Carbamylated Low-Density Lipoprotein Induces Monocyte Adhesion to Endothelial Cells Through Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1

University of Arkansas at Little Rock, Little Rock, Arkansas, United States
Arteriosclerosis Thrombosis and Vascular Biology (Impact Factor: 6). 05/2007; 27(4):826-32. DOI: 10.1161/01.ATV.0000258795.75121.8a
Source: PubMed

ABSTRACT Carbamylated low-density lipoprotein (LDL), the most abundant modified LDL isoform in human blood, has been recently implicated in causing the atherosclerosis-prone injuries to endothelial cells in vitro and atherosclerosis in humans. This study was aimed at testing the hypothesis that carbamylated LDL acts via inducing monocyte adhesion to endothelial cells and determining the adhesion molecules responsible for the recruitment of monocytes.
Exposure of human coronary artery endothelial cells with carbamylated LDL but not native LDL caused U937 monocyte adhesion and the induction of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 adhesion molecules as measured by cell enzyme-linked immunosorbent assay. Silencing of intercellular adhesion molecule-1 by siRNA or its inhibition using neutralizing antibody resulted in decreased monocyte adhesion to the endothelial cells. Similar silencing or neutralizing of vascular cell adhesion molecule-1 alone did not have an effect but was shown to contribute to intercellular adhesion molecule-1 when tested simultaneously.
Taken together, these data provide evidence that intercellular adhesion molecule-1 in cooperation with vascular cell adhesion molecule-1 are essential for monocyte adhesion by carbamylated low-density lipoprotein-activated human vascular endothelial cells in vitro.

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    • "For example vascular (VCAM), intercellular (ICAM), neural (NCAM) and mucosal addressin (MADCAM) cell adhesion molecules, as well as junction adhesion molecules (JAM) [14]. Some of these genes are also involved in immune cell adhesion, for example ICAM1 and VCAM1 are involved in monocyte-endothelial adhesion [15]. Moreover JAM genes are known to be involved in lymphocyte homing [16]. "
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    • "We found similar associations between temperature and sVCAM-1 to those between temperature and sICAM-1. Similarity of these findings was expected due to moderate correlation (0.40) between sICAM-1 and sVCAM-1, even though higher short-term reactivity of ICAM-1 than VCAM-1 after exposure to exogenous factors has been reported in cell studies [36]. "
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