Topical iodine facilitates transdermal delivery of insulin.
ABSTRACT Transdermal delivery of insulin is a non-invasive alternative to the subcutaneous injection of insulin in diabetic patients. It has been found that skin pretreatment with iodine followed by a dermal application of insulin results in reduced glucose and elevated hormone levels in the plasma. Topical iodine protects the dermally applied insulin presumably by inactivation of endogenous sulfhydryls such as glutathione and gamma glutamylcysteine which can reduce the disulfide bonds of the hormone. Thus, the effect of iodine is mediated by retaining the potency of the hormone during its penetration via the skin into the circulation. The proposed procedure might be applicable for additional disulfide-containing peptides such as calcitonin, somatostatin, oxytocin/vasopressin and their analogs.
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ABSTRACT: Abstract The aim of this study was to prepare ,biologically active insulin-loaded alginate microparticles by spray ,drying. Particles were ,produced ,from ,three alginate feed concentrations of 1, 1.5 and 2% w/v, with respective insulin loadings of 11.8, 7.8 and 5.8 mg/g of alginate and investigated in terms of mass yield, moisture content, particle size, morphology,and encapsulation efficiency. The mass yield of the system was determined tobe between 15 and 30%, with approximately 3% of the initial dry mass ending up in
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ABSTRACT: The present study deals with the development of transferosomal gel containing insulin by reverse phase evaporation method for painless insulin delivery for use in the treatment of insulin dependent diabetes mellitus. The effect of independent process variables like ratio of lipids (soya lecithin:cholesterol), ratio of lipids and surfactants, and ratio of surfactants (Tween 80:sodium deoxycholate) on the in vitro permeation flux (μg/cm(2)/h) of formulated transferosomal gels containing insulin through porcine ear skin was optimized using 2(3) factorial design. The optimal permeation flux was achieved as 13.50 ± 0.22 μg/cm(2)/h with drug entrapment efficiency of 56.55 ± 0.37% and average vesicle diameter range, 625-815 nm. The in vitro insulin permeation through porcine ear skin from these transferosomal gel followed zero-order kinetics (R (2) = 0.9232-0.9989) over a period of 24 h with case-II transport mechanism. The in vitro skin permeation of insulin from optimized transferosomal gel by iontophoretic influence (with 0.5 mA/cm(2) current supply) also provided further enhancement of permeation flux to 17.60 ± 0.03 μg/cm(2)/h. The in vivo study of optimized transferosomal gel in alloxan-induced diabetic rat has demonstrated prolonged hypoglycemic effect in diabetic rats over 24 h after transdermal administration.Saudi Pharmaceutical Journal 10/2012; 20(4):355-63. DOI:10.1016/j.jsps.2012.02.001 · 1.00 Impact Factor
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ABSTRACT: With view to develop an amphoteric charged membrane for drug delivery, we have studied the adsorption of insulin on it. In the present study, the amphoteric charged membranes were prepared by pore-surface modification of porous poly(acrylonitrile) (PAN) membranes by grafting with acrylic acid (AAc) and/or N,N-(dimethylamino)propyl acrylamide (DMAPAA). Their surface charge properties and the insulin adsorption behaviors were investigated by zeta potential measurement and UV spectrophotoscopy, respectively, at different pHs. The equilibrium adsorbed amount of insulin correlates well with the charge properties of the membranes and insulin, which indicates that electrostatic interaction played an important role in the insulin adsorption. In addition, adsorption kinetics changed from a Fickian mode to a non-Fickian one when adsorbed amount increased to very high values.Polymer Journal 07/2008; 40(9):837-841. DOI:10.1295/polymj.PJ2008093 · 1.55 Impact Factor