 ; Jacobs et al . , 2007 ) . Candidate guidance cues that direct axons from specific cortical regions to the thalamic nuclei that they connect to in adulthood are beginning to be identified . Ephs and ephrins , semaphorins , slits and netrin pathways are differentially expressed between the LGN and MGN ( Horng et al . , 2009 ) . Therefore , distinct guidance cu"
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    Neuroscience 09/2013; 254. DOI:10.1016/j.neuroscience.2013.08.070 · 3.33 Impact Factor
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    • "Finally, neurons reach their correct position below the marginal zone (MZ) and stop migrating. Cortical neurons develop their projections during late embryogenesis (Lopez-Bendito and Molnar 2003; Jacobs et al. 2007; Grant et al. 2012), while cortical lamination is still in process. Corticofugal axons, from projection neurons in layers V and VI, are classified by their subcortical destinations into corticothalamic, corticotectal, corticospinal and corticopontine tracts (Molyneaux et al. 2007). "
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    Cerebral Cortex 08/2013; 25(1). DOI:10.1093/cercor/bht209 · 8.67 Impact Factor
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    • "Adapted from Jacobs et al. (2007) "
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