The effect of whey isolate on strength, body composition and plasma glutamine

Exercise Metabolism Unit, Center for Ageing, Rehabilitation, Exercise and Sport (CARES), Australia.
International journal of sport nutrition and exercise metabolism (Impact Factor: 2.44). 11/2006; 16(5):494-509. DOI: 10.1097/00005768-200205001-01688
Source: PubMed


Different dietary proteins affect whole body protein anabolism and accretion and therefore, have the potential to influence results obtained from resistance training. This study examined the effects of supplementation with two proteins, hydrolyzed whey isolate (WI) and casein (C), on strength, body composition, and plasma glutamine levels during a 10 wk, supervised resistance training program. In a double-blind protocol, 13 male, recreational bodybuilders supplemented their normal diet with either WI or C (1.5 gm/kg body wt/d) for the duration of the program. Strength was assessed by 1-RM in three exercises (barbell bench press, squat, and cable pull-down). Body composition was assessed by dual energy X-ray absorptiometry. Plasma glutamine levels were determined by the enzymatic method with spectrophotometric detection. All assessments occurred in the week before and the week following 10 wk of training. Plasma glutamine levels did not change in either supplement group following the intervention. The WI group achieved a significantly greater gain (P < 0.01) in lean mass than the C group (5.0 +/- 0.3 vs. 0.8 +/- 0.4 kg for WI and C, respectively) and a significant (P < 0.05) change in fat mass (-1.5 +/- 0.5 kg) compared to the C group (+0.2 +/- 0.3 kg). The WI group also achieved significantly greater (P < 0.05) improvements in strength compared to the C group in each assessment of strength. When the strength changes were expressed relative to body weight, the WI group still achieved significantly greater (P < 0.05) improvements in strength compared to the C group.

Download full-text


Available from: Andrew D Williams,
  • Source
    • "Highly digestible and quickly absorbed by the body, the WPs are widely used in situations of metabolic stress [4]. Whey-protein concentrates (WPCs) and whey-protein isolates (WPIs) usually contain a cysteine concentration at least four times higher than other high-quality proteins, and it produces an improvement in immune response [5]. Crohn's disease (CD) is a complex chronic inflammatory disease, with periods of exacerbation [6], which affects the gastrointestinal tract, and may also involve the musculoskeletal system, skin, and eyes [7]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: The objective of this study was to evaluate the acceptability of an alimentary supplement of bovine whey-protein concentrate (WPC) and TGF-β, unavailable commercially, by patients with Crohn’s disease (CD) and determine the chemical composition, solubility, and total amino acids content. The supplement was diluted in water, and an acceptance test was done to evaluate the aroma, flavour, and viscosity of the product using facial hedonic scale (nine-point scale), applied on 54 CD patients. The supplement composition indicated 73.3% protein, 10.5% fat, 2.2% ash, 6.3% water, and 7.7% carbohydrate. The supplement is presented as a good protein source and high content of essential amino acids. The average acceptance for all the attributes was between 5.0 and 6.0, and the flavour was mainly associated with soybean/grain, sour milk, and sweet/vanilla flavour. The results indicated that the supplement provided important nutritional properties for CD patients; however, for a large number of individuals to be encouraged to perform supplementation, it is essential to improve the sensory quality of the product. In order to do so, additional research is necessary to prevent the formation of volatiles which cause off-flavours or to mask undesirable aromas/flavours found in it.
    07/2013; 2013. DOI:10.5402/2013/947865
  • Source
    • "In a longer-term 6 month study of weight loss in overweight, older women, 2 × 25 g/day whey protein isolate in conjunction with an energy restricted diet and exercise training resulted in both a trend towards greater weight loss, and MRI-assessed adipose loss and gain in leg muscle mass [135]. Perhaps unexpectedly there is as yet limited evidence even in young healthy participants and bodybuilders that protein supplementation in combination with resistance exercise training does lead to greater gains in skeletal muscle mass [136-138], despite the growing literature supporting effects on protein synthesis, degradation and balance. In a study of elderly men undertaking a 12 week progressive resistance type exercise program, the addition of a protein supplement, perhaps surprisingly, did not further increase skeletal muscle hypertrophy and enhance lean body mass [139]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Epidemiological evidence shows that consumption of dairy products is associated with decreased prevalence of metabolic related disorders, whilst evidence from experimental studies points towards dairy protein as a dietary component which may aid prevention of type 2 diabetes (T2DM). Poor metabolic health is a common characteristic of overweight, obesity and aging, and is the forerunner of T2DM and cardiovascular disease (CVD), and an ever increasing global health issue. Progressive loss of metabolic control is evident from a blunting of carbohydrate, fat and protein metabolism, which is commonly manifested through decreased insulin sensitivity, inadequate glucose and lipid control, accompanied by a pro-inflammatory environment and hypertension. Adverse physiological changes such as excess visceral adipose tissue deposition and expansion, lipid overspill and infiltration into liver, muscle and other organs, and sarcopaenia or degenerative loss of skeletal muscle mass and function all underpin this adverse profile. 'Sarcobesity' and sarcopaenic diabetes are rapidly growing health issues. As well as through direct mechanisms, dairy protein may indirectly improve metabolic health by aiding loss of body weight and fat mass through enhanced satiety, whilst promoting skeletal muscle growth and function through anabolic effects of dairy protein-derived branch chain amino acids (BCAAs). BCAAs enhance muscle protein synthesis, lean body mass and skeletal muscle metabolic function. The composition and processing of dairy protein has an impact on digestion, absorption, BCAA kinetics and function, hence the optimisation of dairy protein composition through selection and combination of specific protein components in milk may provide a way to maximize benefits for metabolic health.
    Nutrition & Metabolism 07/2013; 10(1):46. DOI:10.1186/1743-7075-10-46 · 3.26 Impact Factor
  • Source
    • "VPX also contains whey protein isolate, which is higher in quality compared to whey protein concentrate. When combined with resistance training, whey protein isolate has been shown to result in significantly greater gains in lean mass and strength compared to casein [34]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Background Post-workout nutrient timing and macronutrient selection are essential for recovery, glycogen replenishment and muscle protein synthesis (MPS). Performance repeatability, particularly after strenuous activity, can be influenced by substrate availability, recovery markers and perceived rate of exertion. This study compared the differential effects of a complex protein ready-to-drink beverage (VPX) and isocaloric carbohydrate beverage (iCHO) on performance—agility T-test, push-up test, 40-yard sprint, and rate of perceived exertion (RPE), following high-intensity resistance training (HIRT). Methods In a randomized, double blind two-arm crossover controlled trial, 15 subjects performed a 15–18 minute (2:1 work to rest) HIRT and then immediately drank one of the two treatments. After a 2-hour fast, subjects returned to execute the field tests and report RPE. The protocol was repeated one week later with the other treatment. Results There were no significant main effect differences in the agility T-test (p = 0.83), push-up (p = 0.21) sprint (p = 0.12), average agility RPE (p = 0.83), average push-up RPE (p = 0.81) or average sprint RPE (p = 0.66) between the two trials and the two treatments. The multivariate analysis yielded a cumulative significant interaction effect amongst the three performance variables after consuming VPX (p < 0.01). These results suggest a complex protein beverage is a better post-workout choice compared to an isocaloric carbohydrate beverage for repeated performance for activities that require multiple energy demands and athletic skills; however, this outcome was not observed for each single performance event or RPE. Conclusion When considering the collective physical effects of the agility T-test, push-up and sprint tests, a complex protein beverage may provide a recovery advantage as it relates to repeated-bout performance compared to an iCHO-only beverage. Additional research examining the chronic effects of post-exercise protein versus iCHO beverages on performance repeatability, particularly in special populations (e.g. tactical and elite athletes), is warranted to further develop these findings.
    Journal of the International Society of Sports Nutrition 06/2013; 10(1):31. DOI:10.1186/1550-2783-10-31 · 1.91 Impact Factor
Show more